Název:
Identifikace a sekvenování genomu nového viru infikujícího vojtěšku
Překlad názvu:
Identification and sequencing genom of a new virus infecting lucerne
Autoři:
BEČKOVÁ, Martina Typ dokumentu: Diplomové práce
Rok:
2010
Jazyk:
cze
Abstrakt: Samples of lucerne plants characteristic with local necrosic lesions, leave malformation and yellow spots on leaves were investigated with transmission electron microscopy. Virus particles observed there were filamentous ones of 600 to 700 nm long. Nucleic acid was isolated, transcribed and amplified using PCR. Genus-specific primers were designed based on reverse genetics from the highly conserved genes for carlaviruses, potexviruses and potyviruses. Successful amplification with carlavirus-specific primers, sequencing and comparison with sequences in GenBank database revealed presence of a carlavirus. This was later identified by nucleotide sequence comparison as a new isolate V4 of Alfalfa latent virus. Specific primers for isolate V4 were designed in a coat protein position. Half of the genom of this virus was obtained with PCR and PCR modified amplifications and compared with sequences of Alfalfa latent virus and Pea streak virus from GenBank.
Klíčová slova:
Alfalfa latent virus; carlavirus; identifikace viru; izolát V4; Medicago sativa; Pea streak virus; sekvenace; vojtěška; Alfalfa latent virus; carlavirus; isolate V4; lucerne; Medicago sativa; Pea streak virus; sequencing; virus identification Citace: BEČKOVÁ, Martina. Identifikace a sekvenování genomu nového viru infikujícího vojtěšku. České Budějovice, 2010. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Instituce: Jihočeská univerzita v Českých Budějovicích
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