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Preparation and testing of silver immobilized nanoparticles for SERS spectroscopy of biomolecules
Molnár, Peter ; Procházka, Marek (advisor) ; Šmejkal, Petr (referee)
Main goal of this work was to develop methodology of immobilization of silver nanoparticles to silanized glass plates and employment of these substrates in biomolecular study using surface-enhanced Raman scattering (SERS). The suitable substrates for SERS spectroscopy, borohydride-reduced colloidal nanoparticles immobilized by mercapto- or aminosilane, have been found. Good SERS spectra of positively charged TMPyP porphyrin as well as of water insoluble TPP porphyrin (without interference of the solvent) have been obtained. Our results clearly show, that our substrates provide unique possibility how to measure SERS spectra of the porphyrins in his native free-base form (what is impossible directly from the colloid). Further experiments confirm that the porphyrins are protected against metalation due to the presence of the silane employed for the silver particles immobilization.
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Preparation and testing of silver immobilized nanoparticles for SERS spectroscopy of biomolecules
Molnár, Peter
Main goal of this work was to develop methodology of immobilization of silver nanoparticles to silanized glass plates and employment of these substrates in biomolecular study using surface-enhanced Raman scattering (SERS). The suitable substrates for SERS spectroscopy, borohydride-reduced colloidal nanoparticles immobilized by mercapto- or aminosilane, have been found. Good SERS spectra of positively charged TMPyP porphyrin as well as of water insoluble TPP porphyrin (without interference of the solvent) have been obtained. Our results clearly show, that our substrates provide unique possibility how to measure SERS spectra of the porphyrins in his native free-base form (what is impossible directly from the colloid). Further experiments confirm that the porphyrins are protected against metalation due to the presence of the silane employed for the silver particles immobilization.
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Damages for personal injury
Molnár, Peter ; Šustek, Petr (advisor) ; Salač, Josef (referee)
Damages for personal injury The diploma thesis focuses on the analysis and evaluation of the recodified legal regulation on damages for non-pecuniary loss arising from personal injury in the light of its comparison with the relevant English common law. On the background of the historical evolution of the method for assessing the amount of damages for non-pecuniary loss arising from personal injury, as well as through the comparison of Metodika Nejvyššího soudu k náhradě nemajetkové újmy při ublížení na zdraví (bolest a ztížení společenského uplatnění podle § 2958 občanského zákoníku) with Guidelines for the Assessment of General Damages in Personal Injury Cases, the diploma thesis arrives at a conclusion in favour of the development and application of the former, which it deems to have been a suitably chosen pre-emptive measure that would allow Czech legal practice to approach damages for non-pecuniary loss arising from personal injury in a consistent and proportionate fashion right from the moment of the coming into effect of Act No. 89/2012 Coll., the Civil Code, without having to overcome a period of relative legal uncertainty, during which standardised amounts of damages would have been developed through judicial practice, as was the case in the English common law. As regards the...
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Preparation and testing of silver immobilized nanoparticles for SERS spectroscopy of biomolecules
Molnár, Peter
Main goal of this work was to develop methodology of immobilization of silver nanoparticles to silanized glass plates and employment of these substrates in biomolecular study using surface-enhanced Raman scattering (SERS). The suitable substrates for SERS spectroscopy, borohydride-reduced colloidal nanoparticles immobilized by mercapto- or aminosilane, have been found. Good SERS spectra of positively charged TMPyP porphyrin as well as of water insoluble TPP porphyrin (without interference of the solvent) have been obtained. Our results clearly show, that our substrates provide unique possibility how to measure SERS spectra of the porphyrins in his native free-base form (what is impossible directly from the colloid). Further experiments confirm that the porphyrins are protected against metalation due to the presence of the silane employed for the silver particles immobilization.
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Preparation and testing of silver immobilized nanoparticles for SERS spectroscopy of biomolecules
Molnár, Peter ; Procházka, Marek (advisor) ; Šmejkal, Petr (referee)
Main goal of this work was to develop methodology of immobilization of silver nanoparticles to silanized glass plates and employment of these substrates in biomolecular study using surface-enhanced Raman scattering (SERS). The suitable substrates for SERS spectroscopy, borohydride-reduced colloidal nanoparticles immobilized by mercapto- or aminosilane, have been found. Good SERS spectra of positively charged TMPyP porphyrin as well as of water insoluble TPP porphyrin (without interference of the solvent) have been obtained. Our results clearly show, that our substrates provide unique possibility how to measure SERS spectra of the porphyrins in his native free-base form (what is impossible directly from the colloid). Further experiments confirm that the porphyrins are protected against metalation due to the presence of the silane employed for the silver particles immobilization.
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