Original title:
Redox labelling of nucleic acids for analyzing nucleotide sequences and monitoring DNA-protein interactions
Authors:
Fojta, Miroslav ;
Havran, Luděk ;
Horáková Brázdilová, Petra ;
Pivoňková, Hana ;
Kostečka, Pavel ;
Macíčková-Cahová, Hana ;
Raindlová, Veronika ;
Vrábel, Milan ;
Hocek, Michal
Document type: Papers
Conference/Event: Chemistry of Nucleic Acid Components /15./ , Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Year:
2011
Language:
eng
Abstract:
Nucleobase labelling of DNA for electrochemical sensing was attained through chemical modification of thymine bases with osmium tetroxide in the presence of nitrogenous ligands, or via enzymatic incorporation of nucleotide conjugates with redox-active moieties using labelled deoxynucleoside triphosphates. DNA hybridization, primer extension and PCR techniques were used for sequence-specific DNA assays. Tail-labelled DNA substrates were applied to monitor DNA binding by tumour suppressor p53 protein.
Keywords:
DNA ;
electrochemistry ;
redox labeling
Project no.: CEZ:AV0Z40550506 (
CEP ),
CEZ:AV0Z50040507 (
CEP ),
CEZ:AV0Z50040702 (
CEP ),
LC512 (
CEP ),
LC06035 (
CEP ),
IAA400040901 (
CEP ),
IAA400040903 (
CEP ),
GA203/09/0317 (
CEP ),
GPP206/11/P739 (
CEP )
Funding provider: GA MŠk, GA MŠk, GA AV ČR, GA AV ČR, GA ČR, GA AV ČR
Host item entry: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(
web )
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0198897
Permalink: http://www.nusl.cz/ntk/nusl-55849
The record appears in these collections: Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry Conference materials > Papers