Hormonální synchronizace fetálních cirkadiánních hodin v suprachiasmatických jádrech

Kapsdorferová, Viktória

Rhythmic non-light maternal signals, such as various behavioral, neurohumoral or metabolic factors, may play a key role in synchronization of the fetal circadian clock. This diploma thesis focuses more closely on the possible role of leptin and dopamine in the hormonal synchronization of the fetal central clock resides in the suprachiasmatic nuclei of the hypothalamus. A transgenic mouse fetus, which has luciferase fused to the clock gene Period 2, was used as an experimental model. This allowed us to monitor the operation of the fetal central clock in real time by recording the bioluminescence of tissue explants. Exogenous administration of dopamine during the decline in bioluminescence at normalized circadian time 15 induces larger phase delays compared to vehicle. Leptin, on the other hand, can participate in the development of spontaneous rhythms by stabilizing the integrity of the circadian rhythm, manifested by slowing down the rhythm dampening. At the level of individual cellular oscillators, significant regional differences in amplitude and period were not observed using bioluminescence microscopy. However, a slight dispersion of cell phases was observed after administration of dopamine during decreasing bioluminescence. In the case of leptin, there was a prolongation of period of the cell...

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