Molekulární profilování k odhalení nových signatur u nádorových onemocnění

Matyašovská, Natália

11 Summary ENG While reliable biomarkers for personalized management of pancreatic neuroendocrine tumors (PanNETs) remains limited, transcriptomic profiling of microRNAs (miRNAs) and mRNAs has shown promise in segregating PanNET subtypes and predicting treatment response. Since miRNAs are key post-transcriptional regulators of mRNA expression, integrating data on dysregulated miRNAs and mRNAs could provide critical insights into the molecular pathogenesis of PanNETs. This study aimed to investigate the role of mRNA and miRNA expression in the molecular pathogenesis, clinical presentation, and prognosis of PanNETs compared to non- diseased controls. RNA sequencing was performed to identify differentially expressed mRNAs, with a focused analysis on clinicopathological biomarkers. PanNET samples were also classified into distinct molecular subtypes based on their transcriptomic profiles. miRNA sequencing was conducted to detect differentially expressed miRNAs, which were validated using quantitative real-time PCR and public datasets. An integrative analysis of miRNA- mRNA interactions was then performed to uncover regulatory networks and functional pathways. The results revealed significant dysregulation, with 715 upregulated and 702 downregulated genes identified in PanNETs. Pathway analysis provided insights...

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