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Thermochemistry of high nitrogen heterocyclic compounds
Bartošková, Monika ; Pekař, Miloslav (referee) ; Friedl, Zdeněk (advisor)
The prediction of detonation properties of the new generation of high-nitrogen energetic materials (HNEM) is based on knowledge of their heats of formation, which are sum of values of particular nitrogen heterocyclic fragments. The diploma thesis describes theoretical calculations of heats of formation in gas phase ?f H°(298,g) for series of azines (number of N atoms 2-6) and azoles (number of N atoms 2-5) by means of quantum chemical methods. The semiempirical methods as PM3, DFT methods utilizing isodesmic approach and finally thermochemical G-recipes were used. All calculated values of heats of formation were scrutinized and for future application to HNEM materials the DFT B3LYP/cc-pVTZ method and thermochemical recipe T1 were recommended.
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Utilzation of physico-chemical analyses in study on microbial stress-response
Slaninová, Eva ; Pekař, Miloslav (referee) ; Sedláček, Petr (advisor)
This diploma thesis deals with the utilization of physico-chemical analysis in study on microbial stress–response. The main content of this work was to propose and optimize techniques and methods which are generally used in different industries. Two bacterial strains, Cupriavidus necator H16 and its mutant strain Cupriavidus necator PHB-4 producing polyhydroxyalkanoates (PHA) under certain conditions, were used as model microorganisms. Initially, microscopic teques such as transmission electron microscopy (TEM), cryo scanning electron microscopy (cryo SEM) and atomic force microscopy (AFM) were proposed and tested for the characteriozation of morphological differences of bacteria. Furthermore, viscoelastic properties of bacteria were determined and compared by the oscillatory tests of the rheology method considering another type of samples. Thermal analysis methods, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) in particular, focused on water transport and behavior of intracellular water influenced by presence of the granules of PHB in bacteria. The last technique, utilized in the study, was dynamic and electrophoretic light dispersion during pH changes for characterization of the surface properties of bacteria such as size, zeta potential and the isoelectric point.
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Preparation and characterization of complex liposomal for drug delivery systems
Szabová, Jana ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
This diploma thesis deals with the preparation and characterization of stealth liposomes and their combination with trimethylchitosan (TMC). This complex could find application in the field of inhalation administration. Stealth liposomes were prepared from neutral phophatidylcholine, negatively charged fosfatidic acid and polyethyleneglycol bounded to phosphatidylethanolamine. We have managed to prepare stealth liposomes with suitable properties that should guarantee passive targeting without evocation an immune response, despite the content of the negative component. We also found a suitable method of preparation for stealth liposome–TMC complex, where the change of size and zeta potential confirmed the non–covalent bound between two components despite the content of the polyethyleneglycol.
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Modelling of particle movement in liquid and hydrogel media
Orság, Miroslav ; Sedláček, Petr (referee) ; Pekař, Miloslav (advisor)
Main goal of this thesis was to became familiar with general mathematical description of particle motion in fluid and hydrogel media in COMSOL software. Simulate a basic Brownian motion of particles with various diameter in media with different density. According to software options, simulate motion in polymer structure or in viscoelastic media. Consider an options of the model for use in microrheology and suggest next process in this field.
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Soil salination and natural lignite as a potential remediating agent
Kořínková, Alena ; Sýkorová, Ivana (referee) ; Pekař, Miloslav (advisor)
This bachelor thesis deals with sorption ability of raw and water washed lignite for salt NaCl from water solution. Measurement was carried out in accordance with norm ČSN 11265 Quality of soil – Determination of electric conductivity with some modification (time period, extraction agent). Behaviour of lignite-water-NaCl, soil-water-NaCl and soil-lignite-water-NaCl mixtures was studied by measuring pH and electric conductivity.
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Study of Lignite Organic Matter
Doskočil, Leoš ; Maršálek, Roman (referee) ; Havelcová, Martina (referee) ; Pekař, Miloslav (advisor)
This work deals with the study of organic matter in the South Moravian lignite using the available physico-chemical methods and the results obtained are eventually considered for non-energy applications of lignite. Lignite organic matter was studied by degradation methods, which included CuO oxidation, thermochemolysis with TMAH and DFRC method. Special attention was paid to the lignite oxidation with hydrogen peroxide, which was carried out under different reaction conditions (time and temperature). Thermal degradation was performed in an inert atmosphere in order to observation of chemical change of lignite (including lignite after extraction with chloroform, demineralised lignite, remineralised lignete) during pyrolysis by means of various methods, especially TG-FTIR for analysis of gaseous products and FTIR for analysis the residual lignite after pyrolysis. Further, water-soluble fractions from lignite were characterized, particularly in terms of the content of organic substances.
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Aggregation processes in amphiphil-polymer system
Šejnohová, Michaela ; Márová, Ivana (referee) ; Pekař, Miloslav (advisor)
This Bachelor´s thesis is focused on the exploration of polyelectrolyte-surfactant system by fluorescence spectroscopy with pyrene as fluorescent probe. Cationic cetyltrimethylammonium bromide (CTAB) was used as a surfactant. Native hyaluronan was used as polyelectrolyte. Hyaluronan was used in one constant molecular weight and one constant concentration. The whole system was prepared in an aqueous environment. Interactions were studied in area between critical aggregation concentration (CAC) and critical micelle concentration (CMC). The influences of the concentration of pyrene and the effect of the way of the samples preparation on aggregation behavior were also studied. It was found that above the critical aggregation concentration exists an area where stable properties of aggregates can be found. The concentration of pyrene has a major effect on the fluorescence properties of the system. Preparation of samples affects the aggregation of the system. At higher surfactant concentrations, above the stable area, was observed phase separation and gel formation.
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Preparation, characterization and evaluation of application potential of semiIPN hydrogels
Papežíková, Hana ; Pekař, Miloslav (referee) ; Sedláček, Petr (advisor)
Aim of this thesis os to optimize set up of hydrogel based on semi-interpenetrated polymer networks. Polyhydroxyethylmethyl acrylate was chosen as an model example of hydrogel due to its common use in biomedicine. At first, thermoiniciation was used for preparation and changed to photoiniciation afterwards. Following the optimisation of the hydrogel set up with proper qualities, the polyelectrolyte sodium polystyrene sulfonate was implanted. Object of research was its impact on structure, flowing and transport features of hydrogels. All qualities were tested by drying, rheology, and fluorescence correlation spectroscopy. Results clearly validate influence of hydrogel features by polyelectrolytes.
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Development of Material Based on Hyaluronic Acid s Hydrogels for Myocardial Regeneration
Kovářová, Lenka ; Kubala,, Lukáš (referee) ; Prokš,, Vladimír (referee) ; Pekař, Miloslav (advisor)
The thesis is focused on material development based on hyaluronic acid usable in regenerative medicine, especially for heart tissue regeneration after myocardial infarction. The object of the study is the oxidized form of hyaluronic acid (HA-Ox) and hydroxyphenyl derivative of HA (HA-TA). HA-Ox can be crosslinked with a bifunctional alkoxyamine POA and HA-TA undergoes an enzymatic reaction in the presence of hydrogen peroxide catalysed by horseradish peroxidase leading to gel formation. To describe the materials, chemical and physical properties, gelation kinetics and conditions of crosslinking reactions were studied. Hydrogels were characterized by mechanical and viscoelastic properties, degradability or stability in simulated body fluids. These hydrogels serve as scaffolds for the selected cell type. To promote cell adhesion and viability, an RGD sequence has been bonded to the structure of HA-TA. This resulting material is also compatible with selected applicators. Its viscosity and extrusion force are low enough to allow application with a catheter with a very small internal diameter. The applicability of the material through the supply tube to the hydrogel reservoir of the second SPREADS device showed good homogeneity, cell distribution and viability. Finally, the material was applied in vivo using these devices during a preclinical study.
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Carbon sorbents for biologically active substances
Lanžhotská, Aneta ; Sedláček, Petr (referee) ; Pekař, Miloslav (advisor)
This Bachelor thesis deals with the general characteristics of lignite and its definitions as a material system. Lignite represents geologically the youngest type of coal, and belongs to kaustobiolites, which are masses with varying degrees of carbonification of the original phytomass. The degree of carbonification is roughly between peat and brown coal. Particular attention was paid to the application of lignite as a cheaper and more versatile sorbent to replace activated carbon and for the use of sorption of biologically active substances in biotechnology. The specific structure and composition give the lignite the natural sorption capability. In model experiments, polyphenols were used as significant microbial inhibitors for which lignite had a high sorption and for glucose, which lignite practically did not absorb. A model experiment was carried out with a hydrolyzate of a corn biomass, where the lignite showed optimal sorption.
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