Psychiatricky-relevantní postižení myších modelů spinocerebelární ataxie

Tichánek, Filip

Spinocerebellar ataxias (SCAs) constitute a heterogeneous group of diseases characterized by the dysfunction of the cerebellum and disturbed coordination of movements. Aside from these, many SCA patients suffer also from cognitive impairments and diverse mental issues, including apathy, depression and anxiety. Although often overlooked, growing evidence suggests their profound contribution to the reduced life quality and poor health outcomes. However, their nature remains largely unclear. This thesis aims to address this by studying the mouse models of hereditary ataxia. To achieve this, psychiatric-relevant behavioural abnormalities and their underlying neuropathology were studied in mice with cerebellar-specific degeneration (lurcher) and the knock-in mouse model of spinocerebellar ataxia 1 (SCA1 mice). The methodology included complex behavioural testing, histological techniques, biochemical analyses, immunofluorescence imaging, and measuring the mitochondrial oxidative metabolism. Obtained data were then analysed using modern statistical approaches accompanied by computer-intensive methods such as bootstrapping simulations. Experiments confirmed cognitive deficits in both lurcher and SCA1 mice. Interestingly, behavioural characterization identified numerous psychiatric-relevant behavioural...

host :: přihlásit Digitální repozitář
		Hledej		Nový záznam		Nápověda		O repozitáři