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Development of natural cosmetics emulsions with encapsulated active substances
Chrápavá, Edita ; Dzurendová, Simona (referee) ; Márová, Ivana (advisor)
In recent years, there is a increase of consumer interest in products, which in some way help a more favourable ecological sustainability, whether it is a reduction of emissions during mass production, easier breakdown of chemicals in wastewater, or harmlessness of products and ingredients from which products are made. This topic also affected the cosmetic industry in a big way, and they were thus forced to start adapting not only smaller brands of natural cosmetics, but also large companies. As a result, bigger companies started to pay more attention to research of natural substances for cosmetics and the development of natural alternatives to synthetic ingredients. At the same time, there is also increasing interest in the effects of Eastern medicine mushrooms and their beneficial effects on both physical and mental health. This thesis focuses on the connection of these two topics, the analysis of plant extracts and extracts of medicinal mushrooms on one side and their potential cosmetic use on the other. Subsequently, a cosmetic product was designed made from ingredients of natural origin with encapsulated extracts from these natural ingredients as active substances
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Development of cosmetic products with antimicrobial effect
Dribňáková, Barbora ; Wikarská, Monika (referee) ; Márová, Ivana (advisor)
The proposed bachelor's thesis focuses on the preparation and study of nanomaterials with antimicrobial properties. The nanofibers were synthesized from biopolymers using the forcespinning method, and liposomes were prepared using the sonification method. Active ingredients used in this work are tymol, carvacrol, and eugenol. The theoretical section of the thesis discusses the methods for preparing nanofibers and nanoparticles and the materials required for their synthesis. It also covers the encapsulation of active ingredients in nanomaterials. Additionally, this part explores the skin microbiome, antimicrobial effects, and the use of both natural and synthetic antimicrobial agents. The practical part of the thesis focuses on determining the polyphenol content and antioxidant activity of each active ingredient. The gradual release of active ingredients into saline solution and distilled water was determined for nanofibers synthesized from polyhydroxybutyrate with encapsulated active ingredients. Furthermore, it deals with the optimization of nanofiber synthesis from chitosan and polyamide using the forcespinning method. The functionalized liposomes were characterized in terms of their size, stability, encapsulation efficiency, and gradual release of active ingredients. The liposomes showed high stability even after several weeks, and their size ranged from 170.90 ± 1.63 nm to 243.07 ± 7.83 nm. Finally, the antimicrobial activity of the prepared products and active ingredients was tested against Cutibacterium acnes, Staphylococcus epidermidis, and Escherichia coli. The tested active ingredients in pure and encapsulated form, demonstrated antimicrobial effect against both gram-positive and gram-negative bacteria. We assume the possibility of using the prepared nanomaterials in cosmetic products intended for the treatment of acne and other skin diseases, and also its use in the form of preservatives.
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Optimization of Combined Chitosan Particles and their Characterization for Application Use
Netopilík, Tibor ; Hudečková, Helena (referee) ; Márová, Ivana (advisor)
The thesis deals with the preparation and characterization of nanoparticles for cosmetic and food applications. The thesis explores various methods of preparing nanoparticles, including liposomes and chitosomes, and analyses their properties and stability in different model environments. The experimental part includes the determination of the encapsulation efficiency of different vitamins by HPLC and UV-VIS spectrophotometry. Results show the influence of various factors such as time, type of environment and method of preparation on stability and efficiency of the nanoparticles. Furthermore, the optimization of the preparation is carried out chitosomes using ultrasound and magnetic stirrer. Cosmetic products enriched with these nanoparticles were developed and their sensory analysis was proved. This work contributes to the understanding of nanoparticle preparation processes and provides useful information for the development of new cosmetic and food products with improved properties and stability.
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Production of microbial enzymes and their stabilization by encapsulation
Hazuchová, Eva ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
The present thesis deals with the production of microbial enzymes and their subsequent stabilization through encapsulation. The theoretical part focuses on microbial enzymes, especially extracellular hydrolases, their producers and characteristics. Within the theory is also discussed the possibility of the application of enzymes in the field of pharmacy and medicine. Experimental work was focused on the actual production of microbial enzymes and methods for their to stabilization. The production of proteolytic and lipolytic enzymes in dependence on time and the used culture substrate were followed. The highest enzyme production was observed in Aspergillus oryzae when cultured on wheat bran at the third day of cultivation. In the experimental part was further carried out the identification, isolation and purification of enzymes. A substantial part of the experiment was to stabilize produced microbial enzymes by encapsulation. Enzymes were entrapped into alginate particles with encapsulation efficiency in the range of 55-70 %. The highest efficiency exhibited encapsulated enzymes from Aspergillus oryzae. Subsequently, long-term stability of the encapsulated enzyme in two environments (in water and gel) was followed during six weeks incomparison with free enzyme. During storage of free enzyme a significant decrease in enzyme activities occured, especially between the fourth and sixth week of storage. On the contrary, in encapsulated increased enzyme activities were observed. Empty particles exhibited higher stability during storage in the gel than in water. In this thesis potential use of enzymes in the pharmaceutical industry as agents promoting digestion was tested too. According to the results, particles with encapsulated microbial enzymes could be considered as suitable for some pharmaceutical applications.
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Equipment for sports and relaxation
Doupal, Ondřej ; Bláhová, Pavla (referee) ; Čuprová, Danuše (advisor)
The project design processes multifunctional center for leisure and sports. The center is situated in Šternberk and its capacity is about 150 people. There is inter alia the wellness center, with saunas and whirlpool, a fitness, gym and a beauty salon. Architecturally, the object is conceived as paralell blending of three units of a different colors and sizes. As well as all construction compositions are more developed basis for air conditioning systems. Documentation is processed at the level of documentation for building permit.
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Isolation of active compounds from algae and their application in cosmetic products
Nováková, Laura ; Byrtusová, Dana (referee) ; Skoumalová, Petra (advisor)
The bachelor thesis is dedicated to the study of active substances and antioxidant effects of cultivated microalgae. We compared the results with a commercial alga that is freely available. In the theoretical part we focused on description of algal metabolites in cosmetics and a brief description of the methods used in their testing. In the experimental part there were analyzed three types of extracts (aqueous, alcohol and oil) from 4 species of algae. It is mainly the content of polyphenols, flavonoids and antioxidant activity that is determined. According to the results, the highest content of active substances was found in oil extracts. All extracts were encapsulated into liposomes. The average size of the nanoparticles was in range of 200 nm to 300 nm. The particles were stable enough also for long-term stability. The largest decrease was observed in the oil extract of the cultivated algae Chlorella vulgaris. The cytotoxicity of oil extracts was realized by MTT test on human cell cultures HaCaT. Finally, we concluded that the prepared liposomal particles could be used for anti-aging cosmetics.
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Aronia as a raw material for cosmetic production
Palkovská, Kateřina ; Sýkora, Michal (referee) ; Zemanová, Jana (advisor)
This bachelor thesis deals with extraction of polyphenols from fruits and leaves of aronia and consequent use of obtained extract in cosmetic production. In the theoretical part are information about the origin of the aronia, the botanical characteristics of Aronia Melanocarpa and about its utilization. Furthermore, the chemical composition of the plant material of the aronia is described, which is also common for fruits. At the end of the theoretical part are described extraction and separation techniques, methods of determination of total polyphenolic compounds and brief characteristics of the material safety data sheet. The experimental part describes the optimization of the prepared extract so that the extract contains as many polyphenolic compounds as possible. 50% ethanol at room temperature was shown to be optimal for leaf and fruit extraction, and the maceration time was found to have a significant effect on the polyphenolic compounds obtained. For the obtained optimized macerate, some chemical and physical properties such as pH, density, refractive index were determined for the purpose of producing a material safety data sheet and product specification. At the end of the experimental part were prepared cosmetic products with the addition of macerate from crushed aronia fruits.
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Study of particles of various cosmetic pigments
Cerevatova, Kristina ; Opravil, Tomáš (referee) ; Zemanová, Jana (advisor)
The main focus areas of this master thesis include the writing of a literature review, which contains an overview and segregation of pigments which are used in the preparation of cosmetic products. The thesis is focused on the properties and characterization of pigments for use in cosmetology. The following focus is on legislation, more specifically on pigments that the cosmetic industry allows, limits and does not allow. The main part of this thesis is an overview of methods for the characterization of pigments. The practical part deals with the characterization of particles of selected cosmetic pigments by applying methods for determination of color, elemental and phase composition, particle size and morphology. The thesis is concluded by evaluation of the achieved results.
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Study of preservative systems for cosmetics
Šmétková, Zuzana ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
The aim of this diploma thesis was to prove the antimicrobial actvivity of the selected syntetic preservatives used in cosmetics, such as methylparaben, butylparaben, phenoxyethanol, bronopol, imidazolidinyl urea and methylisothaizolinone, in different concentrations (up to the highest permitted concentration and in increased concentrations), and of some essential oils, which are possible to use in cosmetics (essential oil from Eucalyptus globulus, Pimpinella anisum, Lavandula officinalis, Rosmarinus officinalis, Foeniculum vulgare, Mentha piperita, Juniperus communis and Pinus sylvestris). The effect was investigated in every preservative system alone, in order to evaluate the spectrum and the rate of each one. As indicator microorganism was chosen Bacillus subtilis and Penicillium chrysogenum. The used methods was disk difusion method and modify difusion method. From synthetic preservatives had the best effects bronopol, imidazolidinyl urea and methylisothiazolinone. From chosen natural antimicrobails had the effect against both indicator microorganisms the Mentha piperita, Foeniculum vulgare and Juniperus communis extracts.
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Analysis of aroma compounds in cosmetics by gas chromatography
Divišová, Radka ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
This thesis is focused on aroma compounds which are presented in a wide variety of products including perfume, cologne, cosmetics, toiletries, laundry products and detergens. The widespread use and exposure to aroma compounds of aforementioned products could cause a range of the adverse reactions such as contact dermatitis, asthma, eczema and breathing problems. There are a lot of aroma compounds triggering negative allergic reactions but EU regulations limit 26 the most important fragrance allergens. The presence of these substances has to be declared on the product label if a limit of 0,01 % for rinse off and 0,001 % for leave-on products is exceeded. Of these 26 substances, a methodology was elaborated for determination of 12 allergens in a experimental part of this thesis. A method by solid-phase microextraction (SPME) and gas chromatography-flame ionization detection (GC-FID) was applied for fragrance allergens identification, extraction and measurement. The optimal extraction conditions of SPME-GC method were investigated including equilibrium time, extraction time and extraction temperature to increase extraction efficiency. Another important point is the method by SPME-GC-FID was validated and following performance parameters were determined: repeatability, linearity, limit of detection (LOD) limit of quantitation (LOQ). SPME-GC-FID was tested and applied to real samples.
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