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Artificial phospholipid membranes as models of real membranes and transport of charged particles across them
Parisová, Martina ; Navrátil, Tomáš ; Šestáková, Ivana ; Kohlíková, E. ; Petr, M.
Because the real bio-membranes and the tranporting processes realized across them are very complicated, the model membranes have been used for elucidation of the basic transporting steps. In the contribution, the different possibilities of characterization, description, and elucidation of the transport processes on the example of some metal ions and theor complexes, i.e., of cadmium, lead, and copper, were presented. It summarizes the results achieved using supported phospholipid model membranes formed on porous membranes and on agar support. Discussed transporting processes of selected ions have been characterized using two electrochemical methods: electrochemical impendance spectrometry (EIS) and voltammetry. The impact of some parameters on these systems and processes was investigated.
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Electroanalysis with phthalocyanine modified HOPG electrodes
Mansfeldová, Věra ; Janda, Pavel
In this papers we presented modification of highly oriented pyrolitic graphite (HOPG) by two different phthalocyanine (Pc) derivatives and two different deposition techniques. We have investigated electrode modified by fullerene-derived phthalocyanine and cobalt tetraneopentoxyphthalocyanine (CoTNPc) to consider how different modification procedure-electrodeposition and drop evaporation and composition of phthalocyanine influences detection of model compound, L-cysteine.
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Electrochemical and spectrometric study of FOX-7 in aprotic solvents
Šimková, Ludmila ; Ludvík, Jiří ; Klíma, Jiří
2,2-Dinitroethene-1,1-diamine (FOX-7) is a recently developed and broadly tested energetic material with high efficiency and low sensitivity. Generally, explosion is based on thermally initiated chain of intramolecular redox reactions. Recently it has been found that electrochemical reduction in aqueous solutions is also able to provoke the chain of follow-up processes leading to total decomposition of the parent substance yielding gaseous products analogously like during explosion. Similarly, results from reductive electrolysis of FOX-7 in aprotic solvents show analogous course of degradation. The first transferred electron activates the molecule and initiated the formation of colored radical intermediates that have been spectroscopically characterized
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Voltammetric determination of anticancer drug flutamide at screen-printed carbon electrodes
Vyskočil, V. ; Navrátil, Tomáš ; Barek, J.
Flutamide, 4-nitro-3-trifluoromethyl-isobutylanilide, is the active component of a synthetic nonsteroidal antiandrogen drug, which is commonly used in the treatment of advanced prostate cancer. In this contribution, optimum conditions have been found vor voltammetric determination of flutamide by DC voltammetry (DCV) and differential pulse voltammetry (DPV) at screen-printed carbon electrodes (SPCEs). Under optimum conditions (Britton-Robinson buffer pH 7.0 - methanol (9:1)), linear calibration curves were measure with limits of quantification 6x10(-7) molL(-1) and 8x10(-7) molL(-1) for DCV at SPCEs, respectively. The newly developed methods were successfully applied to the determination of the drug in pharmaceutical formulation Apo-Flutamide (Eulexin tablets).
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Bioimpedance methods to measure somatometric and volumetric changes
Petr, M. ; Kohlíková, E. ; Navrátil, Tomáš
Assessment of human body composition has stimulated the need for an understanding of available methodologies of estimating fat free mass and percent body fat. In our study, we found statistical significant changes of body composition evaluated by multi-frequency bioelectrical analysis in a group of athletes following creatine supplementation. These results are consistent with literature i.e. supplemented creatine effects osmolality of cell´s membranes which leads to increased intracellular water retention. Multi-frequency BIA can be considered to be an accurate and sensitive method for assessment of human body composition.
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