Název:
A Microfluidic devide for monitoring cell migration
Autoři:
Ledvina, Vojtěch ; Klepárník, Karel ; Legartová, Soňa ; Bártová, Eva Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Rok:
2018
Jazyk:
eng
Abstrakt: A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
Klíčová slova:
cell migration; microfluidic device; Premo FUCCI Cell Cycle Sensor Číslo projektu: GA17-01995S (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1