Original title:
Analysis of Denatured PCR Products Modified with 7-deazapurine Bases at Hanging Mercury Drop Electrode
Authors:
Dudová, Zdenka ; Špaček, Jan ; Havran, Luděk ; Pivoňková, Hana ; Fojta, Miroslav Document type: Papers Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year:
2016
Language:
eng Abstract:
7-deazapunines are synthetic analogues of natural purine nucleobases which can pair with pyrimidines, retaining pairing complementarity of their parent purines. There is replaced N7 atom by CH group in 7-deazapurines so that DNA modified with them don't participate in Hoogsteen basepairing and don't form alternative structures. Here is a study of 7-deazapurines incorpotated into DNA measured at HMDE by CV and ACV. While 7-deazaadenine was reduced at the HMDE, giving rise to a similar irreversible cathodic peak as the natural adenine, 7-deazaguanine didn't yield any peak analogous to the peak G due to Guanine, in Agreement with a loss of corresponding redox in 7-deazaguanine.
Keywords:
dna; hmde; pcr; Voltammetry Project no.: GBP206/12/G151 (CEP) Funding provider: GA ČR Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5
Institution: Institute of Biophysics AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0297874