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Original title:
SIZE AS AN IMPORTANT FACTOR IN NANO-TiO2 TOXICITY IN MACROPHAGE-LIKE CELLS
Authors: Líbalová, Helena ; Sikorová, Jitka ; Brzicová, Táňa ; Milcová, Alena ; Vrbová, Kristýna ; Pikal, P. ; Topinka, Jan ; Rössner ml., Pavel
Document type: Papers
Conference/Event: International Conference on Nanomaterials - Research and Application (NANOCON) /9./, Brno (CZ), 20171018
Year: 2018
Language: eng
Abstract: A set of NPs consists of 5 variants of anatase and 5 variants of rutile nanoparticles differing in their diameter (from 3 to 165 nm). TiO2 samples were characterized in the powder form and dispersed in water and cell culture media. Three cytotoxicity assays were used: MTS, WST-1, and LDH. For all nanomaterials, three independent repetitions were carried out. \n\nOverall, cytotoxicity of all NPs was low even at the highest concentration of 256 mu g/ml. The viability of cells did not decrease below 60% for WST-1 and MTS assays and 80% for the LDH assay. Besides concentration, crystalline size was identified as the most important cytotoxic factor. Clear nonlinear relationship between crystalline size and cytotoxicity was detected, higher toxicity induced NPs within the size range 20-60 nm. Increased cytotoxicity in given diameter size range would give an answer to inconsistent findings at size and cytotoxicity relationship.
Keywords: cytotoxicity; macrophages; nanoparticles; TiO2
Project no.: LO1508 (CEP), GBP503/12/G147 (CEP), LM2015073 (CEP)
Funding provider: GA MŠk, GA ČR, GA MŠk
Host item entry: Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application, ISBN 9788087294819
Institution: Institute of Experimental Medicine AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0302799
Permalink: http://www.nusl.cz/ntk/nusl-408932
The record appears in these collections:
Research > Institutes ASCR > Institute of Experimental Medicine
Conference materials > Papers
Authors: Líbalová, Helena ; Sikorová, Jitka ; Brzicová, Táňa ; Milcová, Alena ; Vrbová, Kristýna ; Pikal, P. ; Topinka, Jan ; Rössner ml., Pavel
Document type: Papers
Conference/Event: International Conference on Nanomaterials - Research and Application (NANOCON) /9./, Brno (CZ), 20171018
Year: 2018
Language: eng
Abstract: A set of NPs consists of 5 variants of anatase and 5 variants of rutile nanoparticles differing in their diameter (from 3 to 165 nm). TiO2 samples were characterized in the powder form and dispersed in water and cell culture media. Three cytotoxicity assays were used: MTS, WST-1, and LDH. For all nanomaterials, three independent repetitions were carried out. \n\nOverall, cytotoxicity of all NPs was low even at the highest concentration of 256 mu g/ml. The viability of cells did not decrease below 60% for WST-1 and MTS assays and 80% for the LDH assay. Besides concentration, crystalline size was identified as the most important cytotoxic factor. Clear nonlinear relationship between crystalline size and cytotoxicity was detected, higher toxicity induced NPs within the size range 20-60 nm. Increased cytotoxicity in given diameter size range would give an answer to inconsistent findings at size and cytotoxicity relationship.
Keywords: cytotoxicity; macrophages; nanoparticles; TiO2
Project no.: LO1508 (CEP), GBP503/12/G147 (CEP), LM2015073 (CEP)
Funding provider: GA MŠk, GA ČR, GA MŠk
Host item entry: Nanocon 2017 : conference proceedings : 9th International Conference on Nanomaterials - Research & Application, ISBN 9788087294819
Institution: Institute of Experimental Medicine AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0302799
Permalink: http://www.nusl.cz/ntk/nusl-408932
The record appears in these collections:
Research > Institutes ASCR > Institute of Experimental Medicine
Conference materials > Papers
Record created 2020-01-13, last modified 2022-09-29