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Original title:
Identifikace a sekvenování genomu nového viru infikujícího vojtěšku
Translated title: Identification and sequencing genom of a new virus infecting lucerne
Authors: BEČKOVÁ, Martina
Document type: Master’s theses
Year: 2010
Language: cze
Abstract: Samples of lucerne plants characteristic with local necrosic lesions, leave malformation and yellow spots on leaves were investigated with transmission electron microscopy. Virus particles observed there were filamentous ones of 600 to 700 nm long. Nucleic acid was isolated, transcribed and amplified using PCR. Genus-specific primers were designed based on reverse genetics from the highly conserved genes for carlaviruses, potexviruses and potyviruses. Successful amplification with carlavirus-specific primers, sequencing and comparison with sequences in GenBank database revealed presence of a carlavirus. This was later identified by nucleotide sequence comparison as a new isolate V4 of Alfalfa latent virus. Specific primers for isolate V4 were designed in a coat protein position. Half of the genom of this virus was obtained with PCR and PCR modified amplifications and compared with sequences of Alfalfa latent virus and Pea streak virus from GenBank.
Keywords: Alfalfa latent virus; carlavirus; isolate V4; lucerne; Medicago sativa; Pea streak virus; sequencing; virus identification; Alfalfa latent virus; carlavirus; identifikace viru; izolát V4; Medicago sativa; Pea streak virus; sekvenace; vojtěška
Citation: BEČKOVÁ, Martina. Identifikace a sekvenování genomu nového viru infikujícího vojtěšku. České Budějovice, 2010. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/13533
Permalink: http://www.nusl.cz/ntk/nusl-53148
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Translated title: Identification and sequencing genom of a new virus infecting lucerne
Authors: BEČKOVÁ, Martina
Document type: Master’s theses
Year: 2010
Language: cze
Abstract: Samples of lucerne plants characteristic with local necrosic lesions, leave malformation and yellow spots on leaves were investigated with transmission electron microscopy. Virus particles observed there were filamentous ones of 600 to 700 nm long. Nucleic acid was isolated, transcribed and amplified using PCR. Genus-specific primers were designed based on reverse genetics from the highly conserved genes for carlaviruses, potexviruses and potyviruses. Successful amplification with carlavirus-specific primers, sequencing and comparison with sequences in GenBank database revealed presence of a carlavirus. This was later identified by nucleotide sequence comparison as a new isolate V4 of Alfalfa latent virus. Specific primers for isolate V4 were designed in a coat protein position. Half of the genom of this virus was obtained with PCR and PCR modified amplifications and compared with sequences of Alfalfa latent virus and Pea streak virus from GenBank.
Keywords: Alfalfa latent virus; carlavirus; isolate V4; lucerne; Medicago sativa; Pea streak virus; sequencing; virus identification; Alfalfa latent virus; carlavirus; identifikace viru; izolát V4; Medicago sativa; Pea streak virus; sekvenace; vojtěška
Citation: BEČKOVÁ, Martina. Identifikace a sekvenování genomu nového viru infikujícího vojtěšku. České Budějovice, 2010. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/13533
Permalink: http://www.nusl.cz/ntk/nusl-53148
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Record created 2011-08-22, last modified 2023-01-15