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Vacuolar aspartic protease of Candida albicans
Hirko, Dominik ; Heidingsfeld, Olga (advisor) ; Dostál, Jiří (referee)
Aspartic proteases (APs) are crucial for diverse cellular processes. This thesis delves into the complexities of protein expression and characterization of vacuolar aspartic endoprotease Apr1p from Candida albicans, comparing it to its Saccharomyces cerevisiae ortholog, Pep4p. Recombinant expression of Apr1p in Escherichia coli yielded the inactive proenzyme, proApr1p. Extensive refolding efforts failed to produce mature, active Apr1p, suggesting a reliance on intricate cellular machinery or specific post-translational modifications for activation. Attempts to leverage vacuolar enzymes or cell lysates for proApr1p activation were unsuccessful, potentially due to the fragility of isolated vacuoles and the complex mixture of enzymes in cell lysates. Positive results emerged when Apr1p was expressed in S. cerevisiae, where fractionated cell lysates exhibited specific proteolytic activity at acidic pH after inhibiting serine and metalloproteases proteases. The eukaryotic system can probably produce active Apr1p. However, after preliminary small-scale experiments, upscaling of Apr1p expression in S. cerevisiae will be necessary in order to obtain sufficient amount of protein for further characterization. A reciprocal gene swap experiment, exchanging PEP4 in S. cerevisiae with APR1 and vice versa,...
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Acyclic Profinet Communication between Sinamics Converters and Simatic S7-1200 PLCs
Dostál, Jiří ; Janda, Karel (referee) ; Pásek, Jan (advisor)
This paper deals with acyclic communication of Siemens Simatic S7-1200 PLCs and Sinamics drives on the Profinet industrial bus. Various possibilites of communication of the devices in accordance to the PROFIdrive profile is presented. General Proxy FBs for acyclic read/write of the drive's parameters are implemented. Together with other two FBs, that make use of acyclic communication to read the drive's fault buffer and to adjust an absolute encoder, an application with visualization for a simple positioning and for a demonstration of the Proxy FBs functionality is presented using a demo box.
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Estimation of Company Value
Dostál, Jiří ; Busta, Karel (referee) ; Kocmanová, Alena (advisor)
The subject of this master’s thesis is estimation of company value. Theoretical part defines basic concepts such as value, categories of value, company, financial analysis and methods of valuation. The practical part deals with strategic analysis, financial analysis and estimation of real company market value by profit method of discounted cash flow.
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Flow Measurement of Hot Dusty Gas
Dostál, Jiří ; Štohl, Radek (referee) ; Šedivá, Soňa (advisor)
The bachelor thesis deals with the measurement of hot dusty gas. The introduction outlines the problems of measurement. Another part of the work contains a theoretical introduction to the measurement of gas flow. In successive chapters the basic principles and characteristics of suitable methods for measuring the hot dusty gases are described. Finally, the function blocks are implemented in the Simatic STEP7 for calculation of volume flow rate and blocks are tested on PLC.
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Search in Semantically-Enriched Texts
Dostál, Jiří ; Dytrych, Jaroslav (referee) ; Smrž, Pavel (advisor)
This thesis deals with searches within large-scale text data, which are enriched by semantic information, especially the highlighting of named entities. The thesis begins by analysing methods used for the indexing of partly structured text data and the methods used for searching. The main body of the thesis consists of the description and implementation of a query engine, which analyses queries and requirements on range and structure. This query is later converted into an MG4J search engine format and applies restrictions on the results. These results have been tested on time response, as well as on a number of results, which are then compared to older results obtained from English Wikipedia.
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Evulation of the Financial Situation in the Firm and Proposals to its Improvement
Dostál, Jiří ; Macálka, Jan (referee) ; Zinecker, Marek (advisor)
This bachelor´s thesis deal with financial analysis company Agrovykup, a.s., which produces feeds, do a business and transportation. There is used analysis absolute roadsings, distance roadsings, ratio roadsings and analysis system roadsings in work. By using those methodology is achieved purposes, namely evaluation of financial situation company Agrovykup, a.s. Contribution firm are constructive findings and recommendation for improvement financial situation by the help of which firm is able to achieve prosperity.
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HAWK controller data processing system
Dostál, Jiří ; Jirgl, Miroslav (referee) ; Kaczmarczyk, Václav (advisor)
This thesis deals with developing program components for collection of semantically labeled data from Honeywell's Hawk controller. The basic principles and capabilities of development using Niagara Framework, on which Hawk is based, are explained. Lastly, the specific components and external database application is described.
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Technological process simulation
Dostál, Jiří ; Veselý, Libor (referee) ; Malounek, Petr (advisor)
This paper deals with a design of an inverted pendulum model and its controller in Matlab/Simulink, a conversion of the model and its controller to the ANSI C language and its implementation to B&R PLC's. The source code for B&R PLC's is generated automatically by the B&R Automation Studio Target for Simulink library. The first part deals with the design of a mathematical model of the inverted pendulum and its simulation in Matlab/Simulink, followed by a design of a LQ controller for this model using Matlab functions and a description of the B&R Automation Studio Target for Simulink library. The last part of this paper deals with the implementation of the model and its controller into two separate B&R PLC's, which are networked with the Ethernet Powerlink industrial fieldbus and it also describes visualizations that were designed for operating the system.
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Flow Measurement of Hot Dusty Gas
Dostál, Jiří ; Štohl, Radek (referee) ; Šedivá, Soňa (advisor)
The bachelor thesis deals with the measurement of hot dusty gas. The introduction outlines the problems of measurement. Another part of the work contains a theoretical introduction to the measurement of gas flow. In successive chapters the basic principles and characteristics of suitable methods for measuring the hot dusty gases are described. Finally, the function blocks are implemented in the Simatic STEP7 for calculation of volume flow rate for the methods selected in the theoretical part and visualization in WinCC flexible.
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The issue of vitamin D in elite sport
Káchová, Nikola ; Dostal, Jiří (advisor) ; Kalendová, Barbora (referee)
Vitamin D is a fat-soluble vitamin that can be synthesized in the skin after exposure to sunlight or obtained through food or supplements. The aim of this study was to investigate the effect of 14 days of sun exposure in an area with higher levels of UV radiation (Dubai, United Arab Emirates) on vitamin D concentrations in a selected group of water slalom athletes and to further evaluate the changes in concentrations after a further 14 and 30 days after return. Data for the study were obtained by using four blood draws and comparing the average vitamin D concentration between the blood draws. Eleven elite athletes aged 18-35 years who were not taking vitamin D supplements participated in the study. The results showed that before the intervention, the athletes had average vitamin D levels lower (69.4 nmol/l) than the lower reference limit set at 75 nmol/l. Exposure to sunlight for 14 days resulted in a significant increase in vitamin D concentration, with an average increase of 10.8 nmol/l in the study group. Upon return to the Czech Republic, the vitamin D level increased slightly by an average of 0.7 nmol/l for 14 days, but this increase was not statistically significant compared to the previous sampling. After 30 days of stay, the vitamin D level decreased on average by 1.3 nmol/l. This result...
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