Název: Differentiation of mesenchymal stem cells on fibrin assemblies supported by immobilized growth factors FGF2 and VEGF
Autoři: Musílková, Jana ; Filová, Elena ; Kaplan, Ondřej ; Bačáková, Lucie
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Conference on Nanomaterials - Research & Application /13./ NANOCON, Brno (CZ), 20211020
Rok: 2021
Jazyk: eng
Abstrakt: Bioartificial heart valves and vascular grafts prepared from decellularized tissues could be recellularized with bone marrow-derived mesenchymal stem cells (MSCs) that are able to differentiate into both smooth muscle cells and endothelial cells. MSCs differentiation is facilitated by sustained release of growth factors. In our study assemblies based on fibrin, fibrin with heparin, fibrin with adsorbed or covalently-immobilized vascular endothelial growth factor A165 (VEGF) or basic fibroblast growth factor (FGF-2) via binding to heparin attached to fibrin have been prepared and were evaluated for their stimulation of MSCs differentiation. We estimated the mRNA expression of endothelial marker CD31 (PECAM1), smooth muscle marker α-actin (ACTA2), osteoblast markers osteocalcin (BGLAP) and alkaline phosphatase (ALP). The gene expression was estimated using RT-PCR on days 1, 7 and 21 after seeding. The cell morphology and viability was evaluated by LIVE/DEAD staining. VEGF, both adsorbed and covalently bound, increased significantly the expression of smooth muscle marker α-actin. The mRNA expression of ACTA2 on day 7 and 21 raised more than 200 times in comparison to control samples (undifferentiated cells before seeding). The ACTA2 gene expression significantly exceeded the expression of all other evaluated genes at all time intervals. Moreover, on day 21, the late smooth muscle marker desmin (DES) was steeply rising in cells cultivated on assemblies containing heparin and covalently bound VEGF. The expression of osteocalcin was minimal. We conclude that fibrin assembly containing covalently bound VEGF is the most convenient for MSCs differentiation towards smooth muscle cells.
Klíčová slova: differentiation; fibrin assemblies; growth factors; smooth muscle cells; stem cells
Číslo projektu: NU20-08-00208
Poskytovatel projektu: GA MZd
Zdrojový dokument: NANOCON 2021 - Conference proceedings, ISBN 978-80-88365-00-6, ISSN 2694-930X

Instituce: Fyziologický ústav AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný na externích webových stránkách.
Externí umístění souboru: https://www.confer.cz/nanocon/2021/read/4360-differentiation-of-mesenchymal-stem-cells-on-fibrin-assemblies-supported-by-immobilized-growth-factors-fgf2-and-vegf.pdf
Původní záznam: https://hdl.handle.net/11104/0338712

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-519928


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Fyziologický ústav
Konferenční materiály > Příspěvky z konference