Sojové isoflavonoidy, genistein a daidzein: Jejich biologické efekty na buněčné linie rakoviny prsu

Hůrková, Kristýna

- 1 - ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kristýna Hůrková Supervisors: Profa . Dra . Natércia Teixeira, Profa . Dra . Georgina Correia da Silva, Doc.Pharm.Dr. Tomáš Šimůnek Ph.D. Title of diploma thesis: Soya isoflavones, genistein and daidzein: Their biological effects on breast cancer cell lines Cancer is one of the leading causes of death worldwide. Epidemiological studies suggested that there is a low incidence of breast cancer in countries with high soya intake. Soya contains the isoflavones daidzein (D) and genistein (G), which are responsible for the protective properties, although their exact effects have not yet been clarified. In this study we evaluated and compared the biochemical and biological effects of a soya extract biotransformed by Aspergillus Awamori (SBE) and of the pure major isoflavones, G and D in placental microsomes and in an estrogen-dependent breast cancer cell line stably transfected with the aromatase gene, the MCF-7aro cell line. The results showed that D did not induce marked alterations in any of the parameters studied. In placental microsomes G was not a potent aromatase inhibitor, inducing only a moderate reduction in the aromatase activity. In MCF-7aro cells it was observed a significant...

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