Název:
Synthesis of nucleosides and nucleoside triphosphates bearing anthraquinone substituents as redox probes and their enzymatic incorporation to DNA
Autoři:
Balintová, Jana ; Havran, Luděk ; Fojta, Miroslav ; Hocek, Michal Typ dokumentu: Příspěvky z konference Konference/Akce: Chemistry of Nucleic Acid Components /15./, Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Rok:
2011
Jazyk:
eng
Abstrakt: Modified 2′-deoxynucleosides and nucleoside triphosphates (dNTPs) bearing anthraquinone (AQ) via acetylene or propargylcarbamoyl linkers were prepared by single-step Sonogashira cross-coupling reactions halogenated nucleosides (7-iodo-7-deaza-2′-deoxyadenosine and 5-iodo-2′-deoxycytidine) or dNTPs with N-(2-propynyl)-anthraquinone carboxamide and 2-ethynylantraquinone. Polymerase incorporation of the AQ-labelled dNTPs into DNA has also been studied. Square-wave voltammetry of the AQ-labelled nucleosides and nucleotides showed one reversible peak at –0.5 V.
Klíčová slova:
anthraquinone; DNA; nucleosides Číslo projektu: CEZ:AV0Z40550506 (CEP), LC512 (CEP), GA203/09/0317 (CEP), 1M0508 (CEP), IAA400040901 (CEP) Poskytovatel projektu: GA MŠk, GA ČR, GA MŠk, GA AV ČR Zdrojový dokument: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1
Instituce: Ústav organické chemie a biochemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0198836