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Effect of erythropoietin on myocardial ischemic tolerance
Jindrová, Helena ; Kolář, František (advisor) ; Žurmanová, Jitka (referee)
Adaptation to chronic hypoxia increases myocardial resistance to acute ischemia/reperfusion (I/R) injury, similarly to application of exogenous erythropoietin (EPO). Nevertheless, it is not known if EPO induced by chronic hypoxia plays a role in its cardioprotective mechanism. The aim of this study was to find out if protective effect of exogenous EPO adds up to protection offered by chronic hypoxia. Adult male mice (ICR) were adapted to intermittent hypobaric hypoxia 8 hours per day, 5 days per week for 5 weeks. The degree of hypoxia corresponded to 7000 metres. Control animals were housed for the same time in normoxic environment. Resistance to I/R injury was assessed according to size of myocardial infarction induced by 45-min global ischemia and 1-h reperfusion of the heart in vitro. Animals were treated 24 h before the experiment with 200 or 5000 U/kg EPO. Treatment with 200 U/kg EPO was sufficient to significantly limit infarct size in normoxic animals (33,56 ± 2,93 % vs. 25,71 ± 2,29 %). Hypoxic adaptation decreased infarct area to 23,49 ± 2,30%, but additive effect of EPO in hypoxic group was not detected. The results indicate that exogenous EPO employs the same cardioprotective mechanisms as adaptation to chronic intermittent hypoxia. Preliminary results indicate that repeated application of EPO...
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Molecular mechanisms in diabetic embryopathy
Čerychová, Radka ; Pavlínková, Gabriela (advisor) ; Kolář, František (referee)
Diabetic embryopathy is one of many serious complications associated with diabetes. It is known that maternal diabetes increases the frequency of congenital defects up to ten times. The most common defects are cardiovascular and neural tube defects. Molecular mechanisms of diabetic embryopathy are still not known. This work contributes to elucidation of molecular processes leading to development of cardiovascular defects in diabetic embryopathy. This study is based on observation that maternal diabetes affects transcriptional regulation of hypoxia-inducible factor 1 (HIF-1) in developing embryo. To study the influence of maternal diabetes on HIF-1 signaling pathway, we used mouse model heterozygous for "knock-out" of Hif1α gene. Our analyses showed the negative combinational effects of maternal diabetes and Hif1α+/- genotype on embryonic development and increased risk of diabetic embryopathy. Histological analysis demonstrated the increased incidence of cardiovascular defects, particularly defects of interventricular septum and hypoplastic compact left ventricular wall in embryonic day (E) 14.5 Hif1α+/- embryos compared to wt littermates from the diabetic pregnancy. Using qPCR, we analyzed gene expression changes in the embryonic hearts at E9.5 and E10.5. We selected genes important for the...
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History of University Volleyball in Czechoslovakia and Czech Republic
Chadimová, Julie ; Waic, Marek (advisor) ; Kolář, František (referee)
Title: History of Czechoslovak and Czech University Volleyball Objectives: Analysis of university volleyball and its role in university sport in former Czechoslovakia. Importace of university volleyball in context of Czech and Czechoslovak volleyball history. Today situation. Methods: Heuristic. Analysis of primary and secondary sources and synthesis. (Inderict) Eyewitness evidence. source criticism: authenticity, historical reliability; chronological method Results: University volleyball participated extensively on creating the elite Czechoslovakia position in international volleyball in the first half of 20th century. The Czech volleyball top league depends over a long period on players out of university students. Keywords: university sport, university volleyball contests, organisation
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Revenues of the Organising Committees of the Summer Olympic Games
Žižka, Martin ; Šíma, Jan (advisor) ; Kolář, František (referee)
Title: Revenues of the Organising Committees of the Summer Olympic Games Objectives: The main objective of this work is an analysis of the main revenues of the particular Organising Committees of the Summer Olympic Games. The ticketing is primarily analyses in this work. The second objective is to adjust and calculate a mechanism for transfer of currency. Values in national currencies are converted to CZK 2010, these values reflect the true value of what would have been payable in 2010 if the Olympics Games were held in the Czech Republic. Methods: The work uses methods of secondary analysis of documents for the data collection. The basic aggregate consist of the Summer Olympic games held 1896-2008, the estimates from London (2012) and Prague (2016,2020) are add to comparison. Variables are: revenues of the OVOH, revenues from television rights, revenues from a sponsorship, revenues from the TOP, revenues from ticketing, the average price of the sold ticket, the most expensive and the cheapest tickets at the Opening ceremony and number of the total sold tickets. The convert mechanism is used for convert and calculated with the exchange rates, the purchasing power parities and the consumer price index. The following analyses apply for the evaluation of results: the trend analysis for monitoring the...
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Canoe sprint in the Czech Republic 2004 - 2008.
Pavlík, Matouš ; Bílý, Milan (advisor) ; Kolář, František (referee)
Title: Canoe sprint in the Czech Republic 2004 - 2008. Objectives: The aim of the thesis has been to draw up the history of the Czech sprint canoing between 2004 and 2008. Methods: Standard methods have been used in the thesis, especially the chronological method. The actual gaining of materials has been achieved through a combination of the direct, indirect and progressive methods. Results: The content of this work deals with the organizational and competition activities of sprint canoeists in the Czech Republic in the given period. Keywords: Kayak, canoe, canoe sprint, Czech Canoe Union, International Canoe Federation, European Canoe Association, history.
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History of the Czech fencing since its beginning till 1938
Čapek, Martin ; Waic, Marek (advisor) ; Kolář, František (referee)
Title: History of the Czech fencing since its beginning till 1938. Objectives: Analyze historical development of the Czech fencing since its beginning to 1938. I would like to pay attention to the development of the Czech fencing federation, the most important results, the World fencing championship in Piešťany and also one of the greatest Czech club ČŠK Riegel. Methods: Most of the information I got in the National Archives in Prague. Moreover I got the publications and copies of magazines in the library of fencing club ČŠK Riegel, where they have history of their club in sheets from year to year. I used yearbooks and annual reports, where were reported the most important moments in the history of the federation. I also used the publications received from the Czech fencing association and publications dedicated to the sport of fencing in general. Results: The result is a comprehensive history of the Czech fencing till 1938. Keywords: Fencing, history, Czech fencing federation.
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