Fotoafinitní spojování biomolekul

Masárová, Petra

Photoclick chemistry represents a class of photo-activated click reactions generally characterised by precise spatial and temporal control, high selectivity, and quantitative yields. The following thesis will focus on several classes of light triggered click reactions and attempt to outline their chemistry in reversible nucleophilic addition, irreversible cycloaddition and Diels-Alder reactions initiated upon irradiation of photoactive precursors. The absorption of such precursors can be shifted towards visible light by derivatization of their chromophoric moieties, which allows for a non-harmful excitation in vivo and therefore offers promising prospects in biological applicability. The importance of click photochemistry resides in a remarkable selectivity that enables reactions to take place in highly complex, non-controlled biological environments. The photo-inducible precursors can be conjugated with various moieties of biological significance - a method most notably used in photoaffinity labelling. Considerable efforts have been made to explore the reactivity of photoactivatable compounds towards biological nucleophiles in various spheres of applied research which will all be discussed and summarised.

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