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Populism as a political style: analysis of Facebook communication of selected presidential candidates in the run-up to the 2023 Czech presidential elections
Drahota, Jan ; Křeček, Jan (advisor) ; Nečas, Vlastimil (referee)
This thesis analyses populist content as a political style. Populist content is examined in the Facebook communication of the candidates for the presidency of the Czech Republic in the 2023 elections. The period under study is November 2022, i.e. the pre-election period. Populism as a political style is represented by authors Benjamin Moffitt and the pair Jan Jagers and Stefaan Walgrave, who conceptualize characteristic elements of populism that can be analytically examined. The aim of this paper is to determine whether candidates use these elements in their Facebook communications, and if so, in what ways and to what extent. A secondary aim of the thesis is to determine the relationship of user interaction with each populist element. The theoretical part introduces the concept of populism and its possible understanding in academia. Furthermore, the theoretical part introduces social networks and the functioning of their infrastructure in terms of content dissemination. The practical part focuses on the communication of individual candidates and captures the phenomena under study, interpreting them in detail.
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Antigens in the sandfly saliva and antibody response of the bitten hosts
Drahota, Jan ; Volf, Petr (advisor) ; Kopecký, Jan (referee) ; Soares Maia, Carla Alexandra (referee)
Leishmaniases are neglected diseases occurring mainly in developing countries of Africa, Asia and Latin America. However, these diseases are also present in Europe and North America and due to climate changes and human activities they spread to higher latitudes and altitudes. In these theses, we review the current information about the spread of leishmaniases, its vectors and reservoirs in Europe. The risk of Leishmania transmission is closely connected with the host-vector contact. Recently, immunoglobulin G (IgG) antibody response to sandfly saliva has been proven as a reliable marker of the host exposure. However, sandfly saliva is a complex mixture of components with different chemical and antigenic properties and it is laborious and expensive to acquire. Therefore, we have focused on preparation of major salivary antigens in the form of recombinant proteins that would be capable to replace the saliva in immunological screenings. We choose two European vectors, Phlebotomus (P.) papatasi and Phlebotomus (P.) perniciosus and identified their major salivary antigens by western blotting and mass spectrometry. We expressed these proteins in the bacterial system and test their antigenicity using ELISA and western blotting with sera of mice and dogs bitten by these sand fly species. The most promising...
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Development trends of urban green vegetation in Prague
Drahota, Jan ; Franke, Daniel (advisor) ; Markéta, Markéta (referee)
Aim of this text is to create comprehensive knowledge about green vegetation on city scale and indicate developmental trends in this sphere in area of Prague. Another important parts of document are general-purpose methodology and analysis of city planning instruments. Data required for research are acquired by satellite land survey. Results of this work gives information about progress of city vegetation in urban space of Prague. Theses included comparison with other European metropolises.
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