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Shell shape variability in selected members of family Geoemydidae
Protiva, Tomáš ; Rehák, Ivan (advisor) ; Šmíd, Jiří (referee) ; Široký, Pavel. (referee)
Turtles always fascinated men primarily by morphology, unique embryogenesis and later also by uncertain phylogenetic position. As this group exists for 200 Mya, with taxon Odontochelys and Proganochelys identified as the first ancestors, recently they are endangered by habitat destruction and hunting for meat. The worst situation is in Asia, with China as the most important consumer of turtle meat and other products. Captive breeding programs may be the only chance for lot of almost extinct species. Proper taxonomic and geographical determination is crucial for correct management of rescue programs. Shell shape variability studied by geometric morphometric method and supported by genetics is the right tool for this task. My thesis is focused on verification of the use of geometric morphometric method for selected species of family Geoemydidae. This family has centre of distribution in Asia and many of its species are endangered by so called Asian Turtle Crisis. We found unusual high occurrence of supernumerary vertebral scute in Morenia petersi. Shape of the carapace and plastron is not affected by presence of this extra scute. There are no significant differences between turtles with additional scute and turtles with normal five vertebral layouts. Scute pattern with supernumerary vertebral scute...
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Systematics, Phylogeny and Biogeography of the gecko genus Hemidactylus (Reptilia, Gekkonidae) in Arabia and the Near East.
Šmíd, Jiří ; Moravec, Jiří (advisor) ; Kotlík, Petr (referee) ; Rehák, Ivan (referee)
(in English) Until relatively recently, the gecko genus Hemidactylus was considered to contain only several hard-to-distinguish species in the eastern Mediterranean and the Arabian Peninsula. This was mostly given by the apparent morphological uniformity of the geckos in combination with their often synanthropic lifestyle, which facilitated the dispersal of some of the species over large ranges. However, our understanding of the diversity of the genus was about to change with the onset of molecular phylogenetic methods that became extensively available and routinely applied to tackle the questions of systematics and phylogeny of all kinds of organisms. This thesis focuses on resolving the phylogeny, systematics, and biogeography of the Arabian Hemidactylus. Using an unprecedented sampling of over 500 samples of more than 40 Hemidactylus species from the eastern Mediterranean, Arabian Peninsula, Socotra, North and East Africa and Iran and sequencing up to six genes (two mitochondrial - 12S rRNA, cyt b; four nuclear - cmos, mc1r, rag1, rag2) I reconstructed the time-calibrated phylogeny of the arid clade of Hemidactylus, inferred its complex biogeographical history in the region, and detected seven potentially new species. The results of the biogeographic analyses indicate that the current...
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Identification DNA of Plant and Animal Species in Food by Polymerase Chain Reaction
Šmíd, Jiří ; Hanák,, Petr (referee) ; Timko,, Jozef (referee) ; Kuchta, Tomáš (advisor)
We were developing detection methods for three food allergens of plant origin. We used real-time PCR for soy detection in food oriented on gene lec, that is coding lektine specific for soy. On this target sequence were oriented PCR system with primers Le2F and Le2R and TaqMan probe Le2P. Detection limit (2,75 pg), practical detection limit (0,02 %), inclusivity and exclusivity were determined. Whole system were quantified. Real-time PCR for pistachio detection were based on primers and probe for gene COR. Detection limit (3,5 pg), practical detection limit (0,002 %), inclusivity and exclusivity were determined. For almond detection we were not succeed system, that fulfil all qualitative parametres.
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Possibilities of using modern methods in manufacturing of prototype castings
Šmíd, Jiří ; Horenský, Jiří (referee) ; Horáček, Milan (advisor)
The introduction part of the thesis focuses on the overview of rapid prototyping in foundry industry. Principles of the most important RP methods are described and the FDM method is analyzed in more detail. This method was used in the practical part for the production of wax patterns with silicone moulds. The wax patterns were used for the production of castings using the lost wax method. The result of this work is determination of dimensional changes during the whole process of casting manufacture from the drawing to the final casting.
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Development of transient transfection protocol for HEK293 EBNA1 cells
Šmíd, Jiří ; Španová, Alena (referee) ; Ševčík, Mojmír (advisor)
Recombinant proteins belong to considerable biofarmaceutics products used in biomedical research and in the treatment of human disease. Recombinant protines can be produced by stable transfection in big amount or by faster transient transfection with smaller amounts. To provide regular biological activity, it is necessary for the protein to be properly folded and post-translationally modified. As these modifications can be accurately performed only in mammalian cells, they have become the major host for complex r-protein expression. In this thesis is described transient transfection HEK 293 EBNA1 cells with linear polyethylenimines. These cells has been adapted to suspension cultivation in serum free medium. The cells were transfected with pcDNA3.1, pCI, pEBSV1, pCEP4, pEAK8 a pcDNA5/FRT/TO plasmids, everyone contained repoter gene SEAP. Concentration of SEAP in cell culture supernatants were determined in order to compare efficiencies of individual transfections. DNA:PEI ratio was another factor which was optimised and two different PEIs were compared. Highest achieved expresion was 50 mg per litre with transfection in 24 well plate when DNA:PEI ratio was 1:5. Comparison of six different plasmids give the bigest expresion pCEP4/SEAP, in well plate as well as in scaled up system.
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Metodika pro práci s přírodovědeckým typovým materiálem
Holec, Jan ; Kment, Petr ; Wagner, Jan ; Šmíd, Jiří ; Šída, Otakar ; Kvaček, Jiří ; Sejkora, Jiří ; Kuželka, Vítězslav
The book deals with basic, really existing elements of nomenclature in natural sciences - types, which represent pillars for scientific names of organisms and minerals. It describes especially the managing of types governed by zoological and botanical codes (ICZN, ICN), both for their living and extinct representatives. The text covers the following aspects of managing types: their distinguishing, terminology, preparation, preventive conservation, packaging, labelling, documentation, publishing, studying, and loaning.
Fulltext:  PDF
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Financial analysis of the enterprise Mlýn Havlíčkův Brod, s.r.o
Týcová, Hana ; Cimický, Jan (advisor) ; Šmíd, Jiří (referee)
The goal of this bachelor thesis is to carry out a financial analysis of the enterprise Mlýn Havlíčkův Brod, s.r.o. in years from 2003 to 2007. The thesis includes two parts: methodical and practical. The methodical part introduces the issue of financial analysis, what is financial analysis, what are the outcomes and what sources are needed. The practical part deals with the analysis itself and the results of it. First of all the company Mlýn Havlíčkův Brod is introduced and then the gained knowledge is applied and the analysis carried out. The outcome of the analysis is further evaluated and interpreted. The conclusion consists of analysis summary, its interpretation and suggested recommendations.
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