Název:
Detailed Investigation of Human Galectins -1 and -3 by employing selectively deoxyfluorinated N-acetyllactosamines
Autoři:
Kurfiřt, Martin Typ dokumentu: Příspěvky z konference Konference/Akce: Bažant postraguate conference 2023, Prague (CZ), 20230531
Rok:
2023
Jazyk:
eng
Abstrakt: This work is focused on a detailed investigation of supramolecular recognition events between human galectins -1 and -3 and monodeoxyfluorinated N-acetyllactosamine probes, which were previously prepared in our laboratory. The recognition was studied using a combination of X-ray crystallography, isothermal titration calorimetry and several advanced NMR techniques, such as 19F T2-filter, 1H–1H STD, 1H–15N CSP or 19F EXSY. These techniques provided insight into the thermodynamics and kinetics of recognition and enabled us to uncover of subtle differences between both investigated galectins. Such differences could be potentially exploited in the selective targeting of individual galectins with important implications for the galectins-focused\npharmaceutic industry.
Klíčová slova:
galectins; NMR techniques; pharmaceutic industry Číslo projektu: GA23-06115S (CEP), LTC20052, CA18103 Poskytovatel projektu: GA ČR, GA MŠk Zdrojový dokument: Proceedings of Abstracts, ISBN 978-80-86186-29-0
Práva: Dílo je chráněno podle autorského zákona č. 121/2000 Sb.