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Antimicrobial susceptibility testing of newly synthesized compounds
Benková, Markéta ; Marek, Jan (advisor) ; Žemličková, Helena (referee) ; Hanovcová, Irena (referee)
In the dissertation thesis we dealt with the antimicrobial susceptibility testing (against gram-positive and gram-negative bacteria, yeasts and filamentous fungi) of newly synthesized compounds and their structure-antimicrobial activity relationship monitoring. These compounds based on quaternary ammonium salts with various structural modifications were prepared in cooperation with the Department of Toxicology and Military Pharmacy of the Faculty of Military Health Sciences of the University of Defence and Biomedical Research Centre at the University Hospital Hradec Králové on a joint project of the Czech Health Research Council. Selection of a methodology for antimicrobial susceptibility testing was the first part of the thesis. The microdilution broth method, which was successfully implemented at the department, was chosen for this purpose. The in vitro testing of selected synthesized compounds against several bacterial and fungal strains was the next phase of the work. Several substances have also been evaluated for the green microalgae growth inhibition (in terms of environmental friendliness). In addition, cytotoxic effects were tested on mammalian cell line to assess whether the compounds were more suitable as antiseptics or surface disinfectants. Several substances active against individual...
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Modern women's narrative writing in Islamic countries (Iran - Saudi Arabia)
Vojtíšková, Věra ; Marek, Jan (advisor) ; Veselý, Rudolf (referee)
The aim of the thesis Modern Women's Narrative Writing in Islamic Countries (Iran - Saudi Arabia) is to compare the situation of women writers on the Iranian and Saudi literary stages, to give an overview of the literary activities of the women fiction writers from both countries, to track down thematic motifs in the chosen writers' works, and to compare the authors' approaches to particular motifs. In order that the view of the situation in both societies is complete, the thesis includes also a brief historical/political development of both states in the time relevant for the modern literary output, that is the 19th and 20th centuries till today. The second chapter serves the same purpose as the first one, it describes and compares the women's situation in the Iranian and Saudi societies, and the local women's movements, for there has always been a link between women's literary activities and their emancipatory efforts.
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Synthesis and Evaluation of Quaternary Nitrogen Compounds
Marek, Jan ; Cabal, Jiří (advisor) ; Doležal, Martin (referee) ; Navrátil, Leoš (referee)
Jan Marek Příprava a testování látek obsahujících kvarterní dusík Klíčová slova: Kationické tenzidy, syntéza, analýza, kvartérní dusík, micelární katalýza, desinfekce, dekontaminace Souhrn: Disertační práce se zabývá přípravou a testováním látek typu kationických tenzidů jako případných desinfekčních či dekontaminačních činidel. Jelikož kvartérní kationické tenzidy jsou látky široce užívané v mnoha odvětvích (farmacie, chemický průmysl, potravinářský průmysl atd.) jsou stále předmětem velkého zájmu. Bylo navrhnuto a připraveno více než 40 tenzidů na bázi kvartérního dusíku. Látky byly odvozeny od struktur dnes běžně používaných (benzalkonium, cetylpyridinium či cetyltrimetylammonium). Syntetická část plynule navazuje na mou diplomovou práci, kde jsem se zabýval přípravou podobných látek. Disertační práce však popisuje syntézu látek, které mohou mít do molekuly zavedenu i různou nukleofilní skupinu (hydroxylovou či oximovou). Bylo připraveno šest řad látek na bázi 6-hydroxychinolinu, 3-hydroxypyridinu, fenylethyldimethylaminu, 3-hydroxiiminomethylpyridinu, 4-hydroxiiminomethylpyridinu a 4-(-1-aminohydroxiiminomethyl)pyridinu. Každá řada obsahovala 7 homologů lišících se vždy o dvě metylénové jednotky (C8-C20). Připravené struktury byly potvrzeny analýzami NMR, EA či MS. Rovněž byla vyvinuta HPLC metodika pro...
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Antimicrobial susceptibility testing of newly synthesized compounds
Benková, Markéta ; Marek, Jan (advisor) ; Žemličková, Helena (referee) ; Hanovcová, Irena (referee)
In the dissertation thesis we dealt with the antimicrobial susceptibility testing (against gram-positive and gram-negative bacteria, yeasts and filamentous fungi) of newly synthesized compounds and their structure-antimicrobial activity relationship monitoring. These compounds based on quaternary ammonium salts with various structural modifications were prepared in cooperation with the Department of Toxicology and Military Pharmacy of the Faculty of Military Health Sciences of the University of Defence and Biomedical Research Centre at the University Hospital Hradec Králové on a joint project of the Czech Health Research Council. Selection of a methodology for antimicrobial susceptibility testing was the first part of the thesis. The microdilution broth method, which was successfully implemented at the department, was chosen for this purpose. The in vitro testing of selected synthesized compounds against several bacterial and fungal strains was the next phase of the work. Several substances have also been evaluated for the green microalgae growth inhibition (in terms of environmental friendliness). In addition, cytotoxic effects were tested on mammalian cell line to assess whether the compounds were more suitable as antiseptics or surface disinfectants. Several substances active against individual...
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Present authorship prehension of journalism genre of film review
Marek, Jan ; Kruml, Milan (advisor) ; Halada, Andrej (referee)
This thesis focuses on present prehension of publicistic genre of film review by its authors and seeks for its differences from original theoretical definitions including the reasoning and question on its future change. Derivation of our research is confirmation of hypothesis which was conclusion of previous bachelor thesis and it speaks of transformational tendency of film reviews in time. Combination of quantitative and qualitative technique gathering data, therefore questionnaire survey with present-day film reviewers and semi-structured interviews with well-known figures, aims to the most perfect capturing of their present output, equipment for it and values, but also theirs own authorship opinions about points of importance within writing about movies and how it is changing in time and eventually where it is heading.
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Reconstruction of Missing Parts of the Face Using Neural Network
Marek, Jan ; Drahanský, Martin (referee) ; Goldmann, Tomáš (advisor)
Cílem této práce je vytvořit neuronovou síť která bude schopna rekonstruovat obličeje z fotografií na kterých je část obličeje překrytá maskou. Jsou prezentovány koncepty využívané při vývoji konvolučních neuronových sítí a generativních kompetitivních sítí. Dále jsou popsány koncepty používané v neuronových sítích specificky pro rekonstrukci fotografií obličejů. Je představen model generativní kompetitivní sítě využívající kombinaci hrazených konvolučních vrstev a víceškálových bloků schopný realisticky doplnit oblasti obličeje zakryté maskou.
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A comparison of lethal and maximum tolerated dose of standard acetylcholinesterase reactivators
Žárská, Nikola ; Kovařík, Miroslav (advisor) ; Marek, Jan (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate Bc. Nikola Žárská Supervisor PharmDr. Miroslav Kovařík, Ph.D. Consultant Mgr. Martina Hrabinová Title of Thesis A comparison of lethal and maximum tolerated dose of standard acetylcholinesterase reactivators. Diploma thesis deals with the topic of nerve agents. Specifically, it demonstrates the reactivating effect of oxime HI-6 (at 100 % maximum tolerated dose and 5 % median lethal dose) on the acetylcholinesterase inhibited by sarin. The experiment was carried out in vivo in Balb/c mice. The treatment was administered either with oxime HI-6 alone, in the above- mentioned doses, or in combination with the parasympatholytic atropine. The change in activity of acetylcholinesterase was measured by spectrophotometrically method, modified by the Ellman, in blood and brain. There were no signs of sarin intoxication in mice, that where treated with 100 % maximum tolerated dose of HI-6, in compare to mice treated with atropine only and 5 % median lethal dose of HI-6, where the intoxication was observed. It was founded that after i.m administration of 100 % maximum tolerated dose, the HI-6 concentration reached 500 µM in plasma and 10 µM in brain. This concentration is safe, but for...
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Translation and analysis of a chapter from the novel The Red Knight by Miles Cameron
Marek, Jan ; Richterová, Jana (advisor) ; Ženíšek, Jakub (referee)
This thesis deals with the translation of a chapter from a book called The Red Knight (2012) from English to Czech. It consists of three parts - the introduction, the theoretical part, the practical part together with the final analysis of phenomena in my translation. In the first part, besides the introduction, there is a summary of the novel as a first part of the fantasy cycle The Traitor Son. The practical part contains a mirror translation of the selected chapter. The theoretical part of this thesis explores some aspects of translation and explains why the translation in the practical part is created the way it is. The reasons for author's choices are based on scholarly literature, but also inspired by different fantasy novel translations. There is also a comparison between various typological differences between Czech and English. The result of this bachelor thesis is an attempt to translate an authentic part of a literary work from English to Czech with a focus on aspects which are essential to the translation.
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