Název: Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
Autoři: Sushytskyi, L. ; Lukáč, Pavol ; Bleha, R. ; Čopíková, J. ; Vannucci, Luca ; Sinica, A. ; Kaštánek, P.
Typ dokumentu: Příspěvky z konference
Konference/Akce: The 15th International Conference on Polysaccharides-Glycoscience., Praha (CZ), 20191113
Rok: 2019
Jazyk: eng
Abstrakt: Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW (>1000 kDa and 98,3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column, and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnans, the latter one as branched xylogalactofuranan of unusual structure. CPS1 shown properties of unstable polimer: according to GPC analysis and by using light scattering and RI detectors it was found, that some fraction of polymers (260-800 kDa) have self-assembling properties in aqueous solution, and MW of this fraction decreased during some time while polimer with initially bigger MW (>1000 kDa) became bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98,3 kDa. Cell toxicity of the products were tested in vitro on cell cultures for different concentrations
Klíčová slova: cell cultures; Chlorella vulgaris G11; chromatography; Mutant alga; polymer
Číslo projektu: FV10155
Poskytovatel projektu: GA MPO
Zdrojový dokument: Proceedings of the 15th International Conference on Polysaccharides-Glycoscience, ISBN 978-80-88307-04-4

Instituce: Mikrobiologický ústav AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0312961

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-432307


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Mikrobiologický ústav
Konferenční materiály > Příspěvky z konference