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Teplá, O. ; Pěknicová, Jana ; Mrázek, M. ; Kočí, K. ; Chládek, David ; Míka, M. ; Badalová, J. ; Pecka, T. ; Fulka, F.
Iscare IVF a.s. had a long record of successful azoospermic treatment since 1997, when the first pregnancies by MESA a TESE were published in the Czech republic. A series of diferent implanted laboratory techniques supported good results from last year. The medical problem is solved during the other field of application-histlogical findings and immunofluorescent methods.
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Antitrophoblast cell-mediated immunity /AT-CMI/ in recurrent miscarriage and ectopic pregnacy
Madar, J. ; Kučera, E. ; Šůla, K. ; Chaloupková, A. ; Pěknicová, Jana ; Tolarová, M. ; Nouza, K. ; Kinský, R.
Specific mechanisms operate in decidua nad trophoblast to establish and maintain immunological tolerance of the embryo in mother's uterus. due to the lack of this tolerance, disturbances of pregnancy may result. In this presentation, we have tested the hypothesis that anti-trohhoblast cell-mediated immunity (AT-CMI) is elevated in habitually aborting women and that extra-uterine localisation of the embryo leads to subsequent elevation of AT-CMI.
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Characterization of peptide proteinase inhibitors isolated from boar seminal plasma
Jelínková, Petra ; Tichá, M. ; Jonáková, Věra
Most of proteins of boar seminal plasma are present in this medium under physiological conditions in the form of aggregates and not of monomers. In the present communication we have shown that not only spermadhesins, but also proteinase inhibitors participate in the formation of associated complexes. Proteinase inhibitors were found after gel chromatographic separation mainly in the fraction with the lowest relative molecular mass. Two different types of serine proteinase inhibitors were isolated from boar seminal plasma ů (i) glycosylated inhibitor with Mr 12 kDa, (ii) non-glycosylated sperm-associated acrosin inhibitor with Mr 8 kDa, and a new type of low molecular weight protein that is probably composed of two subunits coupled by the -S-S- bridge. The proteinase inhibitor with Mr 8 kDa was proved to form a heterodimer with AQN 1 spermadhesin under physiological conditions, whereas inhibitor with Mr 12 kDa forms homodimers.
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Validation of macromolecular crystal structures
Řezáčová, Pavlína
X-ray crystallography is the main technique of macromolecular structure determination. Typical problems of protein crystallography diffraction data measured at limited resolution and inaccurate phase informacion adversely affect the quality of electron density maps and consequently the quality of the final model. Currently, there are many methods of protein quality validation. These can be subdivided into two main groups The first group assesses the model quality by evaluating the agreement of the model with the diffraction data. The second group of structure validation methods is based on comparison of the macromolecular model geometrical parameters with standards values derived from the crystal structures of small molecules or high-resolution macromolecular structures. This work is focused on introduction to widely used macromolecular structure-validation software. The main global quality criteria and reliable indicators for detecting local errors are summarized.
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