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Design and construction of the prototype of universal semi-automatic equipment for the automotive industry
Kristel, Peter ; Slaný, Martin (referee) ; Sedlák, Josef (advisor)
Construction of semi-automatic equipment DCR 231 means for company Mubea saving money in long-range target. This economy profit rises with time period of the equipment active usage. The equipment is designed as a universal tool of automatic production step for inserting reinforcement tubes into tubular stabilizer bars. Inserting of these tubes into the both stabilizer ends brings higher strength of supported areas. Benefit of stabilizer bar reinforcement only on its ends is a lower mass leads to reduction of the whole axle mass. The equipment in global scale helps to reduce the costs connected with daily vehicle usage.
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Possibility of determination of selected flavonoides
Smělá, Margita ; Vitoulová, Eva (referee) ; Vespalcová, Milena (advisor)
The theoretic part of the bachelor‘s thesis deals with description of selected flavonoids as apigenin, quercetin, luteolin, keampferol, rutin, myricetin and with options of their determinations. There were described typical properities and effects of several flavonoids at first. In the next part there are principles of methods, especially high-performance liquid chromatography and capillary electrophoresis. The biggest attention was made to literary survey of determination flavonoids coumpounds mentioned above, by high-performance liquid chromatography and capillary electroproresis. There were described the collection of plant material for extraction of flavonids in the practical part. The white and blue grapes were frozen by dry ice as soon as they were separated from a busch. Grapes were made in a nitrogenous atmosphere and lyophilizated after the frozen.
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Posttranslational modification of proteins - their analysis and physiological aspects
Mikulíková, Kateřina ; Mikšík, Ivan (advisor) ; Bílková, Zuzana (referee) ; Glatz, Zdeněk (referee)
This thesi s is eoneerned with the deteetion of posttranslational modifieation of proteins. These proteins are identified using liquid ehromatography and eapillary eleetrophoresis, their eoupling and eoupling to mass speetrometry. The first part of the theoretieal ehapter is devoted to the strueture, classifieation and metabolits ehanges of proteins in the human body. Major attention is foeused on eollagen, its strueture and individual eollagen types. The seeond part eonsiders the problems of nonenzymatie glyeation and its influenee on the body and engage in produets of posttranslational modifieation. The third part summarizes the individual separation proeedures and analysis of posttranslationaly modified proteins. The seeond ehapter deseribes experimental parameters, including ehemieals, instrumentation, materials and methods. The preparation of individual samples is also deseribed. All results and findings are summarized and diseussed in the last ehapter. This ehapter is divided in two parts. The first part foeuses on problems of in vivo experiments. The seeond part foeuses on problems of in vitro experiments. Powered by TCPDF (www.tcpdf.org)
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Analysis of biologically active substances by modern separation methods
Bierhanzl, Václav ; Čabala, Radomír (advisor) ; Pacáková, Věra (referee) ; Sýkora, David (referee)
The thesis is dedicated to the phospholipids and their polar headgroups analysis by gas chromatography, capillary electrophoresis and mass spectrometry. Phospholipids are the most important polar lipids and they are classified into phospholipid classes according to their phosphorylated groups. Phospholipids can be found in cell membranes and the changes in their ratio are monitored to research the impact of external conditions on cells. Actually thin layer chromatography is still used for phospholipid class ratio analyses. It is not suitable for microbiological research due to its time demandingness. The presented compendium of papers engaged in phospholipid classification is targeted on Bacillus subtilis strain, which produces potential antibiotics with detergent effect - surfactin. Published methods can be used for research of optimal conditions for producing microbe cultivation. Because non-polar parts of the phospholipid molecule (fatty acids) can affect the analysis methods on spliced polar headgroups have to be designed. Capillary electrophoresis and gas chromatography methods were developed and the latter one was further optimized for simultaneous analysis with fatty acids. Additional part deals with an alternative approach which consists in direct injection on mass spectrometer of intact...
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Preparation and optical properties of scintillation oxide layers
Hanuš, Martin ; Kučera, Miroslav (advisor) ; Čuba, Václav (referee) ; Pejchal, Jan (referee)
In this work we studied properties of garnet scintillator layers (RxLu3-xAl5O12, RxY3-xAl5O12) doped by rare earth ions (Ce, Pr, Tb), orthosilicates (Y2SiO5; R = Ce, Tb) and influence of Sc codoping on Pr3+ and Tb3+ emissions. The Zr codoping on Ce3+ emission in orthosilicates was also studied. The samples were prepared by liquid phase epitaxy. The studied materials show high quantum efficiency and good chemical and mechanical stability. They represent ideal materials for 2D imaging devices. We studied optical absorption, excitation and emission spectra and scintillation properties (radiolunescence and photoelectron yield). The aim was to determine the properties of grown layers and their comparison to Czochralski grown single crystals. We looked for the impact of melt and growth conditions on measured layer properties. We also tried to determine optimal amount of dopants in layer. We used PbO - B2O3 and BaO - BaF2 - B2O3 fluxes. Using these fluxes, we succeeded in growing layers with less intrinsic defects in crystal lattice in comparison to single crystals. In grown layers of thickness from 1 to 30 µm higher dopant concentration was achieved than in single crystals.
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Preparation and optical properties of scintillation oxide layers
Hanuš, Martin ; Kučera, Miroslav (advisor)
In this work we studied properties of garnet scintillator layers (RxLu3-xAl5O12, RxY3-xAl5O12) doped by rare earth ions (Ce, Pr, Tb), orthosilicates (Y2SiO5; R = Ce, Tb) and influence of Sc codoping on Pr3+ and Tb3+ emissions. The Zr codoping on Ce3+ emission in orthosilicates was also studied. The samples were prepared by liquid phase epitaxy. The studied materials show high quantum efficiency and good chemical and mechanical stability. They represent ideal materials for 2D imaging devices. We studied optical absorption, excitation and emission spectra and scintillation properties (radiolunescence and photoelectron yield). The aim was to determine the properties of grown layers and their comparison to Czochralski grown single crystals. We looked for the impact of melt and growth conditions on measured layer properties. We also tried to determine optimal amount of dopants in layer. We used PbO - B2O3 and BaO - BaF2 - B2O3 fluxes. Using these fluxes, we succeeded in growing layers with less intrinsic defects in crystal lattice in comparison to single crystals. In grown layers of thickness from 1 to 30 µm higher dopant concentration was achieved than in single crystals.
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The role of molecular genetic and cytogenetic analyses in the diagnosis and prediction of treatment response in patients with non-Hodgkin lymphomas
Berková, Adéla ; Zemanová, Zuzana (advisor) ; Goetz, Petr (referee) ; Smolej, Lukáš (referee)
Malignant lymphoproliferative disorders include highly heterogeneous entities, i.e. lymphomas (Non-Hodgkin - NHL, as well Hodgkin's lymphoma), lymphoid leukemias, multiple myeloma and others. As currently many chromosomal aberrations with diagnostic and prognostic significance are known, molecular cytogenetic analyses of tumor cell genome has become a substantial examination also in lymphoproliferative disorders. This thesis focuses primarily on chronic lymphocytic leukemia (CLL), which is one of the mature B-cell neoplasms and represents the most common type of leukemia. We analyzed four most frequently found aberrations (13q14 deletion, ATM and TP53 gene deletion, and trisomy 12) by fluorescence in situ hybridization (FISH) and also IgH gene aberrations in some patients. We compared the findings with other factors and clinical characteristics. This work shows that the conventional G-banding is analysis relatively little relevant. FISH was more effective in detecting aberrations in CLL. Although none of the four aforementioned changes is specific to CLL, the prognostic impact is significant, particularly that of TP53 deletion. Next, detection of some IgH gene translocations is essential in differential diagnosis of CLL and other NHL (follicular, mantle cell, diffuse large B cell, Burkitt's...
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Analysis of biologically active substances by modern separation methods
Bierhanzl, Václav ; Čabala, Radomír (advisor) ; Pacáková, Věra (referee) ; Sýkora, David (referee)
The thesis is dedicated to the phospholipids and their polar headgroups analysis by gas chromatography, capillary electrophoresis and mass spectrometry. Phospholipids are the most important polar lipids and they are classified into phospholipid classes according to their phosphorylated groups. Phospholipids can be found in cell membranes and the changes in their ratio are monitored to research the impact of external conditions on cells. Actually thin layer chromatography is still used for phospholipid class ratio analyses. It is not suitable for microbiological research due to its time demandingness. The presented compendium of papers engaged in phospholipid classification is targeted on Bacillus subtilis strain, which produces potential antibiotics with detergent effect - surfactin. Published methods can be used for research of optimal conditions for producing microbe cultivation. Because non-polar parts of the phospholipid molecule (fatty acids) can affect the analysis methods on spliced polar headgroups have to be designed. Capillary electrophoresis and gas chromatography methods were developed and the latter one was further optimized for simultaneous analysis with fatty acids. Additional part deals with an alternative approach which consists in direct injection on mass spectrometer of intact...
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Elusive view: Ideas about the family and best interests of the child in the context of putting children to alternative family care/education
Jílková, Tereza ; Kolářová, Kateřina (advisor) ; Benešová, Jana (referee)
A subject of this diploma paper is to follow practise of putting children into alternative family care. The key method of finding answers is analysis of interviews with social workers and a worker of socially legal protection of children, and content analysis of texts and documents connected with the issue of alternative family care. The paper examines the role of social workers in the process of putting applicants into register for any form of alternative family care and workers' influence in forming such a process. The aim is to focus on social workers' activity analysis and to discover in which moments and in which ways ideological and normative settings of social workers might affect practice of putting children into alternative family care. One of the points of activity analysis is description of social workers' fulfilment of a concept - the best interest of a child and family. The paper is looking for the answer to what extent and in which moments normative ideas of family and topics family related might affect putting or not putting a child into a particular applicant's care.
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