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Functional analysis of differentially expressed miRNAs in HPV positive and HPV negative cell lines derived various anatomical localization
Krbušek, David ; Saláková, Martina (advisor) ; Dostálová Merkerová, Michaela (referee)
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. MiRNA expression profiles showed several differentially expressed miRNAs specific to human papillomavirus (HPV)-associated tumors. These miRNAs include miR-139-5p, which has reduced expression in these tumors. In this thesis, the role of miR-139-5p was studied in vitro on cell lines that were HPV positive (CRL-3240, SiHa) and HPV negative (FaDu, C-33A). Cell lines were transfected with mimic miRNA, the ability of cells to proliferate and migrate was then studied. Cell proliferation was studied using MTT assay, while Scratch wound healing assay and transwell assay were used to evaluate the migratory abilities of the cells. Mediator RNAs (mRNAs) of target genes of miR-139-5p were predicted using TargetScan and miRDB databases. The change in gene expression of target mRNAs, as well as the verification of the successful increase in miRNA expression in the cell lines, was verified using RT-qPCR. Increase of miR-139-5p expression in all used cell lines did not lead to statistically significant changes (p≤0.05) in proliferative or migratory abilities. The mRNAs of FOS, JUN, KIF13A and GDF10 genes were selected as targets of miR-139-5p. Transfected cell lines did not show a noticable reduction in the expression of the target mRNAs....
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Adult neurogenesis in the hippocampus and spatial navigation
Dostálová, Magdalena ; Stuchlík, Aleš (advisor) ; Jiruška, Přemysl (referee)
Hundreds to thousands of new granule neurons are born every day in the hippocampus within the whole life. Inspite of intensive research of the last decades, their function remains unclear. Their participation in spatial memory and the ability of orientation is assumed. Results from behavioral tests like the Morris water maze, the radial maze and many other testing tasks, are often contradictory. Nevertheless, it is highly probable that neurogenesis plays a role in pattern separation and long-term relational memory. Further studies and especially reliable methods of ablation and detection are required for deeper insight into this issue.
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Twistor operator in symplectic spin geometry
Dostálová, Marie ; Krýsl, Svatopluk (advisor) ; Doubek, Martin (referee)
The topic of the diploma thesis is symplectic spinor geometry. Its re- search was started by D. Shale, B. Kostant and K. Habermann. We focus our attention to one of the so called symplectic twistor operators introduced by S. Kr'ysl. We investigate the action of this operator on real even dimensio- nal vector spaces considered as symplectic manifold, its invariance properties and regularity. We describe a part of the kernel of the symplectic twistor operator when acting on symplectic spinors on R2. The kernel forms a repre- sentation of the so called metaplectic group (double cover of the symplectic group). 1
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The role of microRNA in physiology and pathology
Janáková, Barbora ; Matoušková, Petra (advisor) ; Pávek, Petr (referee) ; Dostálová Merkerová, Michaela (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Barbora Hanousková Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of Doctoral Thesis: The role of microRNA in physiology and pathology MicroRNAs (miRNAs) are small single-stranded non-coding RNA molecules that play an important role in the regulation of gene expression. They are evolutionarily highly conserved and are present in the genome of all eukaryotic organisms, suggesting their importance in physiological processes. Due to the tissue specificity and their involvement in the pathogenesis of various diseases, miRNAs have been widely studied in connection with their potential use as specific and early biomarkers, or as a therapeutic target. The doctoral thesis, written in the form of an annotated set of publications, dealt with the study of miRNAs in various systems in vitro and in vivo, focusing on their role in adipogenesis and their use as biomarkers of pathological conditions. The introduction summarizes the theoretical information on epigenetic regulation with a more detailed focus on miRNAs, followed by comments on the author's individual publications and brief conclusions. In two studies using mouse models, several miRNAs were identified to have their expression profile...
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The role of microRNA in physiology and pathology
Janáková, Barbora ; Matoušková, Petra (advisor) ; Pávek, Petr (referee) ; Dostálová Merkerová, Michaela (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Barbora Hanousková Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of Doctoral Thesis: The role of microRNA in physiology and pathology MicroRNAs (miRNAs) are small single-stranded non-coding RNA molecules that play an important role in the regulation of gene expression. They are evolutionarily highly conserved and are present in the genome of all eukaryotic organisms, suggesting their importance in physiological processes. Due to the tissue specificity and their involvement in the pathogenesis of various diseases, miRNAs have been widely studied in connection with their potential use as specific and early biomarkers, or as a therapeutic target. The doctoral thesis, written in the form of an annotated set of publications, dealt with the study of miRNAs in various systems in vitro and in vivo, focusing on their role in adipogenesis and their use as biomarkers of pathological conditions. The introduction summarizes the theoretical information on epigenetic regulation with a more detailed focus on miRNAs, followed by comments on the author's individual publications and brief conclusions. In two studies using mouse models, several miRNAs were identified to have their expression profile...
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Small noncoding RNAs and their prognostic value in myelodysplastic syndromes
Hruštincová, Andrea ; Dostálová Merkerová, Michaela (advisor) ; Stopka, Tomáš (referee) ; Vymetálková, Veronika (referee)
Myelodysplastic syndromes (MDS) are a heterogeneous group of bone marrow (BM) disorders characterized by ineffective haematopoiesis, BM dysplasia, and peripheral blood cytopenia. In recent years, substantial progress has been made towards understanding the molecular pathogenesis of MDS that has brought new possibilities in MDS diagnostics, prognostics, and treatment. Small noncoding RNAs (sncRNAs), especially microRNAs (miRNAs), are in the field of scientific interest in terms of their expression, function, role in disease development, and potential utilization as disease biomarkers. Special attention has been focused on extracellular sncRNAs present in blood circulation, so called 'circulating' sncRNAs, which may become easily accessible biomarkers of disease state or risk of progression. We have conducted several studies on intracellular and extracellular sncRNA profiles of CD34+ BM cells and blood plasma, respectively, from MDS patients using microarrays or next generation sequencing (NGS). We aimed to identify specific sncRNA profiles associated with MDS and search for sncRNA biomarkers predictive of the patient prognosis and response to treatment with azacitidine (AZA). Another goal was to characterize and compare circulating sncRNA profiles of two different extracellular materials, total...
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Rehabilitation care system for children with leukaemia in Czech Republic and selected countries of Europe
Dostálová, Markéta ; Jevič, Filip (advisor) ; Ptáková, Karolína (referee)
Leukemia is the most common cancer in childhood, making up 30-35% of all oncological diseases in children in the Czech Republic. The most frequent form of leukemia in pediatric patients is acute lymphoblastic leukemia (ALL). This form accounts for 80% of leukemia in children. Other types include acute myeloblastic leukemia (AML), myelodysplastic syndrome (MDS), and chronic myeloid leukemia (CML). Treatment is based on the type and subtype of leukemia diagnosed and usually lasts 1-2 years, provided there is no relapse. Primary treatment involves many side effects, including on the musculoskeletal system, such as chemotherapy-induced polyneuropathy (CIPN) or muscle myopathy as an adverse effect of glucocorticoid therapy. Part of the comprehensive treatment is supportive treatment, which includes rehabilitation (RHB). The aim of this work is to find out and compare whether and under what conditions RHB in pediatric haemato-colongic patients is in the Czech Republic and selected countries of Europe, who and according to what patients RHB and whether clinics have any programs related to RHB in these patients. The general part introduces the issue of pediatric patients with leukemia including the possibilities and adverse effects of treatment. The special part is devoted to research based on...
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Zhodnocení současného stavu a péče o vybraná zvláště chráněná území okresu Hodonín
Dostálová, Martina
In this bachelor work was evaluated 6 small specially protected areas (SPA) in the district Hodonin a total area of 108.15 hectares. There is detailed disassembly present state and management in every one protected areas and interpretation results were evaluated with the assistance of a methodology designed to test the SPA (Svátek, Buček 2005). Using this methodology, we get a quick and efficient overview of the current status and management of protected areas. The work is composed in such a way that it first introduced the selected area and its natural conditions and followed his evaluation.
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Monitoring mokřadního biotopu u obce Podolí (býv. okr. Uherské Hradiště) tři roky po založení
Dostálová, Martina
The aim of the work theses was to evaluate the actual state of vegetation in the wetland biotope near the village of Podolí (former district Uherské Hradiště) three years after its establishment. Evaluation of vegetation and its entry into floristic inventories and classification according to the Biotope Catalog (Chytrý et al., 2010). Furthermore, the current status of sub-areas in the locality was evaluated according to 4 criteria for which a simple relative scale was created. The results showed that according to the Biotope Catalog (Chytrý et al., 2010) there are areas that can be classified into five different habitats. The prevailing habitat X 7 (herbaceous ruderal vegetation outside human settlement) where habitat types are not predominant. These are mostly ruderal and synanthropic species The evaluation criteria showed where, at the time of the mapping, there was a water surface or waterlogging, what was the nature of the vegetation, whether the management was suitable or not, and finally whether the individual areas perform the function for which they were built. There should be some changes to management measures and leave room for succession.
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Technologické vlastnosti křepelčích vajec
Dostálová, Marie
Technological properties, in other words functional properties, of quail eggs influence the preparation and production of food. The basic functional properties of egg albumen include gel formation, foam formation and suppression of crystallization. Gel formation is important in the production of boiled quail eggs. Foam formation is necessary in the production of confectionery and bakery products. Suppression of crystallization is used especially for confectionery. The functional properties of egg yolk can include the emulsion formation and antioxidant action of the phospholipids of yolk. Emulsion formation is important in mayonnaise production. For certain technological properties, there are defined criteria, which describes applicability and quality of eggs. Foam ex-pansion index, foam stability index, foam density and toughness are obtained during the foam formation of quail fresh or dried albumen. Emulsion stability index and emulsion activity index are obtained during formation of emulsion from quail fresh or dried yolk. Functional properties are influence affected for example by solubility of protein, water-holding and oil-holding capacity.
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