Název:
Analýza vybraných nutraceutik pomocí kapalinové chromatografie
Překlad názvu:
Analysis of selected nutraceuticals by liquid chromatography
Autoři:
Ibrahim, Laureen Reda Youssef ; Jenčo, Jaroslav (vedoucí práce) ; Opletal, Lubomír (oponent) Typ dokumentu: Diplomové práce
Rok:
2023
Jazyk:
eng
Abstrakt: 39 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Pharmacognosy and Pharmaceutical Botany Candidate: Laureen Reda Youssef Ibrahim Supervisor: RNDr. Jaroslav Jenčo, Ph.D. Title of dissertation thesis: Analysis of selected nutraceuticals by liquid chromatography The extract obtained pellets of Humulus Lupulus L. var Columbus was subjected to a phytochemical study and isolation of its bitter acid constituents. The summary extract was separated into individual fractions by liquid-liquid extraction. After performing HPTLC, GC-MS, and LC-MS analysis, the fraction HL-Hex B was selected and purified further by preparative chromatography. A monocomponent pale yellow solid substance of lupulone was obtained after the final preparative separation. Based on instrumental analysis by reversed phase HPLC with core-shell particles, the analyte was compared to the extract of dietary supplement HopsVital where the presence of bitter acids in the supplement was shown. Key words: nutraceuticals, high performance liquid chromatography, sample preparation, modern stationary phases
Klíčová slova:
moderní stqacionární fáze; nutraceutika; vysokoúčinná kapalinová chromatografie; úprava vzorek; high performance liquid chromatography; modern stationary phases; nutraceuticals; sample preparation