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Analýza chovu masného skotu a způsoby výkrmu v podmínkách ČR
Mácha, Josef
In this bachelor thesis, I deal with the importance of breeding beef cattle or suckler cows, whose breeding in the Czech Republic is still a new industry, even though it operates here for over ten years. It is a system of beef cattle production with efficient use of permanent grassland, budget housing and low labor costs. The second chapter focuses on the current numbers of beef cattle breeds reared in the Czech Republic, which will be followed by the third chapter, which will be engaged in the distribution and description of selected beef cattle. In the second part of this work is described a system of rearing cows, beef cattle breeds, and the last chapter focuses on the fattening of the beef cattle.
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Purification of recombinant proteins by affinity chromatography
Zemek, Ondřej ; Mácha, Jaroslav (advisor) ; Hrdý, Ivan (referee)
The isolation and purification of recombinant proteins is essential for further study of their structural and functional properties. The affinity chromatography is usually the method of choice for this task. In this paper the most used affinity tags are reviewed for their properties and experience with their application. The tags covered here include CBP, MBP, GST, polyhistidine and polyarginine tags, FLAG-tag, Strep-tag II and SpA. The origin and properties of the tags, their influence on form and localization of fusion protein as well as binding, elution and removal are discussed. Keywords: affinity chromatography, recombinant protein, purification, affinity tag
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The role of Src family kinases in RNA processing
Gemperle, Jakub ; Rösel, Daniel (advisor) ; Mácha, Jaroslav (referee)
Until now, a lot of information have been obtained about the role of Src family kinases in the cytoplasm or at the plasma membrane and their interactions with growth factor receptors or focal adhesion complexes. Their functional importance at the perinuclear membrane, or even inside the nucleus, however, has not been well characterized. This work, using available information, pointed at the fact that Src family kinases can be found in the nucleus. This opens a new field of Src kinases action, such as in RNA metabolism, considering that it has been assumed that their activity is limited to the cytoplasmic compartment. This work summarizes the current knowledge that hints to Src family kinases dependent network of regulation of RNA metabolism; Src family kinases have pleiotropic effects not only on the RNA binding proteins, but also on the remodeling of chromatin structure. These kinases affect by direct interactions with other proteins transport, splicing or RNA stability and gene expression. This summary suggests that Src family kinases could regulate RNA metabolism on many levels.
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