Original title: Polymerase construction of base-modified DNA for chemical biology
Authors: Hocek, Michal ; Macíčková-Cahová, Hana ; Kielkowski, Pavel ; Raindlová, Veronika ; Kalachová, Lubica ; Riedl, Jan ; Balintová, Jana ; Ménová, Petra
Document type: Papers
Conference/Event: Chemistry of Nucleic Acid Components /15./, Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Year: 2011
Language: eng
Abstract: An efficient two-step methodology for construction of base-modified DNA was developed based on aqueous-phase cross-coupling reactions of halogenated nucleoside triphosphates (dNTPs) followed by polymerase incorporation. A number of diverse chemical modifications have been successfully incorporated in this way for the use in chemical biology (modulation of cleavage by restriction enzymes, interactions with diverse DNA-binding proteins and bioconjugations).
Keywords: DNA; nucleotides; polymerases
Project no.: CEZ:AV0Z40550506 (CEP), LC512 (CEP), IAA400040901 (CEP), GA203/09/0317 (CEP)
Funding provider: GA MŠk, GA AV ČR, GA ČR
Host item entry: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0198819

Permalink: http://www.nusl.cz/ntk/nusl-55824


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers