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The Methodology of Evaluation of Air Quality Effect on Library and Archival Collections
Mašková, Ludmila ; Smolík, Jiří ; Ondráček, Jakub ; Součková, M. ; Paulusová, H. ; Bartl, B. ; Benešová, M. ; Ďurovič, M.
The aim of the project was a development of evaluation method for indoor air quality in libraries and archives, targeted at reduction of damages on archival collections caused by adverse effects of environment. The project included indoor/outdoor monitoring of particulate matter and gaseous pollutants at four libraries and archives representing different outdoor environments: Zlatá Koruna (rural area), Třeboň (small town), Teplice (industrial area), and Prague (large city). The adverse effects of deteriorated environment were studied by comparison of identical incunabules from these locations. Parallel to field studies the deterioration of paper caused by deposited dust particles was studied at laboratory conditions. In addition different methods of mechanical cleaning of paper were tested. Based on the results precautions reducing the adverse effects of outdoor air pollution were developed.
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Methodology of Selection of Means for Mechanical Cleaning of Dust Particles from Paper Surface
Benešová, M. ; Mašková, Ludmila
The aim of this work was to identify methods of mechanical cleaning of dust particles from the paper surface, which are suitable for use in the restoration practice. For this purpose 15 methods of mechanical cleaning were tested. The basic requirement was the applicability of the methodology for the most restorers. Therefore the methodology included only approaches which do not require any special equipment. The results showed that the type of paper has no effect to cleaning results and the main criterion for selection of means of treatment is the extent of soiling.
Fulltext: content.csg - PDF
Plný tet: Metodika-mechanické čištění - PDF
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The Changes of Body Temperature of Patients during a Medical Procedure in Total Anesthesia
BENEŠOVÁ, Monika
The first part of the present dissertation shows mechanisms of keeping of body temperature in an optimum range as well as it divides and evaluates range of body temperature. My dissertation also deals with the ways of measurement of body temperature, methods of measurement and tools which are neccessary for measurement of body temperature. Another part presents characteristics of aneshtesia its division, ways of application and tools which are used for total anesthesia. It is also described an observation of body temperature during total anesthesia, negative consequences of hypothermia, regulations of body temperature during total anesthesia and prevention and treatment of hypothermia. There are descriptions of targets and hypothesis in the second part. There were made five hypothesis. The third part shows methods of collecting data itself. They are results of stucture observation of changes of body temperature of patiens in total anesthesia during surgery.The fourth part are charts and graphs showing results of measurements of body temperature of our patients in total anesthesia as well as results of the questionnaires based on answers from asked nurses. There are particular hypothesis and their results in discussion part. Hypothesis H1 was proved. Hyphothesis H2 is not possible to be disproved. Hyphothesis H3 was not proved. Hyphothesis H4 was proved and hyphotesis H5 was proved. We can deduce from the results that active use of tools for keeping body temperature in a physiological range can prevent decrease of body temperature. It is clear from the questionnaires that nurses are aware of complications connected to hypothermia. They actively use tools for keeping body temperature in physiological range. There is a summary in the conclusion of the dessertation whether all estimated targets were accomplished. As a result of the dissertation we can assume that using of tools for keeping body temperature of total anasthesia patients should be a standard. There should be also a standard to measure body temperature during an operation in total anasthesia itself.
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In vitro expansion and activation of natural killer cells for cell therapy.
BENEŠOVÁ, Monika
NK cells are part of the non-specific immune response and are one of the main components of antitumor immunity. They do not need antigen stimul for thein activation but recognize damaged ( transformed ) cells by characteristic decreased expression of MHC I molecules. These natural killers become subjekt of many clinical studies based on the use of anti-tumor activity of NK cells for both solid tumors and in hemato - oncological diseases. The aim of this study was to find optimal conditions for in vitro expansion and activation of NK cells. NK cells were isolated from mononuclear cell fraction by magnetic separation and cultured in two types of media SCGM and X - VIVO 10 with the addition of interleukin-2 respectively OKT3 antibody. The influence of the mononuclear cells on proliferation of NK cells was tested. After a 6- day culture , the cells were passaged and growth of NK cells was determined using hematological analyzer and flow cytometry. Expression of the activation markers CD25 and CD336 (NKp44) was observed. All experiments were conducted under conditions of good manufacturing practice. Slightly higher gain of NK cells was observed in SCGM media without a significant differences in the other additives. Much higher number of NK cells was observed in culture with supporting irradiated mononuclear cells. There were no differences in cultures with autologous or allogeneic cells. We found that NK cells with higher proliferative potential express increasingly CD25, unlike the cells with decreased proliferation which had increased expression of CD336 marker. The work led to the definition of the optimal culture conditions for NK cells and became the basis for the further development of the medicinal product from in vitro activated NK cells.
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Hlavní překážky v integračním procesu cizinců ze třetích zemí v České republice - Studie: České Budějovice, Jihočeský kraj
BENEŠOVÁ, Markéta
This master thesis focuses on the actual problematic of contemporary migration phenomenon and its consequences for the Czech Republic. The aim of the theoretical part is to outline migration patterns in the Czech Republic since the end of the World War II up to the present-day situation, summarize the legislative framework which encompasses the legal stay of the foreigners in the Czech Republic and overview the actual integration Policy and its application. The paper examines in particular the conditions for the non EU nationals, in particular the people with Vietnamese or Ukrainian origin. The aim of the research part is to explore closely the above mentioned nationalities, their integration mechanisms and life conditions in the Czech Republic.
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School readiness
BENEŠOVÁ, Marcela
In the teoretical part of my bachelor paper I addressed the issues of school readiness and the overall development of a pre-school child needed for entering into primary school. I dealt specifically with the definition of the concepts of school maturity, preschool age and causes of school immaturity. In the practical part I used diagnostic tests, observations, interviews and projective method to develop a case study of selected preschoolers. The goal od my practical work was to assess selected aspects of school readiness.
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Vliv variability genomu na interakci lidského organismu a životního prostředí - ENVIRONGENOM
Ústav experimentální medicíny AV ČR, v.v.i., Praha ; Skorkovský, Jiří ; Benešová, Marie ; Novák, Jiří ; Tabashidze, Nana ; Švecová, Vlasta ; Špátová, Milada ; Solanský, Ivo ; Nováková, Zuzana ; Milcová, Alena ; Líbalová, Helena ; Dejmková, Eva ; Schmutzerová, Jana ; Topinka, Jan ; Rössner, Pavel ; Dostál, Miroslav ; Brdička, Radim ; Šrám, Radim
Zpráva obsahuje jednotlivé výstupy spojené s realizací projektu (články, přednášky). Je uvedeno zhodnocení znečistění ovzduší v Teplicích, Prachaticích a v Praze za období 1994-2009 a vyhodnocení vztahu mezi znečištěným ovzduším a nemocností předškolních dětí v okrese Teplice a Prachatice. Je připojena zpráva o zdravotním stavu Romů ve sledované oblasti.
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Vliv variability genomu na interakci lidského organismu a životního prostředí - ENVIRONGENOM
Ústav experimentální medicíny AV ČR, v.v.i., Praha ; Skorkovský, Jiří ; Benešová, Marie ; Novák, Jiří ; Tabashidze, Nana ; Švecová, Vlasta ; Špátová, Milada ; Solanský, Ivo ; Nováková, Zuzana ; Milcová, Alena ; Líbalová, Helena ; Dejmková, Eva ; Schmutzerová, Jana ; Topinka, Jan ; Rössner, Pavel ; Dostál, Miroslav ; Brdička, Radim ; Šrám, Radim
Zpráva obsahuje jednotlivé výstupy spojené s realizací projektu (články, přednášky). Je uvedeno zhodnocení znečistění ovzduší v Teplicích, Prachaticích a v Praze za období 1994-2009 a vyhodnocení vztahu mezi znečištěným ovzduším a nemocností předškolních dětí v okrese Teplice a Prachatice. Je připojena zpráva o zdravotním stavu Romů ve sledované oblasti.
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Vliv variability genomu na interakci lidského organismu a životního prostředí - ENVIRONGENOM
Ústav experimentální medicíny AV ČR, v.v.i., Praha ; Skorkovský, Jiří ; Benešová, Marie ; Novák, Jiří ; Tabashidze, Nana ; Švecová, Vlasta ; Špátová, Milada ; Solanský, Ivo ; Nováková, Zuzana ; Milcová, Alena ; Líbalová, Helena ; Dejmková, Eva ; Schmutzerová, Jana ; Topinka, Jan ; Rössner, Pavel ; Dostál, Miroslav ; Brdička, Radim ; Šrám, Radim
Projekt je rozpracován do následujících dílčích cílů: Sledování a analýza nemocnosti (infekční onemocnění horních i dolních dýchacích cest, alergie) vybrané skupiny cca 1000 dětí v modelových okresech Teplice a Prachatice do věku 10 let. , Stanovení PAU-DNA aduktů vzniklých v prenatálním období metodou ELISA u kohorty 1100 dětí z modelových okresů Teplice a Prachatice (DNA získaná při narození), Stanovení oxidačního poškození DNA v prenatálním období u kohorty 1 100 dětí z modelových okresů Teplice a Prachatice (DNA získaná při narození), Stanovení genetického polymorfismu čipovou technologií u kohorty 1100 dětí z modelových okresů Teplice a Prachatice (DNA získaná při narození), Zhodnocení vztahu genotypu novorozence k jeho postižení nitroděložní růstovou retardací (IUGR), a pozdější nemocnost těchto dětí s ohledem na stav ovzduší, Studium významu genotypu jedince, ovlivňujiícího interakce se znečištěným ovzduším, vzhledem k nemocnosti sledovaných dětí, Studium specifity skupin genů (metabolických drah) na tvorbu PAU-DNA aduktů a oxidační poškození, Analýza vztahu mezi expozicí PM2.5, k-PAU, PAU-DNA adukty, oxidačním poškozením DNA a genotypem, Zhodnocení významu PAU-DNA aduktů a oxidačního poškození DNA u dětí při narození jako indikátoru genetické zátěže jedince, vliv na nemocnost sledovaných dětí, Zhodnocení vývoje znečištěného ovzduší v Teplicích (AIM ČHMÚ) a Prachaticích (AIM MÚ Prachatice) do r. 2010, Doporučení interpretace získaných výsledků pro potřeby hodnocení rizika a v případě možnosti pro snížení negativních účinků znečištěného ovzduší na zdravotní stav dětí. Zpráva obsahuje především publikované výstupy projektu.
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