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Original title:
Chiral separation of ketamine and its metabolites from rat plasma samples using partial filling CE-ESI/MS
Authors: Konášová, Renáta ; Koval, Dušan ; Tůma, P. ; Kašička, Václav
Document type: Papers
Conference/Event: Advances in Chromatography and Electrophoresis & Chiranal 2024, Olomouc (CZ), 20240617
Year: 2024
Language: eng
Abstract: Ketamine is a chiral drug used for a nearly 60 years to induce and maintain anaesthesia. In addition, ketamine and especially its metabolites act as antidepressants. For that reason, the mechanisms of action of these species have been studied. Different effects of enantiomers of ketamine and its metabolites were observed. S-ketamine has about twice the analgesic potency\nthan the clinically used racemic mixture [1] and is supposed to be responsible for high abuse liability of ketamine [2]. The metabolism of R-ketamine to 2R,6R-hydroxynorketamine is essential for its antidepressant effect [3]. Ketamine and its main metabolites were successfully separated by capillary zone electrophoresis (CZE) [4] but a complete separation of enantiomers of ketamine (Ket) and its main metabolites, norketamine (NK), dehydronorketamine (DHNK) and hydroxynorketamine (HNK), has not yet been achieved. The recently developed LC-MS method requires one achiral and two chiral columns for separation of six enantiomers [5]. Therefore, the aim of this work was to develop a new CZE method that could separate the enantiomers of all above species in a single run.
Keywords: capillary electrophoresis; ketamine; mass spectrometry
Host item entry: Advances in Chromatography and Electrophoresis & Chiranal 2024, ISBN 978-80-244-6478-7
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: https://doivup.upol.cz/pdfs/doi/9900/06/7500.pdf
Original record: https://hdl.handle.net/11104/0365812
Permalink: http://www.nusl.cz/ntk/nusl-678764
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Authors: Konášová, Renáta ; Koval, Dušan ; Tůma, P. ; Kašička, Václav
Document type: Papers
Conference/Event: Advances in Chromatography and Electrophoresis & Chiranal 2024, Olomouc (CZ), 20240617
Year: 2024
Language: eng
Abstract: Ketamine is a chiral drug used for a nearly 60 years to induce and maintain anaesthesia. In addition, ketamine and especially its metabolites act as antidepressants. For that reason, the mechanisms of action of these species have been studied. Different effects of enantiomers of ketamine and its metabolites were observed. S-ketamine has about twice the analgesic potency\nthan the clinically used racemic mixture [1] and is supposed to be responsible for high abuse liability of ketamine [2]. The metabolism of R-ketamine to 2R,6R-hydroxynorketamine is essential for its antidepressant effect [3]. Ketamine and its main metabolites were successfully separated by capillary zone electrophoresis (CZE) [4] but a complete separation of enantiomers of ketamine (Ket) and its main metabolites, norketamine (NK), dehydronorketamine (DHNK) and hydroxynorketamine (HNK), has not yet been achieved. The recently developed LC-MS method requires one achiral and two chiral columns for separation of six enantiomers [5]. Therefore, the aim of this work was to develop a new CZE method that could separate the enantiomers of all above species in a single run.
Keywords: capillary electrophoresis; ketamine; mass spectrometry
Host item entry: Advances in Chromatography and Electrophoresis & Chiranal 2024, ISBN 978-80-244-6478-7
Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: https://doivup.upol.cz/pdfs/doi/9900/06/7500.pdf
Original record: https://hdl.handle.net/11104/0365812
Permalink: http://www.nusl.cz/ntk/nusl-678764
The record appears in these collections:
Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers
Record created 2025-04-20, last modified 2025-05-03