Original title: In vitro Incubation of the Labial Gland and Fat Body of the Bumblebee Males Bombus terrestris with [1,2-14C]acetate and Analysis of the Metabolites
Authors: Žáček, Petr ; Tykva, Richard ; Kindl, Jiří ; Valterová, Irena
Document type: Papers
Conference/Event: Proceedings of the International Students Conference "Modern Analytical Chemistry" /6./, Prague (CZ), 2010-09-23 / 2010-09-24
Year: 2010
Language: eng
Abstract: Labial glands and fat bodies of the bumblebee males species Bombus terrestris were incubated in the medium with sodium [1,2-14]acetate and [1,2-14]acetate addition. Metabolites of the accetate substrate were identified by means of localization of beta emission from compounds containing 14C in their structure on thin layer chromatography plates with non destructive position sensitive radio detector and comprehensive two dimensional gas chromatography (GCxGC-MS). It was found that radioactive [1,2-14]acetate incorporated into various kind of compounds such as terpenoids, fatty acids, hydrocarbons and triacylgylcerols.
Keywords: Bombus terrestris; comprehensive two dimensional gas chromatography; fat body; labial gland
Project no.: CEZ:AV0Z40550506 (CEP), GA203/09/1446 (CEP)
Funding provider: GA ČR
Host item entry: Proceedings of the 6th International Students Conference "Modern Analytical Chemistry", ISBN 978-80-7444-005-2

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0189471

Permalink: http://www.nusl.cz/ntk/nusl-41815


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
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