Název: Toxic responses in human lung epithelial cells (BEAS-2B) exposed to particulate matter exhaust emissions from gasoline and biogasoline
Autoři: Závodná, Táňa ; Líbalová, Helena ; Vrbová, Kristýna ; Sikorová, Jitka ; Vojtíšek-Lom, M. ; Beránek, V. ; Pechout, M. ; Kléma, J. ; Cigánek, M. ; Machala, M. ; Neča, J. ; Rössner ml., Pavel ; Topinka, Jan
Typ dokumentu: Příspěvky z konference
Konference/Akce: International Conference NANOCON 2020 /12./, Brno (CZ), 20201021
Rok: 2021
Jazyk: eng
Abstrakt: Motor vehicle emissions substantially contribute to air pollution worldwide and cause serious health problems. While the deleterious effects of diesel exhaust particulate matter (PM) have been widely studied, much less attention is paid to toxicity of PM emitted by gasoline engines although they also produce considerable amount of PM. The primary objective of this research was to assess toxic potencies of exhaust PM released by conventional gasoline engine fueled with neat gasoline (EU) or gasoline-ethanol blend (15% ethanol, v/v, E15). Despite a similar particle mass (mu g PM/kg fuel) produced by both fuels, PM emitted by E15 contained higher amount of harmful polycyclic aromatic hydrocarbons (PAH) as suggested by chemical analysis. To examine the toxicity of organic PM constituents, human lung BEAS-2B cells were exposed for 4h and 24h to a subtoxic dose of E0 and E15 PM organic extracts. We used genome scale transcriptomic analysis to characterize the toxic response and to identify modulated biological process and pathways. Whereas 4h exposure to both PM extracts resulted in modulation of similar genes and pathways related to lipid and steroid metabolism, activation of PPAR alpha, oxidative stress and immune response, 24h exposure was more specific for each extract, although both induced expression of PAH-metabolic enzymes, modulated metabolism of lipids or activated PPAR alpha, E15 additionally deregulated variety of other pathways. Overall, the PM mass produced by both fuels was similar, however, higher PAH content in E15 PM organic extract may have contributed to more extensive toxic response particularly after 24h exposure in BEAS-2B cells.
Klíčová slova: biofuels; gasoline; gene expression profiling; particulate matter emissions; toxicity
Číslo projektu: LM2015073 (CEP), GJ18-06548Y (CEP)
Poskytovatel projektu: GA MŠk, GA ČR
Zdrojový dokument: NANOCON 2020. 12th International Conference on Nanomaterials - Research & Application. Conference proceedings, ISBN 978-80-87294-98-7, ISSN 2694-930X
Poznámka: Související webová stránka: https://www.confer.cz/nanocon/2020/3763-toxic-responses-in-human-lung-epithelial-cells-beas-2b-exposed-to-particulate-matter-exhaust-emissions-from-gasoline-and-biogasoline

Instituce: Ústav experimentální medicíny AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: https://hdl.handle.net/11104/0333329

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-508688


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Věda a výzkum > AV ČR > Ústav experimentální medicíny
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