Original title: Pokročilá mikroskopie ve výzkumu koloidních systémů
Translated title: Advanced microscopy in the research of colloidal systems
Authors: Valovič, Stela ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
Document type: Bachelor's theses
Year: 2017
Language: cze
Publisher: Vysoké učení technické v Brně. Fakulta chemická
Abstract: [cze] [eng]
Táto bakalárska práca je zameraná na dvojfarebnú fluorescenčnú kros-korelačnú spektroskopiu. Ako vhodná kombinácia sond pre túto metódu bola navrhnutá kombinácia perylen + nílska červeň, perylen + DiD a DiO + DiD. Na overenie funkčnosti FCCS boli použité tetraspecky. Samotné meranie bolo prevedené v micelárnych roztokoch tenzidov, ktoré sa však ukázali ako nevhodné pre túto metódu. Následne boli pomocou tejto metódy merané lipozomy, ktoré mali v dvojvrstve fosfolipidov zabudované fluorescenčné sondy DiO a DiD. V lipozomoch bola uspečne namerana kros-korelácia. This bachelor thesis is focused on dual-colour fluorescence cross-correlation spectroscopy. As a suitable combinations of fluorescenct dyes were designated this combinations: perylene + nile red, perylene + DiD and DiO + DiD. The funtionality of this method was veried by tetraspeck. The experiment of FCCS was performed on micellar surfactants solitions which proved to be unsuitable for this method. Consequently, liposomes with DiD and DiO incorporated in phospholipid bilayer were measured. The cross-correlation in liposomes was succesfuly measured.
Keywords: DiD; DiO; dual-colour fluorescence cross-correlation spectroscopy; fluorescence; liposomes; nile red; surfactants; DiD; DiO; fluorescencia; fluorescenčná kros-korelačná spektroskopia; lipozomy; nílska červeň; perylen; tenzidy

Institution: Brno University of Technology (web)
Document availability information: Fulltext is available in the Brno University of Technology Digital Library.
Original record: http://hdl.handle.net/11012/65398

Permalink: http://www.nusl.cz/ntk/nusl-589622


The record appears in these collections:
Universities and colleges > Public universities > Brno University of Technology
Academic theses (ETDs) > Bachelor's theses