Original title: Variabilita v produkci extracelulárních fosfatáz dominantních druhů fytoplanktonu šumavských jezer
Translated title: Variability in extracellular phosphatase activity of dominant phytoplankton species in the Bohemian Forest lakes
Authors: Novotná, Jana ; Nedbalová, Linda (advisor) ; Rulík, Martin (referee)
Document type: Master’s theses
Year: 2008
Language: cze
Abstract: Phosphorus (P) is considered as the most frequently limiting factor for phytoplankton growth in freshwater environments. One of the ways, how to solve the lack of P in algae and cyanobacteria, is the production of extracellular phosphatases. Extracellular phosphatase activity (PA) of phytoplankton at the single cell level was investigated in three acidified mountain lakes - Čertovo, Prášilské and Plešné in the Bohemian Forest (Czech Republic) from May to September 2007. PA of phytoplankton was detected directly at the site of enzyme activity using the fluorescence labelled enzyme activity technique (FLEA) and epifluorescence microscopy. The FLEA technique is based on colourless substrate ELF phosphate (ELFP) that is converted by enzymatic hydrolysis to the insoluble fluorescent product ELF alcohol (ELFA), which marks the site of PA. This thesis is divided into two parts. The first part (in Czech) is aimed at a general comparison of PA of phytoplankton in the three Bohemian Forest lakes. PA was characteristic for dinoflagellates Gymnodinium uberrimum, Gymnodinium sp. and Peridinium umbonatum that were ELFA labelled in all lakes almost during the whole season. Other species (e.g. Carteria sp., Chlorogonium fusiforme or Synura sp.) were active either in only one of lakes or in some samples. PA was...

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/6766

Permalink: http://www.nusl.cz/ntk/nusl-499766


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses