Original title: Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Authors: Hlaváček, Antonín ; Křivánková, Jana ; Foret, František
Document type: Papers
Conference/Event: NANOCON 2019. International Conference on Nanomaterials - Research & Application /11./, Brno (CZ), 20191016
Year: 2020
Language: eng
Abstract: Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
Keywords: hyperspectral imaging; laser scanner; nanoparticle; photon-upconversion
Project no.: GJ18-03367Y (CEP)
Funding provider: GA ČR
Host item entry: NANOCON Conference Proceedings - International Conference on Nanomaterials, ISBN 978-808729495-6, ISSN 2694-930X
Note: Související webová stránka: https://www.confer.cz/nanocon/2019

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available in the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0311152

Permalink: http://www.nusl.cz/ntk/nusl-432156


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers