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Rastrovací elektronová mikroskopie mokrých vzorků syntetických polymerů
Šlouf, Miroslav ; Studenovská, Hana ; Rypáček, František ; Sikora, Antonín
Synthetic polymers with high content of water (wet synthetic polymers,hydrogels) are frequently used in tissue engineering. Investigation of their 3D-structure by means of scanning electron microscopy is very difficult: in a standard electron microscope water almost immediately evaporates and the structure breaks down. Nevertheless, hydrogels morphology can be visualised by means of low-vacuum scanning electron microscopy in cryo conditions (cryoLVSEM) and by environmental scanning electron microscopy (ESEM). The two techniques are described and compared.
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3D Construction View of Differential Pumping Chamber with Detector Board of Environmental Scanning Electron Microscope
Neděla, Vilém
This paper deals with construction of the differential pumping chamber with a detector board for adaptation of the scanning electron microscope (SEM) to the environmental scanning electron microscope (ESEM). ESEM allows work at higher pressure in some parts of the microscope. It focuses especially on the 3D construction view of the differential pumping chamber together with the detector board of the differential pumping chamber and it comments reasons of necessity of the assembly of these parts into the environmental scanning electron microscope.
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Měření a nastavování vlhkosti v Environmnetálním rastrovacím elektronovém mikroskopu
Autrata, Rudolf ; Jirák, Josef ; Neděla, Vilém ; Špinka, J.
When we are detecting signal electrons with an ionization detector in ESEM, the most advantageous is to use, with respect to the effectiveness of ionization, environment of water vapours in the specimen chamber. The most suitable are saturated water vapours (relative humidity of 100%). This environment is also advantageous for observation of specimens containing water. At pumping of the specimen chamber to the working pressure (typically around 1000 Pa), in the case of wet specimens, evaporation of specimen can occur, or on the contrary, condensation of water on them. It means, that in some cases the specimen will not remain in the original state. That is why we try to adjust and optimize the pumping procedure and pumping of water vapors to minimize deformation of the observed specimens
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