Original title: An appropriate method for assessing hydrogel pore sizes by cryo-sem
Authors: Adámková, Kateřina ; Trudicová, M. ; Hrubanová, Kamila ; Sedláček, P. ; Krzyžánek, Vladislav
Document type: Papers
Conference/Event: Anniversary International Conference on Nanomaterials - Research and Application (NANOCON) /10./, Brno (CZ), 20181017
Year: 2019
Language: eng
Abstract: The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. \nReasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations, moreover, it showed more detailed and realistic structure.
Keywords: agarose; cryo-SEM; freezing methods; hydrogelcryo-SEM; image analysis
Project no.: GA17-15451S (CEP), LO1212 (CEP), ED0017/01/01
Funding provider: GA ČR, GA MŠk, GA MŠk
Host item entry: 10th Anniversary International Conference on Nanomaterials - Research and Application (NANOCON 2018), ISBN 978-80-87294-89-5

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0309123

Permalink: http://www.nusl.cz/ntk/nusl-432885


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