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Molecular detection of genes involved in drought stress reaction in opium poppy
ŠEFČÍKOVÁ, Miroslava
Drought belongs among one of the main abiotic factors that influence the yield and quality of agricultural crops. However, plants fight these negative environmental impacts by mechanisms developed during evolution. One of these mechanisms is the plants´ response on molecular level, which is gene expression. These genes potentially reacting to drought include the following genes: MLP43 and SDD1. For the purposes of molecular detection of these genes, the DNA from opium poppy plants (Papaver somniferum L.) Orel and Onyx type, was necessary to be isolated. Further, primer sets for the genes of MLP43 and SDD1 were designed. The chosen primer sets were used for analysis purposes using the real-time PCR method. The PCR method was also optimized.
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Impact of nitrogen fertilization and drought on gliadins content in winter wheat
Odstrčilová, Eva ; Obruča, Stanislav (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.
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Effect of drought on the metabolism of tobacco plants
Miedzińska, Lucia ; Ryšlavá, Helena (advisor) ; Vaňková, Radomíra (referee)
EEffffeecctt ooff ddrroouugghhtt oonn tthhee mmeettaabboolliissmm ooff ttaabbaaccccoo ppllaannttss (Nicotiana tabacum L.) Diploma thesis - abstract Lucia Miedzińska, Bc. Work Supervisor: Doc. RNDr. Helena Ryšlavá, CSc. Consultant: RNDr. Veronika Doubnerová, PhD. Drought stress is one of the most frequent form of plant stress, which occurs not only under condition of limited water availability, but also under reduced ability to accept water by roots, for example in salted or cold soils. In this thesis the changes in enzyme activities of: NADP-malic enzyme (EC 1.1.1.40; NADP-ME), phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC) and pyruvate, phosphate dikinase (EC 2.7.9.1; PPDK) in tobacco plants (Nicotiana tabacum L., cv. W38) after drought were investigated. Enzyme activities in tobacco leaves were significantly increased during 11 days of stress, PEPC 2-fold, PPDK 3,3- fold and NADP-ME 4-fold compared to control plants. The regulation of NADP-ME and PEPC activities were studied on transcriptional level - by the real-time PCR method and on translational level - immunochemically. The expression of NADP-ME protein and transcription of mRNA for chloroplast NADP-ME isoform were increased, but mRNA for cytosolic isoform was not affected. The protein expression of PEPC was slightly increased, transcription of...
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Environmental factors effect to the Scotch pine seedlings survival
Pourová, Marie ; Ulbrichová, Iva (advisor) ; Janeček, Vladimír (referee)
The survival and mortality of the Scots Pine (Pinus sylvestris) seedlings during the vegetation season of 2015 are evaluated in the thesis. The seedlings in greenhouse conditions in Truby were exposed to water deficiency, three variants of shading and simultaneously to environmental temperature, which, due to weather, was extraordinary high at the time of the experiment.
The highest number of grown seedlings from the seed material from Plasy was 89 % from the total of 224 outplanted ones, namely in conditions of mean shading and slightly reduced watering. The highest mortality of the seedlings was found in unshaded environment with slightly reduced watering, in mean shading with the second lowest watering and in the highest shading with the second lowest watering, the mortality was 100 % there. The seedlings from the seed material from Doksy were much more hard put to it. The seedlings grew only in four cases in unshaded environment with the highest watering (4 % from 224 outplanted ones), in mean shading with the highest watering (38 %) and in the environment with the highest shading together with the highest watering (68 %) and slightly lowered watering (1,3 %). In all cases the seedlings mortality was higher than 70 %.
The lowest number of grown seedlings occurred in unshaded environment and between the variants with mean and high shading, there was only a small difference. The results were strongly affected by extraordinary warm vegetation season.
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Effect of drought stress factor on glycoalkaloid contents in potato (Solanum tuberosum L.)
Matoušková, Vendula ; Kotíková, Zora (advisor) ; Martinková, Jaroslava (referee)
Potatoes are an important and irreplaceable crop. This kind of crop is very important not only for it is use but also for a nutrition composition. There are also a prominent source of vitamins, minerals and antioxidants. Outside substances beneficial to health and potatoes contain harmful substances. These substances are foreign or naturally occurring, which include toxic glycoalkaloids. Glycoalkaloids are secondary metabolites of plants. Glycoalkaloids in potatoes have protective function it can increase the synthesis for example in case of pest infestations, mechanical damage or in case of to much light and heat. The potatoes were found several glycoalkaloides. Main, which constitutes 95 % of their content, are alpha-chaconine and alpha-solanine. Their toxicity is inhibition of acetylcholinesterase and breaking the cell membranes. The potato tuber is their content is distributed unevenly. The quantity of glycoalkaloids is affected by manny factors as for example place, year, kind, the way how the crops are grown and storage. In Czech Republic the maximum allowed limit of glycoalkaloides in potatoes were made by legislation on 200 mg/kg fresh potato matter. In the commonly grown varieties of the amount is far below the hygienic limit. The methods for isolation of glycoalkaloids in potatoes are mainly chromatographic. The most commonly used HPLC (high performance liquid chromatography). In performed experiment was determined the content of majority glycoalkaloid alpha- chaconinu and alpha-solaninu at four different kinds -Milva, Marabel, Laura and Valfi. Drought stress has been studied for their content, assuming their accumulation in comparison with the other two variants - irrigation watering and drip irrigation. The glycoalkaloides content were messured by the UHPLC/MS/MS. The obtained results concluded that the content of glycoalkaloids is the variety dependent. Drought stress can probably increase their content. In our experiment, it positively did not. Important is the choice of kind in case if expectation a hot and dry year of growing. Kinds Milva and Marabel are very good in these conditions. In the case of general principles for cultivation, storage and cooking, the glykoalkaloids does not vision a risk for the consumer.
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Vliv zvýšené koncentrace CO2 a sucha na rychle rostoucí dřeviny
Vágner, Lukáš
The main objective of this study was to determine the effect of elevated CO2 and drought on growth of fast-growing trees, hence hybrids of poplar Populus nigra and Populus maximowiczii on experimental station Domanínek. These parameters were measured: photosynthesis, reflectance, fluorescence of chlorophyll and content of chlorophylls and flavonols. The results show a demonstrable effect of greater regulatory abilities stomatas at elevated CO2 concentration, which leads to greater efficiency of water use. Decisive influence on leaf area, specific leaf area and the dry weight has the effect of drought. However, for specific leaf area, contrary to expectations, there was an noticeable increase during drought period. In the case of other characteristics come to reduce them. By contrast specific leaf area slightly decreases at elevated CO2 concentration. Rate of photosynthesis increases with increasing concentrations of CO2, while the effect of drought dampens these characteristics and also reduces the chlorophyll content. Increasing concentration of CO2 during the experiment also leds to an increase in NDVI index, which shows increased chlorophyll content. The values of fluorescence at two concentrations of CO2 when exposed to drought stress differed minimally. The maximum quantum yield of photosystem II is slightly higher at elevated CO2 concentration. Overall, it can be summarized that in the short-term effect of drought stress in hybrid poplar, increased concentration of CO2 increases water use efficiency and thus to mitigate the impact of drought on photosynthetic parameters.
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The response of Norway spruce and European beech's photosynthetic apparatus to some stress factors - comparative study
NOVÁKOVÁ, Hana
In the context of expected climate changes is more and more important to study how will species of present ecosystems react to this changes. Thus this study is focused on reactions of two main forest tree species of Central Europe ? Norway spruce (Piacea abies L.) and European beech (Fagus sylvatica L.) ? to water stress, higher quantity of N in soil, combination of theese stress factors and heat stress. The effects of stress were monitored on maximal photochemical efficiency of PS II (Fv / Fm) and on contents of photosynthetic pigments. It was found that Norway spruce is more resistant to all mentioned stress factors than European beech.
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