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Probiotics, their influence on health and probiotic food
Hondlíková, Tereza ; Dostálová, Jana (advisor) ; Plocková, Milada (referee)
Probiotics are living microorganisms which show positive effects on human health if consumed in a sufficient quantity. Probiotics have been under a lot of research recently, however their effectiveness in terms of treatment or prevention of diseases has not been supported by a sufficient number of studies and research yet. The thesis summarizes the existing knowledge about probiotics, characterizes individual types of probiotic organisms, describes possible sources of probiotics and their impact on human health. Theoretical part of the thesis deals with the definition of the concept of probiotics, also describes prebiotics and synbiotics which are closely related to this topic. characteristics of individual groups of probiotics as well as their impact on human health and possible use in the treatment of diseases as a form of biological treatment are also included. Furthermore the sources of probiotics both in the form of foods containing probiotics and in the form of probiotic dietary supplements are described. At the end of the theoretical part, recommendations for receiving and selecting a suitable source of probiotics are summarized. The aim of the practical part was to find out if the general public is aware of probiotics. Questions about preferences in the selection and consumption of...
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Biofilm formation in probiotic cultures and its application in pharmacy
Ryšávka, Petr ; Obruča, Stanislav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
The work was comprehensively focused on the development of adhesive forms of probiotics in the form of a biofilm on combined carriers with a prebiotic component. The second part dealed with the influence of food on the multiplication and survival of selected types of probiotic bacteria. Subsequently, the effect of individualized probiotic supplements on changes in the human intestinal microbiome was monitored. Suitable adherent probiotic strains for biofilm formation were selected and tested. Methods have been introduced and different variants of carriers for culturing and binding bacteria have been tested. In vitro experiments verified the stability of biofilm stucture and its resistance to low pH, bile and antibiotics in comparison with the planktonic cell form. The antimicrobial effect of probiotic strains in the form of a biofilm was studied. The cultivation of the multispecies biofilm on the combined carrier was optimized and the stability of the biofilm and the final viability of probiotic bacteria were confirmed. Furthermore, the influence of various foods and beverages on the viability of probiotic bacteria was evaluated with emphasis on the simulation of passage through the gastrointestinal tract. Both models, solutions with standardised concentrations of alcohol, sugar, salts, proteins or different pH and different types of real foods and beverages were tested. The effect of food and beverages was tested on monocultures of Lactobacillus acidophilus, Bifidobacterium breve and on probiotic capsules containing a mixed culture of probiotic microorganisms. The survival of probiotics in various food matrices in the simulated gastrointestinal tract was quantitatively different. We managed to define foods suitable for supporting the multiplication of probiotic bacteria. A separate part of the work was focused on the targeted modulation of the intestinal microbiome by individualized probiotics that were prepared on the basis of molecular biological analyzes of the intestinal microbiome aimed at detecting the percentage of lactobacilli, bifidobacteria and phylum Firmicutes and Bacteroidetes. Personalized probiotic supplementation confirmed the positive effect of this approach on microbiome changes, especially on the increase of the content of lactobacilli, bifidobacteria and the overall diversity of the microbiome.
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Changes in microbial colonization of gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation
Michková, Petra ; Mrázek, Jakub (advisor) ; Kodešová, Tereza (referee)
Introduction: Physiological microflora is characterised by wide diversity. The microbial community is mostly composed of bacteria, but also includes fungi, archaea and viruses. Anaerobic commensal bacteria (Firmicutes and Bacteroidetes) dominate 90% of the colon. The composition and products of the gut microbiota have a significant effect on an individual's immune system, and their interactions may ultimately promote immune tolerance or inflammatory immune response. Blood cell transplantation (HSCT) and its associated standard procedures of conditioning, antibiotic exposure and dietary prophylaxis represent modification and disruption of the gut microbiota leading to the development of some serious post-transplant complications affecting the OS (overall survival) and TRM (treatment related mortality) of patients. Objectives: The aim of this work was to investigate the representation of individual bacterial strains in patients undergoing allogeneic HSCT, as well as the effect of transplantation on the composition and diversity of their gut microbiota. Methods: Stool samples were obtained from 52 patients who underwent an allogeneic hematopoietic cell transplant at the Institute of Hematology and Blood Transfusion in Prague. A cut-off date for the first sample was set for the start of...
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Probiotics, prebiotics, synbiotics - are they really effective?
Šmídová, Zuzana ; Pejšová, Hana (advisor) ; Pechar, Radko (referee)
Probiotics and prebiotics constitute an important part of our nutrition and possess many positive effects on human organism. Synbiotics represent combination of both of them. The aim of the theses in the theoretical aspect was to create a systematic review of available knowledge on the given topics and in the practical part to assess the level of awareness on these topics with regard to their presence and effects. In the practical part of the theses the questionnaire survey methodology was used. After evaluation of all answers it was found out that most people are familiar with the term probiotics, less people with the term prebiotics and the least respondents with the term synbiotics. Fermented dairy products are consumed by 88,3 % of respondents, mostly 2-3 times a week. The rest not consuming them indicated mostly the reasons of bad flavour or some form of intolerance. The respondents know also other fermented foods (sauerkraut, vegetables), however, these are less consumed than fermented dairy products. The least often the food supplements containing these substances are consumed. Due to the low level of awareness on prebiotics and synbiotics it would be very appropriate to improve public awareness on the given topics in order to increase their consumption, mainly in the form of their natural...
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Dietary fibre as a disease prevention: the intake of adolescent and adult
HORBANIUCOVÁ, Tereza
The main theme of the bachelor thesis is Dietary fibre as a diseases prevention: the intake of adolescents and adults. Theoretical part focuses on the information about dietary fibre, its sources and its distinction on water-insoluble dietary fibres and water-soluble dietary fibres and its subtypes. Furthermore, the thesis gives information about dietary fibre's effects on diseases prevention. Lastly, recommended intake of dietary fibre for adults is given as well as adverse effects of dietary fibre when it is taken in higher quantity. Goal of the practical part was to learn about people's general knowledge of dietary fibre and its effects on human organism and to learn about their diet in terms of dietary fibre intake. The goal was fulfilled by questionnaire. Another research goal was to compare dietary fibre intake of adolescents and adults, recommended intake of dietary fibre was considered. This goal was maintained through diet notes which respondents made for four days. These diet notes where calculated in "Nutriservis Professional". Research group had 10 respondents which were divided into two groups. First group consisted of adolescents in the age of 14-16. Second group was formed by adults in the age of 20-25. Based on the results of our research most of the respondents have knowledge about dietary fibre and its effects on human health. From the second part of our research we can assume that most respondents' intake of dietary fibre is insufficient and it does not even cover half of recommended dietary fibre intake.
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Fiber intake in developed countries and possibilities for its increase in population
Ježková, Kristýna ; Pejšová, Hana (advisor) ; Křížová, Jarmila (referee)
This diploma thesis deals with fiber intake and possibilities of its increase. The main aim of the thesis is to evaluate the actual intake of fiber in the population and to find out whether people actually know what fiber is and what should be its daily intake. The theoretical part is focused on fiber itself. It describes its characteristics, distribution, properties and sources. The effect of fiber on human health and the consequences of insufficient or excessive fiber intake are discussed as well. The practical part is focused on the real intake of fiber in the population. It is done in the form of a questionnaire survey, in which I try to find out what knowledge of fiber people have, but also how often they consume foods containing fiber. Most of the questionnaires were handed out to customers of one unnamed pharmacy and healthy nutrition center in Prague and other passers-by. Altogether, I managed to collect 84 questionnaires. The questionnaire results show that vast majority of respondents have an idea of what fiber is and which foods contain it most; however, they do not know what the recommended daily intake is. Still, half of the respondents think their diet includes enough fiber. The fiber intake in men and women was not much different. The most significant difference was in the...
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Encapsulation of probiotics and prebiotics for use in nutritional products for children
Šnajdarová, Karolína ; Němcová, Andrea (referee) ; Skoumalová, Petra (advisor)
The Diploma thesis deals with designing of probiotic dietary supplement for children with strains Lactobacillus acidophilus and Bifidobacterium breve and with prebiotics. Used prebiotics were Inulin, Chia fiber, Bamboo fiber, Chlorella + Spirulina and Yakon syrup. The theoretical part is focused on probiotics, prebiotics and their biological influence. In experimental part the possibilities of encapsulation into alginate particle and lyophilisation of probiotic cells were observed to find their good form to final nutritional product for children. Several types of probiotic with addition of prebiotics were tested in model conditions of gastrointestinal tract. It was found that addition of prebiotic highly increases viability of probiotic cells and their resistance to model conditions of gastrointestinal tract. In this case, the best prebiotic was found in Yakon syrup. The prebiotics were also characterised in terms of nutritional composition, amount of total and reducing sugars, oligosaccharides, proteins, lipids, polyphenols and chlorophyll were obtained. Finally, Chia fiber, Chlorella + Spirulina and Yakon syrup were chosen as prebiotics with best characterisation/properties. In conclusion, a dietary supplement with lyophilized alginate particles containing probiotic cells and with the most appropriate prebiotics were designed. Forms of the product were powder and gummy-bear.
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Consumption of fiber in old age
ŠÁLEK, Jan
The theme of this bachelor thesis is the Consumption of fiber in the old age (seniors). Formerly, the dietary fiber was considered unnecessarily burdensome for human organism. Later, it was found that it has a relatively large impact on human health, especially by serving as a prebiotic for symbiotic bacteria inhabiting the gastrointestinal tract. There is still prevailing opinion that roughage divides into a soluble and insoluble, despite that some types of fiber cannot be definitely classified and placed into any of these groups because they have characteristics of both of them. The daily recommended dose is 30 g. The qualitative research was used for elaboration of the practical part. The first goal was to find out how much fiber seniors consume. The second aim was to compare the consumption of fiber among elderly people living alone and the elderly living in retirement homes built for them. The research group, at the first research question, consists of 20 seniors from the Southern Region aged over 65 years. The research group, at the second research question, consists of five retirement homes from the whole Czech Republic. All respondents were given a record sheet for one week, and instructions on how to fill it. Acquired menus were subsequently re-calculated through the program "Nutriservis Professional". The results show higher consumption of fiber than the average in the Czech Republic, but there is still almost a half missing to get the recommended daily dose. There was observed BMI and its impact on the consumption of fiber. No significant effect was found, because overweight had all interviewees except one respondent. The financial limit is visible for evaluated diet of homes for the elderly, therefore, there are no whole grain products, vegetables and almost no fruit. Despite these limitations, the average consumption of fiber is almost equal to elderly people living alone. In conclusion, there is a proposition how to, despite the low funds, increase the intake of roughage, and avoid or, at least, reduce the problems associated with its low consumption. Recommendations for respondents are individual and given under each evaluation.
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Fiber and its consumption on a hospital bed
KŘÍŽKOVÁ, Ivana
The theme of the bachelor thesis is Fiber and its consumption on a hospital bed. Dietary fiber is defined as a carbohydrate food ingredient that cannot be transformed by one´s own enzymes. Fiber was originally considered a ballast food component. Gradually, it was found that it is a part of food fermented by colonic bacteria. Fiber is substrate for these bacteria and they transform it into substances intended for human organism. Fiber can be divided into soluble and insoluble. Both types are essential for human nutrition. A recommended fiber intake for an adult is 30 g per day. In the practical part of the bachelor thesis, was used qualitative research method. The research sample consisted of hospital two-weeks menus. One diet was applied in the hospital facility in the Central Bohemian region and one in the South Bohemian region. Using a computer program "Nutriservis Professional" I calculated the fiber content in the individual meals. The findings were compared with the recommended daily intake published in the book called Referenční hodnoty pro příjem živin and S výživou po celý rok. I also calculated energy value, amount of proteins, fats and carbohydrates of the menus. The results were compared with the recommended values. As a research I interviewed the nutritional therapists in the hospital facilities about the patient´s diet and possible solutions to health problems caused by a lack of fiber. The interviews consisted of eight open questions focused on the fiber consumption, denying of foods containing fiber. The results of calculation previewed the menu at the first hospital facility that fiber deficiency occurs primarily in a liquid diet, while for rational diet, diabetic diet and for infants and older children the fiber content is sufficient. The menu of the other hospital contains insufficient amount of fiber, especially for diabetic diet or geriatic diet reducing fat. On the other hand, diabetic diet contains a plenty of fiber. The interviews that were supposed to find out how nutritional therapists approch the fiber issues in diets brought similar responses. The hospital in the Central Bohemian region include into the diet especially fruit, vegetable, whole grain products and legumes. Older patients ages constipation suffering from bowel obstuction, intestinal disease and flatulence frequently refuse fiber, especially legumes. If a patient has health problems caused be the lack of fiber nutritional therapists usually solve this problem by education and inclusion of fruit, vegetable and whole meal bread into the patient's diet. The hospital in the South Bohemian region frequently include into the diet potatoes, rice, fruit, legumes, vegetable and whole grain breads. Fiber is often rejected by older people and people suffering from diarrheal diseases. Health problems resulting from the lack of fiber are often solved by recommending by foods containing fiber or the nutritional supplements such as NutriFibre.
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Probiotics and prebiotics and their co-encapsulation
Šnajdarová, Karolína ; Bokrová, Jitka (referee) ; Márová, Ivana (advisor)
This bachelor thesis is focused on study of probiotic cultures and options of coencapsulation with specific substrates called prebiotics. Lactobacillus acidophilus and Bifidobacterium breve were used as encapsulated microorganisms. As the specific substrates inulin, psyllium, apple fiber and hemp fiber were tested. Prebiotics was added to probiotics both in untreated form and in hydrolyzed form. The stability and viability of the cells have been studied by microscopy for the period of 6 weeks. Particles were stored in the enviroment of 2.5% citric acid. As the best substrate for cell growth the hemp fiber was found. The hemp fiber exhibited the highest increase of cells count. During observation of long-term stability in the model digestive conditions, particles with hemp fiber were destroyed in instestinal juices. For that reason, particles with hemp fiber could be used in food supplements with targeted transport. The bachalor thesis is also focused on characterisation of prebiotics components. The highest amount of total sugars in the group of non-hydrolyzed prebiotics exhibited psyllium. The highest content of reducing sugars both in the groups of non-hydrolyzed and hydrolyzed prebiotic had hemp fiber. By the method of thin-layer chromatography was found out that the main components of hydrolyzed prebiotics are glucose and fructose. Further, some disaccharides and oligosaccharides were obtained by TLC analysis. For characterisation of prebiotics by HPLC the column Rezex exhibited better sensitivity. HPLC analysis confirmed that the main components of prebiotics are glucose and fructose, some more complex carbohydrates were found too.
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