Malé nekódující RNA a jejich prognostický význam u myelodysplastického syndromu

Hruštincová, Andrea

Myelodysplastic syndromes (MDS) are a heterogeneous group of bone marrow (BM) disorders characterized by ineffective haematopoiesis, BM dysplasia, and peripheral blood cytopenia. In recent years, substantial progress has been made towards understanding the molecular pathogenesis of MDS that has brought new possibilities in MDS diagnostics, prognostics, and treatment. Small noncoding RNAs (sncRNAs), especially microRNAs (miRNAs), are in the field of scientific interest in terms of their expression, function, role in disease development, and potential utilization as disease biomarkers. Special attention has been focused on extracellular sncRNAs present in blood circulation, so called 'circulating' sncRNAs, which may become easily accessible biomarkers of disease state or risk of progression. We have conducted several studies on intracellular and extracellular sncRNA profiles of CD34+ BM cells and blood plasma, respectively, from MDS patients using microarrays or next generation sequencing (NGS). We aimed to identify specific sncRNA profiles associated with MDS and search for sncRNA biomarkers predictive of the patient prognosis and response to treatment with azacitidine (AZA). Another goal was to characterize and compare circulating sncRNA profiles of two different extracellular materials, total...

guest :: login Digital Repository
		Search		Submit		Help		About