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Determination of the stoichiometry of the copper complexes with dehydrosilybine A
Klimková, Kateřina ; Mladěnka, Přemysl (advisor) ; Hrdina, Radomír (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Kateřina Klimková Supervisor: Assoc. Prof. Přemysl Mladěnka, Pharm. D., Ph.D. Consultant: Assoc. Prof. Kateřina Valentová, Ph.D. Title of Thesis: Determination of the stoichiometry of the copper complexes with dehydrosilybin A Silymarin, the standardized extract of the milk thistle (Silybum marianum), is a widely used approved over-the-counter drug that is recommended for a number of liver diseases. Silymarin contains as one of its components 2,3-dehydrosilybinA, which has an appropriate metal binding site in its structure. In general, flavonolignans, due to their structure, can interact with transition metals in the gastrointestinal tract by forming complexes. This property can be useful for the protection against excessive amounts of metals in the body. The aim of this in vitro study was to analyse the interaction of 2,3-dehydrosilybin A with copper, which plays a crucial role in the organism as a cofactor of many enzymes. Although being an essential element, it can, however, be toxic at elevated levels. Stoichiometry, as one of the most important characteristics of the complex, was determined by UV-Vis spectrophotometry in four (patho)physiological pH conditions (4.5; 5.5; 6.8; 7.5)...
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Assessment of the complex stoichiometry of the active dexrazoxane metabolite ADR-925 with iron and copper by the standard Job's method
Szotáková, Tereza ; Mladěnka, Přemysl (advisor) ; Jirkovský, Eduard (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Tereza Morávková Supervisor: Assoc. Prof. Přemysl Mladěnka, Pharm.D., Ph.D. Title of Thesis: Assessment of the complex stoichiometry of the active dexrazoxane metabolite ADR-925 with iron and copper by the standard Job'smethod Iron and copper are important trace elements which participate on many physiological processes in humans. Their kinetics in the organism is tightly regulated since both lack or excess of these elements are associated with pathological states. Free ions of iron and copper can catalyse reactive oxygen species (ROS) production and hence cause damage to proteins and DNA. Imbalance in these metals is linked with diabetes, cardiotoxicity, cirrhosis of the liver and neurodegenerative diseases as Alzheimer's disease or Parkinson's disease. Dexrazoxane is a drug with documented protecting effect against cardiotoxicity of anthracyclines. A former theory associated its protective effects against these cytotoxic drugs with the iron-chelating properties of its active metabolite ADR-925. The goal of this diploma thesis was to explore if ADR-925 is able to chelate Fe2+ , Fe3+ , Cu+ a Cu2+ ions at physiologically and pathophysiologically relevant pH values (4,5; 5,5; 6,8 and 7,5) and...
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Food quality impact on growth and survival of Daphnia longispina (Crustacea: Cladocera)
Sýkorová, Veronika ; Sacherová, Veronika (advisor) ; Macháček, Jiří (referee)
This thesis focuses on ecological processes among plankton organisms. It especially questions the influence of food quality on growth and survival of freshwater plankton. I focused on the influence of various ratios of nitrogen and phosphorus in available food. I am also interested in finding out the food requirements of chosen consumer Daphnia longispina. Within this broad topic, I specify a question, whether various quality and quantity of food influences the life history parameters of the species Daphnia longispina and whether the absence of common species of Cladoceran Daphnia longispina in Plešné Lake is caused by unsuitable quality of food. I have bred Daphnia longispina in laboratory conditions for two years. I fed it by algae Monoraphidium dybowskii isolated from Plešné Lake and grown in two media P-limited (C:P 1174), P-unlimited (C:P 290) and three concentration (1,2, 4 mg C/L). Cladocerans fed by food from medium that had a C:N:P ratio approximately the same as can be found in Plešné Lake (C:P 1174) lived for a singificantly shorter period of time and during the experiment they did not reproduce even in the case with the largest amount of food (4 mg C/L). This is a possible reason why Daphnia longispina did not return to Plešné Lake after the period of strong acidification, climax of...
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Diet quality impact on growth and survival of freshwater zooplankton species
Sýkorová, Veronika ; Sacherová, Veronika (advisor) ; Nedbalová, Linda (referee)
Food quality has a significant influence on freshwater zooplankon. It influences its growth rate and development generally. By quality we understand a certain stoichiometric ratio of the main biogenic elements (carbon, nitrogen, phosphorus) in the biomass of food. Ecological stechiometry is helping to understand the balance of chemical elements in ecological interactions and processes. Freshwater zooplankton consists mostly of two groups of organisms: rotifers (Rotifera) and crustaceans (Crustacea) - cladocerans (Cladocera) and copepods (Copepoda). Each of this groups requires different quality of food. Most of filtering cladocerans are fast- growing organisms, which need a phosphorus-rich food for their fast development (small C:P ratio in food) and vice versa for most of the copepods. The food offered is not always ideal for all members of zooplankton, that is why various pre- and post- absorptional mechanisms developed to help them to cope with unsuitable food. The ability to survive unvafourable conditions is closely linked to the zoolpankton's reactions to unsuitable C:N:P ratio in its food. The C:N:P ratio in food of zooplankton also influences the species distribution in water bodies. Key words: stoichiometry, food quality, zooplankton
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Re-design of biomass boiler for very wet fuel
Zbořil, Martin ; Skála, Zdeněk (referee) ; Lisý, Martin (advisor)
The aim of this thesis is to calculate the stoichiometric balance of boilers and boiler fuel for normal monture to 60%. Determine the monture, content depending on the conditions of the fuel in the combustion chamber and conditions of combustion. Experimental analysis of the current state of the boiler ZKG measurements using this device and propose modifications and solutions.
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Desulphurization of pulverized coal bouler (dry bottom ash) in Teplarna Olomouc
Haluza, Jakub ; Lisý, Martin (referee) ; Skála, Zdeněk (advisor)
This master thesis deals with the feasibility studies desulphurization of powder granulation K3 boiler at Olomouc heating station. K3 boiler burns black energetic coal and after January 1st, 2016 will not meet new more strict emission limits. The theoretical part of the thesis charts usable desulphurization methods from which the semi-dry NID appears as the most appropriate one. In the practical part there is performed a stoichiometric calculation of the flue gas and the balance of raw materials and output products. The possibility of NID method usage is confirmed here. Next the annual operating cost of the desulfurization process is calculated and some NID systems already installed references are presented.
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Fireplace Inserts
Ptáček, Martin ; Štelcl, Otakar (referee) ; Fiedler, Jan (advisor)
The goal of this thesis is examination of automatic controller in various operating modes on fireplace inlay Venus 12.1.W. The work compares few measurement, always with measurment without automatic controller In this measurements are evaluated the emissions of CO, CO2, oxygen content in the flue duct gasses and the temperature in the flue duct are listed here and the efficiency is calculated for different operating modes. Conclusion contains overall summary of this thesis. At the end there is a search of manufacturers not only in Czech Republic
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Fuid bed boiler for biomass
Bytešník, Jan ; Skála, Zdeněk (advisor)
This master thesis designes the calculation of fluid boiler with specific part - the circular fluid bed. The heat is given by combustion of wood biomass. The thesis is devided into several parts. All necessary elementary results are going to be reached within these parts: an analysis of solid fuel, stechiometry calculation, discussion on output limits and the environmental point of view, definition of elemental heat losses and general heat efficiency, calculation and design of a combustion part and its heat loads and calculation of enthalpies with different ash concentrations. All these phases get to a successful design of the fluid boiler heat balance and sizes of heat-flow surfaces. The composition of the boiler shows the added drawing.
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Burners for liquid and gaseous fuels
Zaidullina, Liliia ; Baláš, Marek (referee) ; Kracík, Petr (advisor)
This Bachelor’s thesis is focused on the burners for liquid and gaseous fuels, primarily on the ones that are used in industrial technological equipment. This is a detailed research of available sources including publications and websites in order to describe the classification and construction of burners and their operation and also standards that must be observed. Three main objectives were defined for the paper. The first one is to describe the different types of burners, the basic construction concept of the burners on liquid and gaseous fuels. Usually, the construction of the burner depends on technological conditions of their application. The second objective is to describe the legal standards and basic parameters of the burners. These standards are applicable to industrial burners for gaseous and liquid fuels, which burn with mixture air and fuel. The standards cannot be applied to the burners, in which the additives such as electric or acoustic energy and oxygen. The third objective is to describe the method of offering and calculating burners.
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