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A heterologous expression of alpha-amino acid ester hydrolase from the strain Achromobacter sp. CCM 4824 in Escherichia coli BL21(DE3)
Schneiderová, Michaela ; Kyslík, Pavel (advisor) ; Lichá, Irena (referee)
On the chromosomal DNA of the microorganism Achromobacter sp. CCM 4824, which was gained in the Laboratory of enzyme technology MBU AVCR v.v.i., there was identified a gene coding an enzyme capable of hydrolyzation of semi-synthetic β-lactam antibiotics ampicillin and cephalosporin with a D-phenylglycine as a side chain. This enzyme belongs to a group of α-amino acid esterases, which are interesting because of a potential use in kinetically controled synthesis of β-lactam antibiotics. In several aspects α-amino acid esterases might be better than actually used penicillin acylases and that is why these enzymes are subjects of a big interest. The gene for α-amino acid esterase coded by chromosomal DNA was cloned, characterized and heterologously expressed in constructed highly-producing bacterial system Escherichia coli BL21(DE3)JM5. Produced enzyme was purified and its properties important for possible use in the production of semi-synthetic β-lactam antibiotics were determined. Key words: alpha-amino acid ester hydrolase, Achromobacter sp., recombinant expression system, β-lactam antibiotics
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Dying and death from health care professionals perspective
Klokočková, Šárka ; Schneiderová, Michaela (advisor) ; Votroubková, Michaela (referee) ; Machalová, Iva (referee)
This bachelor thesis analyzes - from a health care professionals perspective-an issue of dying and death. In the theoretical part ,terms concerning dying and death are highlited and described. Attention is also given to needs of dying patients, grief and some ethical questions are raised. Paliative and hospic care is also mentioned. Main component of this thesis is a quantitative research. The research investigates health care professionals opinion on some issues related to care in terminally ill and dying patients. A level of composure in care givers is also being discussed.
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Perioperative care from the point of view of a nurse anaesthetist
Vyskočilová, Jitka ; Schneiderová, Michaela (advisor) ; Dostálková, Šárka (referee) ; Machalová, Iva (referee)
Author: Jitka Vyskočilová Department: Institute of Social Medicine Charles University in Prague Fakulty of Medicine in Hradec Králové. Department of nursing Title of thesis: Perioperative care from the point of view of a nurse anaesthetist Consultant: PhDr. Michaela Schneiderová Number of pages: 85 Number of appendices: 3 Session: 2010 Key words: Anaesthesia, patient, perioperative, nurse, care SUMMARY: Bachelor thesis "Perioperative care from the point of view of a nurse anaesthetist" presents a perioperative period as a part of comprehensive medical care. The theoretical part of the theses introduces a role of a nurse anaesthetist as a participant in perioperative care. There is described required operating theatre equipment as well as preparation of patients and care before, during and after anaesthesia. A focus of the theses is an empirical part based on a quantitative research realized by non- standardized questionnaire research. The research records sequence of care for patients, a level of cooperation and possible risks for patients during a perioperative period. Fifty respondents from nurse anaesthetist community took part in the research in August 2010; 25 of respondents work in Faculty Hospital Královské Vinohrady Prague; 25 of respondents work in Faculty Hospital Brno.
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The importance of dispather-assisted basic life support in the CPR
Holubová, Zuzana ; Schneiderová, Michaela (advisor) ; Mašek, Jiří (referee) ; Kupečková, Ivana (referee)
ANOTATION Zuzana Holubova The importance of dispather-assisted basic life support in the CPR Bachelor thesis deals with the importance of telephone-assisted CPR in the CPR. The theoretical part introduces the koncept of first aid, it is meaning and it is legislation. It focuses on cardiopulmonarz resuscitation in the light of the kontext of the formation and development of life-threatening conditions and introduces the koncept of telephone-assisted CPR and it is important role in providing basic life support. The focus is the empirical part, which is repared on the basis of quantitative research prepared by a questionnaire survey. The sample consists of 80 participants from non-medical public Hradec Kralove region, residing in the district of Nachod. The aim of the research is to map the importace of telephone-assisted CPR in the provision of basic life support.
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Nursing errors
Votroubková, Michaela ; Schneiderová, Michaela (advisor) ; Kutnohorská, Jana (referee) ; Machalová, Iva (referee)
Keywords: nurses mistakes, nurses violence, motivation, nurse, burn out syndrom, relation of nurses to patient, prevent aquisition Evevery of medical institutes are trying to offer the best care of their patients. All of hospitals want to aspire of acreditation and try to find every risks to prevent of patients damage. This problems including nurses mistakes also. In part of theory of my bachelor thesis I tried to explain problems bear on nurses mistakes. I wrote about causes of patients damage, why nurses having bad behaviour to their patiens, which nurses také care about. I tried to propose to prevent aquisition againts nursing violence of patiens. Mistakes of nurses can't be forgot, but every mistakes could be a chance to be better in our work. In part of research I choose research of quality and I used standardized interview. I spoke wit six patiens, who was lying in the hospital and I wrote everything, what they said to me. I asked them about problems of damage of patiens by nurses. I asked of their experiences with this problem. Endly I asked them of prevent aquisition againts nurses violence of patiens.
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Basal stimulation
Novotná, Lenka ; Schneiderová, Michaela (advisor) ; Kupečková, Ivana (referee) ; Juřeníková, Petra (referee)
The paper deals with the sence of a basal stimulation. It's composed of a theoretical part and a practical one. In the theoretical part there is global information about basal stimulation, its components and development. The paper is directed on positioning and the main accent is on the nest position. The influence on psychical and physical perception is included too. Explored group is composed of healthy persons and patients who are suffering from stroke and are hospitalizated on the Neurological clinic. In the practical part there is research that in praxis explores how do people react in the nest position. All of them expressed their feelings by drawing a picture with comentary.
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Education of the diabetics in the origin of possible complications related to diabetes mellitus
Krejčová, Monika ; Schneiderová, Michaela (advisor) ; Knížková, Gabriela (referee) ; Vachková, Eva (referee)
This disertation is concerned to the education of the diabetics about the origin of possible complications that appear in relation with diabetes mellitus. Theoretic passage is oriented to the education which is a necessary component of treatment, to its importance, to goals, phases, form, means of the education, organisation and contents. This passage also approximates the issues of diabetes mellitus complications, such as classification, causation, pathogenesis, clinical picture and therapy of particular complications. The core of this work has been created from a quantitative survey about the knowledge of diabetes mellitus complications, and was made from the diabetics and the common nurses at Regional Náchod Hospital in Broumov. The resultant knowledge should be similar due to educational activities of the nurses. The outcome of this work is a suggestion of the educational plan for diabetics which is oriented to prevention from emergence of diabetic foot.
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