Název: Chiral separation of ketamine and its metabolites from rat plasma samples using partial filling CE-ESI/MS
Autoři: Konášová, Renáta ; Koval, Dušan ; Tůma, P. ; Kašička, Václav
Typ dokumentu: Příspěvky z konference
Konference/Akce: Advances in Chromatography and Electrophoresis & Chiranal 2024, Olomouc (CZ), 20240617
Rok: 2024
Jazyk: eng
Abstrakt: Ketamine is a chiral drug used for a nearly 60 years to induce and maintain anaesthesia. In addition, ketamine and especially its metabolites act as antidepressants. For that reason, the mechanisms of action of these species have been studied. Different effects of enantiomers of ketamine and its metabolites were observed. S-ketamine has about twice the analgesic potency\nthan the clinically used racemic mixture [1] and is supposed to be responsible for high abuse liability of ketamine [2]. The metabolism of R-ketamine to 2R,6R-hydroxynorketamine is essential for its antidepressant effect [3]. Ketamine and its main metabolites were successfully separated by capillary zone electrophoresis (CZE) [4] but a complete separation of enantiomers of ketamine (Ket) and its main metabolites, norketamine (NK), dehydronorketamine (DHNK) and hydroxynorketamine (HNK), has not yet been achieved. The recently developed LC-MS method requires one achiral and two chiral columns for separation of six enantiomers [5]. Therefore, the aim of this work was to develop a new CZE method that could separate the enantiomers of all above species in a single run.
Klíčová slova: capillary electrophoresis; ketamine; mass spectrometry
Zdrojový dokument: Advances in Chromatography and Electrophoresis & Chiranal 2024, ISBN 978-80-244-6478-7

Instituce: Ústav organické chemie a biochemie AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: https://doivup.upol.cz/pdfs/doi/9900/06/7500.pdf
Původní záznam: https://hdl.handle.net/11104/0365812

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-678764


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav organické chemie a biochemie
Konferenční materiály > Příspěvky z konference