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Evolution of olfactory receptors
Klempt, Petr ; Stopka, Pavel (advisor) ; Vinkler, Michal (referee)
This bachelor thesis deals with the largest gene family of mammals which encode olfactory receptors. Olfactory receptors fall in rhodopsin-like GPCRs subfamily, approximately 600 - 800 millions of years old. At least from this time, olfactory receptors play, as a part of one of the oldest senses (smell), fundamental role in detection of chemical cues from water or air. This work summarizes large repertoire of olfactory receptors and its changes during the evolution of important animal taxons with emphasis on number and fraction of functional and nonfunctional olfactory receptor genes. Those values are part of criteria used for olfactory ability of animals. Olfactory receptors are typically placed on surface of sensory neuron placed in olfactory epithelium, where they bind various odorants and triggers signal cascade which leads to neuron's membrane depolarization. Therefore, about one half of this work summarizes knowledge of olfactory receptor's molecular biology like their structure, main parts of signal cascade (Gαolf, ACIII, CNG channel, Ca2+ dependent Cl- channel) just as parts needed for steady-state establishment. Expression of olfactory receptors detected in amount of non-olfactory tissues (mussels, sperm, brain etc.), indicate possibly important biomedical roles of this receptors.
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Effects of olfaction and visual stimuli on mate choice decisions in Grey Partridges
Kotasová, Kateřina ; Albrecht, Tomáš (advisor) ; Sedláček, Ondřej (referee)
This theses focuses on olfactory and visual components of mate choice in grey partridge. The aim is to evaluate the importance of melanin-based ornametnation and olfaction in mate choice and to explain the role of sexual selection in the evolution of secondary ornamentation in this socially monogamous galliform bird. To do that several experiments were conducted to (1) assess the ability of individuals to recognize conspecific odour and (2) to estimate the significance of melanin based feather ornamentation in male-male and male-female interactions. In some experiments I manipulated the expression (size) of feather maleanin- based ornamentation while in others I used birds forced to pair randomly to find out how the size of ornament and similarity in ornament expression between males and females (assortative pairing) affects reproductive success and investments (egg hatchability and number of eggs laid).
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The effect of caloric intake and aromatic substances on the human body odour quality
Fialová, Jitka ; Havlíček, Jan (advisor) ; Martinec Nováková, Lenka (referee)
Previous work has shown that mate choice is in part affected by cues associated with quality and condition of potential mate. These cues could be based on visual characteristics or as shown recently on chemical cues. There is growing evidence which suggest that body odour plays relatively significant role in mate choice. Moreover, body odour is to some extent affected by ingested diet. Therefore, we focused on the effect of caloric restriction and garlic consumption on axilary odour. Our results suggest that restoration of food intake improve body odour in terms of pleasantness and attractiveness (probably as a consequence of recommended specific diet), but during caloric restriction it is rated lower. The garlic consumption improves hedonic assessment of body odour. This effect could be ascribed to his well-known health effects. Our findings thus confirm assumption that ingested diet affects body odour and these changes in odour attractiveness could serve as a cue to individual condition and quality.
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Methodology development of the olfactory perception of children with visual and multiple disabilities
Pařízková, Linda ; Šumníková, Pavlína (advisor) ; Květoňová, Lea (referee)
This thesis deals with importance of olfactory perception and possibilities of its development between the children with visual and multiple disabilities. It provides basic information about anatomy and physiology of olfaction, about processing of olfactory stimuli and about the importance of smell for children with visual and multiple disabilities. The main attention is focused on possibilities of olfactory perception development in the Czech Republic, which is presented by a comprehensive summary of the products available on the Czech market. It also deals with the development of the olfactory perception at the School of Jaroslav Ježek.
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Heart Rate Response in Newborns to Relatively Strong and Mild Trigeminal Odorants
Boušová, Jiřina ; Martinec Nováková, Lenka (advisor) ; Brothánková, Pavlína (referee)
The widely accepted view nowadays is that experiencing odours as rather pleasant or unpleasant is, to a certain degree, shaped on a daily basis through individual experience within one's culture via evaluative conditioning or, rather marginally so, via mere exposure to that certain odour. In other words, humans are not born with any fixed set of olfactory likes or dislikes but rather, they acquire them throughout their lifetime. However, olfactory sensation is not a "pure" percept, as odorant stimuli generally elicit a qualitative percept of an odorant - generated mainly by the olfactory nerve - as well as some degree of chemesthesis - a tactile confound of the odour generated mainly by the trigeminal nerve. The olfactory and trigeminal system exhibit complex interactions at both the peripheral and central level of chemosensory processing, which is also reflected in perceptual characteristics of the final percept, including perceived pleasantness (hedonics). If the olfactory contribution alone does not easily predict neonatal odour hedonics, due to newborns' limited previous exposure to chemosensory inputs, one may hypothesize that together with the strength of the trigeminal contribution they may form a significant factor affecting neonatal appetitive/aversive responses to odours. In the present...
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Ability of specially trained dogs to detect human scent which was exposed to different weather conditions over a long period
Machová, Lucie ; Vyplelová, Petra (advisor) ; Jaroslav, Jaroslav (referee)
Survivability of human scent was observed by many scientists mainly in artificial conditions (Hudson et al., 2009; Santariová et al. 2012; Preti et al., 2006). Against this exists a few researches about survivability of human scent in nature conditions (King et al., 1964; Santariová et al., 2016).
On glass tubes were taken individual humant scent from 6 volunteers. Target scents were left outdoor during 2 different periods of year. Winter samples from 3 target people were outdoor from November to May and summer samples from other 3 people from May to December. Always after 3, 4, 5 a 6 months was taken scent from 1 glass tube of each target person. After last time period was taken scent from target people on cotton textile. Scent of other volunteer was taken at the same time for purpose scent deceptions.
Then was observed if dogs will be able to identify these samples corectly. Specially trained dogs on scent identification line-ups get sample from outdoor environment and they should choose the identical human scent from line of 6 samples (with 1 target and 5 deceptions).
The experiment showed, that dogs are able to identify winter samples in all time periods but are not able to identify summer samples in whatever time period. Time periods do not show any diference.
The biggest influence on quality change of individual human scent has intensity of sun radiation.
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Canine Sensory Perception
Němečková, Markéta ; Sedmíková, Markéta (advisor) ; Chmelíková, Eva (referee)
My Bachelor Thesis Summarizes The Findings of The Current Scientific Literature about The Following: The Sensory Perception of Dogs, The Anatomical Structures that Provide Sensorry Perception as Well as The Mechanisms for Transferring Stimuli from The Outside Environment via Receptors and Sensory Organs to The Central Nervous System.
It Analyses Dog's Senses, Specifically Sight, Taste, Smell, Hearing and Balance, Eyesight and Part also Deals with The Issue of Pain. It Presents The Individual Differences Senses the Dog from Other Mammals, Including Mankind and Shortly Using Dog's senses for People.
The Conclusion is Devoted Primarily to The Ranking of Each Dog According to Relevance for Dog's Orientation in Everyday Life and also for The Use of Sensory Dog with Man.
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Testing of sensory abilities of assessors: studying of smell and vision sensitivity
Hýsková, Eva ; Janoušková, Eva (referee) ; Vítová, Eva (advisor)
This diploma thesis was focused on visual and olfactory senses and their role in the sensory evaluation. The requirements for assessors working in sensory analysis, for testing place and requirements of the Czech Institute for Accreditation for the accreditation of laboratories working in the field of sensory testing are summarized here. An overview of methods and legislation in the field of sensory analysis is also presented. Methods for studying of smell and vision sensitivity of assessors were selected in the experimental part of this thesis. The methods were verified in the sensory laboratory, validated and standard operating procedures have been developed to the individual methods.
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