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Needs of patients with age related macular degeneration
Houdková, Eliška ; Jirkovský, Daniel (advisor) ; Haluzíková, Jana (referee)
This bachelor's thesis deals with the area of specific needs in patients with age-related macular degeneration (AMD). Objectives: The aim of this thesis is to provide an overview of the changed needs of patients with AMD. Methods: To achieve the aim of the thesis, a questionnaire survey was conducted. The target group of the survey were patients undergoing anti-VEGF therapy at an unnamed eye clinic. A total of 70 questionnaires were included in the survey. Results: Based on the statistical evaluation of the data, it was found that AMD significantly affects the lives of respondents in many areas. The majority of respondents identified independence and self-sufficiency as the most affected aspect of their lives. More than half of the respondents (55.71 %) have problems with mobility and everyday activities. AMD also negatively affects the mental health of patients, 70 % of respondents are afraid of depression or anxiety. Respondents find meaning and fulfillment in life, especially in the areas of family and friends relationships, leisure activities, spiritual development, education, gratitude, and a positive attitude towards life. Conclusion: AMD has a complex impact on the lives of patients. It is important to provide patients with AMD with high-quality and accessible care that includes not only...
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Mechanisms responsible for sex bias in the prevalence of autoimmune diseases
Ráček, Vojtěch ; Daňková, Pavlína (advisor) ; Ciferská, Hana (referee)
Studies have shown that women typically have stronger immune responses than men. This can also make them more susceptible to autoimmune diseases, with females comprising 80% of autoimmune patients. In women, one X chromosome is epigenetically silenced through a process called X chromosome inactivation. However, some immune-related genes located on the X chromosome, such as TLR7 and CD40L, may escape this silencing process in certain cells or tissues. This can result in a sex-biased expression that contributes to the development and susceptibility of autoimmune diseases like lupus, rheumatoid arthritis, and Sjögren's syndrome. Another interesting factor that explains this sex bias is lncRNA XIST, which is a primary regulator of the inactivation process. It plays a crucial role in lupus development and creates an XIST ribonucleoprotein complex with proteins that help establish a repressed chromatin state on the inactivated chromosome. Importantly, many of these proteins are autoantigens in various autoimmune diseases and may serve as clinical markers for the diagnosis of these conditions. While microbiome and sexual hormones also play important roles, the focus remains primarily on XIST RNA and escape genes. Keywords: autoimmune disease, sex bias, X chromosome, escape genes, lncRNA XIST, sexual...
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Virus interaction with the cellular DNA damage response
Lemberková, Eva ; Šroller, Vojtěch (advisor) ; Kadlečková, Dominika (referee)
Maintenance of genomic integrity is an essential mechanism for every cell. Genomic integrity is disturbed by diverse exogenous or endogenous effects influencing the cell and causing damage of its DNA. Cellular mechanisms capable of fixing these disturbances in structure or sequence are indispensable because damaged genetic information can later cause expression of damaged proteins or inaccurate segregation of chromosomes to daughter cells. Therefore, many effective mechanisms for fixing wide range of types of DNA damage have evolved. This thesis focuses mainly on the eucaryotic MRN complex, which plays an important role in detection and repair of double strand breaks. Many viral families try to block these cellular repair mechanisms because they are activated soon after viral infection. One of the reasons for their activation is the resemblance of some viral genomes to the cellular DNA with double strand breaks. Thus, in many cases, the cell ends up inhibiting the life cycle of the virus by attempting to repair viral genomes. However, there are viruses that use cellular repair mechanisms for the replication of their genome, making these mechanisms essential for their own growth. Key words: DNA damage response, homologous recombination, non-homologous end-joining, MRN complex, Adenoviridae,...
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Helminth-host interactions on hormonal level
Lukesová, Hedvika ; Mikeš, Libor (advisor) ; Horák, Petr (referee)
Interactions between helminths and their hosts at the hormonal level result from their biochemical coevolution. Host hormones play an important role in the life of many helminths, and helminths are able to exploit them in some cases. For example, by expressing their own analogous hormone receptors that bind host hormones. Alternatively, by influencing the levels of host hormones, as certain hormonal environments may be (dis)advantageous for the helminths. This is the case, for example, of male mice infected with Taenia crassiceps, which undergo feminization as a result of infection. Some helminths can produce their own specific factors that influence host physiology, behaviour or appearance and may be analogs (orthologs) of host hormones. An example is the plerocercoid growth factor produced by Diphyllobothrium mansonoides, which behaves very much like a growth hormone in the host, although it differs in its primary structure. Some hormonal interactions can affect both helminth reproduction and the reproduction of their hosts. Examples of host-specific hormones that have been shown to be produced by helminths include, e.g., the ecdysteroid hormones - ecdysone and ecdysterone. A full understanding of the hormonal interactions between helminths and their hosts may lead us to find new treatments for...
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Updated summary of Tertiary relics in the vicinity of Pilsen
Trnka, Jan ; Sakala, Jakub (advisor) ; Pšenička, Josef (referee)
The aim of the bachelor thesis is to consolidate the knowledge from previous research on Tertiary relics in the vicinity of Pilsen, especially in the districts of Pilsen-City and Pilsen-North. Emphasis is placed mainly on sites that are important in terms of their extent or have provided identifiable samples of plant macrofossils or pollen in the past. In particular, these are the sites Dobříč/Jarov, Ejpovice/Kyšice and Horní Bříza - Modrý kříž. To a lesser extent, these are summaries of the manifestations of volcanism in the defined area. The thesis will be a possible starting point for a follow-up thesis, in which the student will process new findings of plant macrofossils from his own field research. Its further content will be a critical comparison of the individual reconstructions of the palaeochannel network and the determination of the approximate flow direction both for individual relics and the determination of the dominant drainage direction of the entire area.
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Effect of mitochondrially targeted senolytic agents on the development of respiratory diseases
Rennerová, Michaela ; Štemberková-Hubáčková, Soňa (advisor) ; Neckář, Jan (referee)
Aging is a complex biological process characterized by gradual decline in the physiological functions of cells and tissues, leading to the activation of a process known as cellular senescence. This results in the development of structural and functional changes in the organism accompanied by an increased risk of various diseases. Respiratory diseases are among the most common chronic conditions associated with the accumulation of senescent cells in the body. Current treatment for chronic respiratory diseases primarily focuses on symptom relief rather than addressing the primary cause of these conditions. Given that senescent cells play a significant role in the pathogenesis of respiratory diseases, the future of therapies lies in their elimination using senolytic agents. Despite promising results from some studies, current research is limited by the heterogeneity of senescent cells, which is reflected in their sensitivity to senolytic agents and the variability of therapeutic responses. Mitochondria play a central role in the development and maintenance of the senescent cell phenotype by regulating key processes such as energy metabolism and cellular signaling, regardless of the type of senescent cell. Thus, targeting mitochondria in senescent cells may represent a promising therapeutic strategy...
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Conflict prevention and de-escalation in pediatric nursing
Prosová, Iveta ; Raudenská, Jaroslava (advisor) ; Vlachová, Marie (referee)
Background: This paper focuses on conflict prevention and de-escalation in pediatric nursing. Aims: The theoretical aim of this thesis is to review relevant information on conflict prevention and de-escalation in paediatric nursing from the available Czech and international literature. The obtained knowledge is to be sorted, critically assessed and evaluated. The empirical aim of this thesis is to carry out quantitative research using a non-standardized questionnaire distributed distributed to nurses working in paediatric inpatient wards of Motol University Hospital, to statistically process and evaluate the obtained data and to formulate conclusions and recommendations on conflict prevention and de-escalation for practice based on the results of the questionnaire survey. Methodology: The research was conducted in the form of a non-standardized anonymous questionnaire of our own design, distributed to paediatric, general and practical nurses working in the paediatric wards at Motol Hospital. 144 correctly completed questionnaires are included in the final study. Results: The research survey revealed that although 72% of nurses consider education on conflict prevention and de-escalation important, only 21% of nurses have received such education in the last 5 years. Of the 68% of nurses who report...
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The importance of the Alps as a refugium in the Quarternary climate cycle with a focus on molluscs.
Gelnarová, Barbora ; Juřičková, Lucie (advisor) ; Coufal, Radovan (referee)
The Alps are a well-known glacial refugium, an important biodiversity hotspot and a centre of endemism for many species. This is due to their topological variability and the high heterogenity of their habitats, which thus provide a home for different species of organisms. In particular, the Eastern Alps represented an important glacial refugium and contributed to the survival of countless different species during the Quaternary climate cycle. This bachelor thesis is a literature review of mainly zoological publications that focus on the study of glacial refugia of terrestrial gastropods in the Alps. A large part of the thesis is devoted to the reasons for the considerable biodiversity, and especially the endemism, of this region. Another part deals with the postglacial dispersal spread of terrestrial gastropod species from their refugia. Key words: Alps, refugium, endemism, biodiversity, Quarternary, postglacional spread, molluscs, paleobiology
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Political trials of the 1950s: a case study
Svoboda, David ; Kuklík, Jan (advisor) ; Blažek, Lukáš (referee)
81 Political trials of the 1950s - a concrete case study (The Babice Case) Abstract The subject of the thesis "Political Trials of the 1950s - a Specific Case Study", subtitled The Babice Case, was a description of the application of the general tools of political persecution used in the Czechoslovak Republic in the 1950s to the specific case of the shooting at the Babice school and the subsequent political trials. Also significant is the aim of placing the political trials in Czechoslovakia in the context of the times and the international political situation, which played an important role in the Babice case. The first chapter of the thesis formulates the aims of the thesis and the background on which it is based. The second chapter of the thesis describes the significance of the political processes and the general background of their organisation. An important part of Chapter 2 of the thesis is a description of the legal framework for the conduct of political trials, focusing both on substantive aspects, such as the newly defined offences, and on procedural issues, especially the limitations on the rights of the accused. The legal biennium, an initiative aimed at changing the legal system to reflect the needs and ideology of the Communist Party, is also described as a significant phenomenon. The third...
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The importance of polyploidization in the evolution of grasses of the tribe Triticeae
Papoušek, Lukáš ; Urfus, Tomáš (advisor) ; Chrtek, Jindřich (referee)
Polyploidization, as a process of chromosome set multiplication, represents one of the most significant forces that shaped the evolution of angiosperms. Therefore, understanding the mechanisms of polyploidization is also important. It most often originates through unreduced gametes (less so through somatic polyploidization). Autopolyploids are the result of polyploidization within one species, or population, while the formation of allopolyploids is preceded by interspecific hybridization. Polyploidization also played a major role in the tribe Triticeae (Poaceae), which includes economically significant crops (wheat, rye, and barley). The taxonomy ofthe entire tribe, especially the generic concept, has been based on genomic concepts in recent decades. However, taxonomy is considerably complicated, particularly due to hybridization, or allopolyploidization. The result of allopolyploidization and even hybridization itself is a reticulate pattern, and it is not at all easy to determine the boundaries between taxa, even at the generic level. Triticum aestivum can be considered a model species for the study of allopolyploidy, both due to its economic significance and its origin through several rounds of hybridization events. Although allopolyploidy prevails, autopolyploidy can be encountered to a lesser...
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