Original title: Vývoj a validace metody pro stanovení Cyklosporínu A v biorelevantních mediích
Translated title: Development and validation of method for determination of of Cyclosporine in biorelevant mediums
Authors: Tichoň, Matej ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Document type: Master’s theses
Year: 2020
Language: slo
Abstract: [eng] [cze]
Charles university Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Matej Tichoň Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of method for determination of cyclosporine A in biorelevant mediums For the determination of cyclosporine A in biorelevant mediums, a new analytical method was developed by using of ultra-high performance liquid chromatography. The analysis was performed on a Kinetex™ 1.7 µm, C18 column, 50 x 2.1 mm (Phenomenex, USA). The mobile phase consisted of ultrapure water acidified with phosphoric acid to pH = 2.40 and mixture of methanol : acetonitrile (90 : 10) in a total ratio of 30 : 70 aqueous to organic phases. The analysis was carried out at 60 řC for 5 minutes with a constant flow rate of 0.5 ml/min. Gradient elution was used in the analysis, starting at 70 % organic phase at 0 minutes and increasing to 100 % at 5 minutes. Cyclosporine A was detected at 210 nm. The retention time of the substance was 3.08 min. Having the optimized conditions in hands, the method was fully validated. Keywords: cyclosporine A, UHPLC, biorelevant mediums Univerzita Karlova Farmaceutická fakulta v Hradci Králové Katedra analytickej chémie Kandidát: Matej Tichoň Školiteľ: doc. PharmDr. Ludmila Matysová, Ph.D. Názov diplomovej práce: Vývoj a validácia metódy pre stanovenie cyklosporínu A v biorelevantných médiách Pre stanovenie cyklosporínu A v biorelevantných médiách bola vytvorená nová analytická metóda za použitia ultra-vysokoúčinnej kvapalinovej chromatografie. Analýza bola uskutočnená na kolóne KinetexTM 1,7 µm C18, 50 x 2,1 mm (Phenomenex, USA). Mobilná fáza bola tvorená ultračistou vodou okyslenou kyselinou fosforečnou na pH = 2,40 a zmesou metanol : acetonitril (90 : 10) v celkovom pomere vodnej a organickej fázy 30 : 70. Analýza prebiehala 5 minút pri teplote 60 řC s konštantným prietokom 0,5 ml/min. Pri analýze bola použitá gradientová elúcia, ktorá v čase 0 minút začínala koncentráciou 70 % organickej fázy a stúpala do času 5 minút na koncentráciu 100 %. Cyklosporín A bol detegovaný pri 210 nm. Retenčný čas látky bol 3,08 min. Po nájdení optimálnych podmienok bola metóda plne validovaná. Kľúčové slová: cyklosporín A, UHPLC, biorelevantné média

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/117408

Permalink: http://www.nusl.cz/ntk/nusl-411642


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses