Název: Separation of cyclic diadenosine diphosphorothioate and the diastereomers of its difluorinated derivative and estimation of binding constants of their complexes with 2-hydroxypropyl-β-cyclodextrin by affinity capillary electrophoresis
Autoři: Štěpánová, Sille ; Břehová, Petra ; Kašička, Václav
Typ dokumentu: Příspěvky z konference
Konference/Akce: Advances in Chromatography and Electrophoresis & Chiranal 2024, Olomouc (CZ), 20240617
Rok: 2024
Jazyk: eng
Abstrakt: Cyclic dinucleotides (CDNs) are known to activate the stimulator of interferon genes (STING), a protein that is a member of the cyclic-guanosine-adenosine synthase-STING signaling pathway and that is important for the innate immune response in eukaryotic cells. CDNs contain two ribonucleoside monophosphates linked via 3′–5′ or 2′–5′ phosphodiester bonds. Many therapeutic oligonucleotides contain phosphorothioate modification. The aim of this work was to develop a new affinity capillary electrophoresis (ACE) method for the separation of a potential anticancer drug, 2′,3′-cyclic diadenosine diphosphorothioate (Rp, Rp) (ADU-S100), and three recently synthesized diastereomers of its difluorinated derivative, 3′,3′-cyclic di(2′-fluoro,2′-deoxyadenosine phosphorothioate) [1] (see Fig. 1) using native (α-, β-, and γ-cyclodextrins (CDs)) or modified CDs (2-hydroxypropyl-β-CD (HP-β-CD) and 2-hydroxypropyl-γ-CD (HP-γ-CD)) as chiral selectors. In addition, the background electrolyte (BGE) composition and the concentration of the suitable CD should be optimized. Moreover, ACE should be applied for the estimation of the average apparent binding constants of the complexes of the analyzed CDNs with the chiral selector providing best separation.
Klíčová slova: affinity capillary electrophoresis; binding constant; cyclic dinucleotides
Zdrojový dokument: Advances in Chromatography and Electrophoresis & Chiranal 2024, ISBN 978-80-244-6478-7

Instituce: Ústav organické chemie a biochemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: https://doivup.upol.cz/pdfs/doi/9900/06/7500.pdf
Původní záznam: https://hdl.handle.net/11104/0365819

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-678767


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav organické chemie a biochemie
Konferenční materiály > Příspěvky z konference