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Laboratory diagnostics of hepatitis E - experience from routine laboratory
Tužáková, Andrea ; Janďourek, Ondřej (advisor) ; Diepoltová, Adéla (referee)
Charles University Faculty of Pharmacy in Hradec Kralove Department of Biological and Medical Sciences Study program: Specialist on Laboratory Methods Author: Andrea Tužáková Supervisor: PharmDr. Ondřej Janďourek, Ph.D. Title thesis: Laboratory diagnosis of hepatitis E - personal experience in a routine laboratory This thesis takes a comprehensive review of viral hepatitis E (VHE), including theoretical and practical aspects. In the theoretical part, the VHE is presented in detail, including epidemiology, genotypic variants, clinical symptoms, diagnostic methods, prevention and treatment. The epidemiological trends and prevalence of VHE are analysed, along with genotypic variants and their correlation with clinical manifestations. Various diagnostic modalities and therapeutic options in the context of VHE are discussed. Hepatitis E is a liver infection caused by the hepatitis E virus. It is one of the five main types of hepatitis viruses: A, B, C, D, and E. Hepatitis E virus is transmitted mainly through the faecal-oral route, often via contaminated water or food. It can cause acute illness characterized by jaundice (yellowing of the skin and eyes), dark urine, fatigue, abdominal pain, and loss of appetite. Most cases of hepatitis E resolve on their own within a few weeks to months without causing...
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Diagnostics of pathological changes in vascular system and innovative methods of interventional therapy
Novák, Matěj ; Lambert, Lukáš (advisor) ; Křivánek, Jiří (referee) ; Cihlář, Filip (referee)
Purpose. The objective of the first study was to compare the results of primary patency of failing dialysis arterio-venous fistulas and grafts when using drug-coated balloon catheters in comparison with simple balloon catheters. The aim of the second study was to quantify perfusion changes on CT pulmonary angiography (CTPA) in patients with chronic thromboembolic pulmonary hypertension (CTEPH). Methods. In the first study, a total of 76 patients were randomized to the study - 38 patients were treated with percutaneous transluminal angioplasty using a drug-coated balloon catheter, and 38 patients were treated with percutaneous transluminal angioplasty using a simple balloon catheter. Follow-up DSAs were performed at 3, 6, 9, and 12 months after baseline intervention. In the second study, CTPA in 58 patients with CTEPH were evaluated. The volume of the hypoperfused part of the lungs was measured using density thresholding in semi-automatically segmented lung volume and compared with hemodynamic parameters from catheterization. Results. In the first study, the primary patency in the PTA group with a drug-coated balloon catheter versus the PTA group with a simple balloon catheter was 86.7 ± 5.6% vs. 74.2 ± 7.4% at 3 months, 52.8 ± 8.4% vs. 25.6 ± 7.9% at 6 months, 21.8 ± 8.1% vs. 11.0 ± 5.9% at 9...
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Evaluation of breast milk production in relation to body composition in Czech lactating women.
Bugošová, Andrea ; Hronek, Miloslav (advisor) ; Kovařík, Miroslav (referee)
The aim of this study was to evaluate the production of breast milk in relation to the body composition of Czech breastfeeding women and to compare the results to similar studies. Six breastfeeding women participated in this specific part of the longitudinal study, undergoing 4 examinations during a 9-month lactation period. Anthropometric methods were used to measure basic body parameters. Furthermore, body composition was assessed using dual bioimpedance analysis with a digital scale and spectroscopic bioimpedance analysis using the Body Composition Monitor device. According to Friedman's nonparametric paired test, statistically significant differences between the examined periods were found in BMI values (p = 0.029), BSA (p = 0.019), waist circumference (p = 0.048) and hip circumference (p = 0.031). These values fall below the significance level of 0.05. According to Spearman's correlation, breast milk production positively correlated with pre-pregnancy weight (p = 0.003, r = 0.817), pre-pregnancy BMI (p = 0.002, r = 0.847), pregnancy weight (p = 0.044, r = 0.624), bone mass measured by BIA (p = 0.020, r = 0.697), basal metabolism measured by BIA (p = 0.028, r = 0.670), FM mass measured by BIA (p = 0.034, r = 0.651), chest circumference (p = 0.003, r = 0.825), waist circumference (p = 0.025, r =...
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The use of Dynamic Neuromuscular Stabilisation exercise in female basketball players with deep stabilisation spinal system dysfunction
Janderová, Petra ; Plevová, Kristýna (advisor) ; Motáková, Zuzana (referee)
This theoretical-practical bachelor thesis focuses on dysfunction of the deep stabilisation system of the spine in female basketball players. Basketball is an unilateral sport depending on which hand we use to shoot or dribble, and therefore unilateral overloading occurs. This results in the development of muscle imbalances, painful syndromes, and a higher risk of injury. The theoretical part addresses several thematic areas important for understanding the issue. These include posture and its components, the deep stabilizing system of the spine, specifically its anatomy, functions, dysfunctions, and examination. Another area is a summary of developmental kinesiology and its most significant periods during childhood. Proper child development is the foundation of the Dynamic Neuromuscular Stabilization (DNS) concept included in the fourth thematic area. The fifth theme covers muscle imbalances, their types, and causes. The last section offers a comprehensive insight into basketball itself, analyzing its movement patterns, most stressed muscles, and common injuries resulting from this sport. The main goal of the practical part was to apply elements of DNS in the physiotherapeutic intervention for 5 young female basketball players with proven dysfunction of the deep stabilizing system of the spine....
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Western blot analysis of markers of endothelial dysfunction in mice aorta following high-fat diet administration
Střelcová, Ivana ; Němečková, Ivana (advisor) ; Vitverová, Barbora (referee)
7 2. ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Title of Thesis: Western blot analysis of markers of endothelial dysfunction in mice aorta following high-fat diet administration Supervisor: RNDr. Ivana Němečková, Ph.D. Author: Ivana Střelcová Background: High dietary fat intake is associated with increased risk of developing various metabolic diseases, including increased cardiovascular risk. The aim of this thesis was to investigate whether the administration of the high-fat diet containing 42 % kcal of fat affects expression of selected markers of endothelial dysfunction, the key mechanism in development of cardiovascular complications. Methods: In this thesis were evaluated changes in the expression of endothelial nitric oxide synthase (eNOS), endoglin (ENG), endothelin-1 (ET-1), intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1). An experimental group of male C57BL/6J mice aged 7 months was fed the high-fat diet containing 42% kcal of fat for 12 weeks. The control group was fed a standard laboratory diet. Western blot analysis followed by detection using a chemiluminescent reagent was used to evaluate the observed markers of endothelial dysfunction Results: The results show that there were no...
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Sexual harassment of nurses by patients
Laudová, Kristina ; Tomová, Šárka (advisor) ; Javůrková, Alena (referee)
The bachelor's thesis entitled "Sexual harassment of nurses by patients" discusses sexual harassment in the healthcare sector. Objectives: the purpose of the work is to determine the frequency and form of sexual harassment of nurses by patients, to determine the response of nurses and to compare sexual harassment of nurses in surgical and internal departments. In the theoretical part of the thesis, sexual harassment is defined according to international institutions. Sexual harassment in the healthcare sector, its recognition, risks, prevention and remedial solutions are further described. In the empirical part, the results of the own survey, statistical treatment of hypotheses and comparison of the results with other studies are presented. Methods: To achieve the aims of the work, a questionnaire survey was carried out, the target group of which was nurses from six teaching hospitals in the Czech Republic from internal or surgical clinics. 482 completed questionnaires were collected. Results: A third of the respondents answered that they had been sexually harassed by the patient, and in almost one hundred percent of the cases, the aggressor was a man. The frequency with which the respondents encountered sexual harassment was in the order of a month, about ten times a year. Disgust or anger (44%)...
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Differentiation of musculoskeletal disorders related to the nursing profession in inpatient and outpatient wards
Malinová, Lenka ; Nováková, Tereza (advisor) ; Maršáková, Kateřina (referee)
Title: Differentiation of work-related musculoskeletal disorders among nurses in inpatient and outpatient care units Objectives: The aim of this study was to determine a prevalence of work-related musculoskeletal disorders among nurses and compare localization of disorders among inpatient and outpatient care units nurses. Another goal of the study was to find a connection between WRMDs and occurring common factors. Methods: There was a questionnaire made to conduct this study. It was based on standardised Nordic musculoskeletal questionnaire (NMQ) and sent in both printed and electronic form to various healthcare facilities. The research group consisted of 146 respondents, mostly women, that work as a nurse. Results were processed from obtained data and the conclusions were determined. Results: In the last 12 months, 87 % of nurses suffered from work-related musculoskeletal disorders in at least one area of their body. The most commonly affected area was lower back and neck, both regions approximately 65 %. The biggest difference between nurses working in inpatient and outpatient care units was hand and wrist, where the results showed three times percentage of MS disorder occurence in the first group. Another difference was in lower back area, approximately 20 % less outpatient care units nurses...
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Quality of Life of People with Idiopathic Scoliosis after Surgical Treatment in the Czech Republic
Hušková, Pavlína ; Pavlů, Dagmar (advisor) ; Šteffl, Michal (referee)
Title: Quality of Life of People with Idiopathic Scoliosis after Surgical Treatment in the Czech Republic Objectives: The aim of the research part of the thesis was to evaluate the quality of life of individuals after surgery for idiopathic scoliosis in the Czech Republic on the basis of a questionnaire survey. Within the framework of the research, individuals with IS who had undergone surgical treatment of IS were contacted, but at the same time individuals with IS who had not undergone scoliosis surgery were contacted. Methods: The study population included individuals diagnosed with idiopathic scoliosis in the age range of 18-60 years. Another criterion for inclusion in the study was a minimum of two years after IS surgery. Two questionnaires were used to assess quality of life in these two groups of scoliotic patients with treatment different undergone: the Short Form 36 and the Scoliosis Research Society 22, which were distributed to individuals with IS for approximately eight months. Analysis of the obtained data and statistical evaluation of the results was performed using MS Excel software. Graphical representation of the results was performed using the Matplotlib library in the Python programming language. Results: According to the results of the SRS-22r questionnaire, operated individuals...
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Investigation of the acyltransferase RtxC interaction with the Kingella kingae toxin RtxA
Lichvárová, Michaela ; Osičková, Adriana (advisor) ; Černá, Věra (referee)
The bacterium Kingella kingae was first isolated in 1960 by microbiologist Elizabeth O. King and until recently, it was considered a rare cause of human disease. However, over the past 30 years, an increasing number of papers have shown that this bacterium is an important paediatric pathogen, mainly affecting children aged 6 months to 3 years, causing mainly septic arthritis, osteomyelitis, infective endocarditis, and bacteraemia. K. kingae displays a strong cytotoxic effect against a variety of host cell types, which is caused by the secreted cytolysin RtxA, a member of the RTX (Repeats in ToXin) family. RtxA binds to glycosylated structures of the host cell, subsequently inserts into its cytoplasmic membrane, and forms cation-selective pores, leading to disruption of ion homeostasis and lysis of the attacked cell. RtxA is produced as an inactive protoxin proRtxA. Its activation is mediated by the acyltransferase RtxC, which transfers acyl chains to conserved lysine residues K558 and K689 in the protoxin. It uses the acyl carrier protein ACP as the acyl donor. Currently, it is unclear how the RtxC, acyl-ACP, and proRtxA proteins interact with each other and which amino acid residues are responsible for these interactions. The aim of this thesis was to identify the residues responsible for these...
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Therapeutic significance of manual lymphatic drainage in lower limbs lipedema
SRBENÁ, Miloslava
This bachelor thesis deals with the issue of lower lipedema and the effect of manual lymphatic drainage and other regimen measures on the treatment of this disease. I have divided the theoretical part into 4 subchapters. The first subchapter introduces the topic of the thesis and discusses the morphology and physiology of the lymphatic system. The second subchapter deals with the anatomy of the lymphatic system. The following subchapter describes lipedema in detail. And the last subchapter in the theoretical part focuses on the treatment of the disease and other interventions. The practical part of the research was done in the form of qualitative research and case studies. I worked with five patients. The patients were under the professional supervision of Mrs. Mgr. Beránková, manual lymphatic drainage was performed once a week for three months and at the same time the probands followed the regimen measures, in which there were some differences in each of them. I evaluated the results by means of input and output kinesiological analysis, in which history, aspection, somatometry, palpation played a pivotal role. The main aim of the bachelor thesis was to analyze the effect of manual lymphatic drainage in patients with lower lipedema. In all probands there was a certain improvement in the physical properties of the skin called elasticity, tension disappeared, the feeling of heaviness, pain was eliminated and somatometric and goniometric examination also showed optimization. Of course, it all depended on the degree of lipedema and strict adherence to the regimen, i.e. dietary steps, training of the correct breathing stereotype, certain physical activity using an exercise unit to support the lymphatic system, compressive underwear, cold water hardening, brushing, gels with a warming or cooling effect.
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